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Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection

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dc.creatorRoyle, J.spa
dc.creatorRamírez Santana, Heily Carolinaspa
dc.creatorAkpunarlieva, S.spa
dc.creatorDonald, C. L.spa
dc.creatorGestuveo, R. J.spa
dc.creatorAnaya, Juan-Manuelspa
dc.creatorMerits, A.spa
dc.creatorBurchmore, R.spa
dc.creatorKohl, A.spa
dc.creatorVarjak, M.spa
dc.date.accessioned2020-06-11T13:21:12Z
dc.date.available2020-06-11T13:21:12Z
dc.date.created2020spa
dc.description.abstractZika virus (ZIKV; Flaviviridae) is a mosquito-borne flavivirus shown to cause fetal abnormalities collectively known as congenital Zika syndrome and Guillain-Barré syndrome in recent outbreaks. Currently, there is no specific treatment or vaccine available, and more effort is needed to identify cellular factors in the viral life cycle. Here, we investigated interactors of ZIKV envelope (E) protein by combining protein pull-down with mass spectrometry. We found that E interacts with the endoplasmic reticulum (ER) resident chaperone, glucose regulated protein 78 (GRP78). Although other flaviviruses are known to co-opt ER resident proteins, including GRP78, to enhance viral infectivity, the role ER proteins play during the ZIKV life cycle is yet to be elucidated. We showed that GRP78 levels increased during ZIKV infection and localised to sites coincident with ZIKV E staining. Depletion of GRP78 using specific siRNAs significantly reduced reporter-virus luciferase readings, viral protein synthesis, and viral titres. Additionally, GRP78 depletion reduced the ability of ZIKV to disrupt host cell translation and altered the localisation of viral replication factories, though there was no effect on viral RNA synthesis. In summary, we showed GRP78 is a vital host-factor during ZIKV infection, which may be involved in the coordination of viral replication factories. © 2020 by the authors.eng
dc.format.mimetypeapplication/pdf
dc.identifier.issn19994915
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24770
dc.language.isoeng
dc.publisherMDPI AGspa
dc.relation.citationIssueNo. 5
dc.relation.citationTitleViruses
dc.relation.citationVolumeVol. 12
dc.relation.ispartofViruses, ISBN: 19994915, Vol.12, No.5 (2020); pp. -spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85084627200&doi=10.3390%2fv12050524&partnerID=40&md5=930e36e2d92a6252452b9173fec17154spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordGRP78spa
dc.subject.keywordProteomicsspa
dc.subject.keywordVirus-cell interactionsspa
dc.subject.keywordZika virusspa
dc.subject.keywordchaperonespa
dc.subject.keywordgallic acidspa
dc.subject.keywordglucosespa
dc.subject.keywordglucose regulated protein 78spa
dc.subject.keywordhonokiolspa
dc.subject.keywordluciferasespa
dc.subject.keywordmessenger RNAspa
dc.subject.keywordpotassium channelspa
dc.subject.keywordsmall interfering RNAspa
dc.subject.keywordtolonium chloridespa
dc.subject.keywordviral proteinspa
dc.subject.keywordvirus nucleoproteinspa
dc.subject.keywordantiviral activityspa
dc.subject.keywordArticlespa
dc.subject.keywordcell viabilityspa
dc.subject.keywordconfocal microscopyspa
dc.subject.keywordcontrolled studyspa
dc.subject.keywordgene expressionspa
dc.subject.keywordgene silencingspa
dc.subject.keywordgenetic transfectionspa
dc.subject.keywordhumanspa
dc.subject.keywordhuman cellspa
dc.subject.keywordimmunoblottingspa
dc.subject.keywordimmunofluorescence testspa
dc.subject.keywordimmunoprecipitationspa
dc.subject.keywordliquid chromatography-mass spectrometryspa
dc.subject.keywordmass spectrometryspa
dc.subject.keywordnonhumanspa
dc.subject.keywordprotein expressionspa
dc.subject.keywordprotein homeostasisspa
dc.subject.keywordprotein protein interactionspa
dc.subject.keywordprotein synthesisspa
dc.subject.keywordRNA synthesisspa
dc.subject.keywordupregulationspa
dc.subject.keywordvirus detectionspa
dc.subject.keywordvirus envelopespa
dc.subject.keywordvirus infectivityspa
dc.subject.keywordvirus loadspa
dc.subject.keywordvirus releasespa
dc.subject.keywordvirus replicationspa
dc.subject.keywordWestern blottingspa
dc.subject.keywordZika feverspa
dc.subject.keywordZika virusspa
dc.titleGlucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infectionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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