Ítem
Solo Metadatos

Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis

Mostrar el registro sencillo de la publicación
dc.creatorChapeton-Montes, Jspa
dc.creatorPlaza, D.Fspa
dc.creatorBarrero, C.Aspa
dc.creatorPatarroyo, Manuel A.
dc.date.accessioned2020-08-19T14:40:20Z
dc.date.available2020-08-19T14:40:20Z
dc.date.created2008-01-01spa
dc.description.abstractCurrent methods for evaluating mycobacterial invasion of target cells pose technical difficulties including a long turn-around time. Thus, new methodologies must be developed that allow rapid and reliable monitoring of host cell invasion. Here, the invasion of A549 cell line by SYBR safe-labeled Mycobacterium tuberculosis (M. tuberculosis) H37Rv was assessed by flow cytometry and was expressed as the percentage of cells infected by M. tuberculosis. Over a third of A549 cells were invaded by M. tuberculosis, two hours after infection. The specificity of the invasion was confirmed in assays using red blood cells as target cells and Escherichia coli as the non-invasive bacterial control. These findings show that invasion of pulmonary epithelial cells by M. tuberculosis can rapidly and quantitatively be assessed with a great sensitivity by flow cytometric detection of SYBR safe-labeled M. tuberculosis.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.2741/2709
dc.identifier.issnISSN: 1093-9946
dc.identifier.issnEISSN: 1093-4715
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26834
dc.language.isoengspa
dc.publisherEuropa PMCspa
dc.relation.citationEndPage656
dc.relation.citationIssueNo. 3
dc.relation.citationStartPage650
dc.relation.citationTitleFrontiers in Bioscience, Frontiers in Bioscience - Landmark
dc.relation.ispartofFrontiers in Bioscience, Frontiers in Bioscience - Landmark, ISSN: 1093-9946; EISSN: 1093-4715, No.3 (2008); pp. 650-656spa
dc.relation.urihttps://europepmc.org/article/med/17981577spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceFrontiers in Bioscience, Frontiers in Bioscience - Landmarkspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordMycobacterial invasionspa
dc.subject.keywordHost cellspa
dc.subject.keywordMycobacterium tuberculosisspa
dc.titleQuantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosisspa
dc.title.TranslatedTitleMonitorización cuantitativa por citometría de flujo de la invasión de células epiteliales por Mycobacterium tuberculosisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Colecciones
Artículos