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A rapid and sensitive assay for histone acetyl-transferase activity

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dc.creatorAit-Si-Ali, S.spa
dc.creatorRamírez Clavijo, Sandra Rocíospa
dc.creatorRobin, P.spa
dc.creatorTrouche, D.spa
dc.creatorHarel-Bellan, A.spa
dc.date.accessioned2020-08-19T14:43:49Z
dc.date.available2020-08-19T14:43:49Z
dc.date.created1998-08-01spa
dc.description.abstractHistone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1093/nar/26.16.3869
dc.identifier.issnISSN: 0305-1048
dc.identifier.issnEISSN: 1362-4962
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27775
dc.language.isoengspa
dc.publisherOxford University Pressspa
dc.relation.citationEndPage3870
dc.relation.citationIssueNo. 16
dc.relation.citationStartPage3869
dc.relation.citationTitleNucleic Acids Research
dc.relation.citationVolumeVol. 26
dc.relation.ispartofNucleic Acids Research, ISSN: 0305-1048;EISSN: 1362-4962, Vol.26, No.16 (1 August 1998); pp. 3869–3870spa
dc.relation.urihttps://watermark.silverchair.com/26-16-3869.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAq4wggKqBgkqhkiG9w0BBwagggKbMIIClwIBADCCApAGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMimF92GpOeCO7A1OkAgEQgIICYQZsEVMIAXB0Y-56NtOexbqJAJnxIKDkCVqfk-UM_axWffOKl75mA-Gw4FUb5hb6cgkVhBukhhhsQzBo3Pn5BOh8fca5jDWhQTWsncSqhLAYgyfaq9wbAk4hSJvwkUvlktYq7SDYMUhIa8GoEdp1Zl0NI_cxoi5TszeAfORhH4scta8CZcoBRh076KeslNVAs6tt6u9DoWSnkoO_RvUwmOzK_JPDtWhwQT2VsE1n-jsqjty7K3bnxoJIysFJBM78ul3blTh14k8oE_gMYoOJNCVnZDpnYuq1mlNKk8XcBvPluCmPADuymVCtxSoIOudSw_OM0Rhg2MsxDDoTxG-JYtc94AFfIp8qnNQE7Dib4O5Ho-YBRkNTroKRVxgLD2e97Qg2s05f4-o3xmuV1DkZfW6tnjKCX5zFcj2UerStnFETDF8NS4vaIoKruNBQc7aOdIl7CtS4OooA6tEbVsM8ZYgLbL8d935RtdiljSoMqT4wH_pcF3-rrHf8RPUxc6jbilhLVMuzzxwqwdcm8uO4XmgNhq4X5U4m8RuaF93K5Wfm8kpRW7z5j8oVh6R0kG8cAytMkuNNcjZSGjtRcETKceY47GVvGv1G8vHWLU32KM7FomndOUeBCg0OUkpsEa0ZKsmsTxzW9ae_rsioNwHIGpavOyYrYuZB-WCt3M59roKYFK_Or1O8S2u7ZzXYqemcamWubnsUvSyg3Ks8by2pE1DP9Zy7Ul8WwvFeB-a34jK8U2QxDUzGROipyS8tWCRrAds6NrwJMnZlesPPIiMDcruZKZiDT98j626g1efKKTVKzgspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceNucleic Acids Researchspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordHATsspa
dc.subject.keywordEnzymesspa
dc.subject.keywordPeptidespa
dc.titleA rapid and sensitive assay for histone acetyl-transferase activityspa
dc.title.TranslatedTitleUn ensayo rápido y sensible para la actividad de la histona acetiltransferasaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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