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The in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profile

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dc.creatorLópez, Carolinaspa
dc.creatorYepes-Pérez, Yoelisspa
dc.creatorDíaz Arévalo, Diana
dc.creatorPatarroyo, Manuel E.spa
dc.creatorPatarroyo, Manuel A.spa
dc.date.accessioned2020-05-26T00:08:21Z
dc.date.available2020-05-26T00:08:21Z
dc.date.created2018spa
dc.description.abstractMalaria caused by Plasmodium vivax is a neglected disease which is responsible for the highest morbidity in both Americas and Asia. Despite continuous public health efforts to prevent malarial infection, an effective antimalarial vaccine is still urgently needed. P. vivax vaccine development involves analyzing naturally-infected patients' immune response to the specific proteins involved in red blood cell invasion. The P. vivax rhoptry neck protein 2 (PvRON2) is a highly conserved protein which is expressed in late schizont rhoptries; it interacts directly with AMA-1 and might be involved in moving-junction formation. Bioinformatics approaches were used here to select B- and T-cell epitopes. Eleven high-affinity binding peptides were selected using the NetMHCIIpan-3.0 in silico prediction tool; their in vitro binding to HLA-DRB1*0401, HLA-DRB1*0701, HLA-DRB1*1101 or HLA-DRB1*1302 was experimentally assessed. Four peptides (39152 (HLA-DRB1*04 and 11), 39047 (HLA-DRB1*07), 39154 (HLADRB1*13) and universal peptide 39153) evoked a naturally-acquired T-cell immune response in P. vivax-exposed individuals from two endemic areas in Colombia. All four peptides had an SI greater than 2 in proliferation assays; however, only peptides 39154 and 39153 had significant differences compared to the control group. Peptide 39047 was able to significantly stimulate TNF and IL-10 production while 39154 stimulated TNF production. Allele-specific peptides (but not the universal one) were able to stimulate IL-6 production; however, none induced IFN-? production. The Bepipred 1.0 tool was used for selecting four B-cell epitopes in silico regarding humoral response. Peptide 39041 was the only one recognized by P. vivax-exposed individuals' sera and had significant differences concerning IgG subclasses; an IgG2 > IgG4 profile was observed for this peptide, agreeing with a protection-inducing role against P. falciparum and P. vivax as previously described for antigens such as RESA and MSP2. The bioinformatics results and in vitro evaluation reported here highlighted two T-cell epitopes (39047 and 39154) being recognized by memory cells and a B-cell epitope (39041) identified by P. vivax-exposed individuals' sera which could be used as potential candidates when designing a subunit-based vaccine. © 2018 López, Yepes-Pérez, Díaz-Arévalo, Patarroyo and Patarroyo.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3389/fcimb.2018.00156
dc.identifier.issn22352988
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24079
dc.language.isoengspa
dc.publisherFrontiers Media S.A.spa
dc.relation.citationIssueNo. MAY
dc.relation.citationTitleFrontiers in Cellular and Infection Microbiology
dc.relation.citationVolumeVol. 8
dc.relation.ispartofFrontiers in Cellular and Infection Microbiology, ISSN:22352988, Vol.8, No.MAY (2018)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85047117634&doi=10.3389%2ffcimb.2018.00156&partnerID=40&md5=3c2d0b3ab958e4fa878817f10b199d44spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAlkaline phosphatasespa
dc.subject.keywordhumoraleng
dc.subject.keywordGamma interferonspa
dc.subject.keywordHla drb1 antigenspa
dc.subject.keywordInterleukin 10spa
dc.subject.keywordInterleukin 6spa
dc.subject.keywordPlasmodium vivax rhoptry neck protein 2spa
dc.subject.keywordProteinspa
dc.subject.keywordStreptavidinspa
dc.subject.keywordTumor necrosis factorspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordCytokinespa
dc.subject.keywordEpitopespa
dc.subject.keywordHla drb1 antigenspa
dc.subject.keywordImmunoglobulin gspa
dc.subject.keywordParasite antigenspa
dc.subject.keywordPeptidespa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordAntigenicityspa
dc.subject.keywordArticlespa
dc.subject.keywordBioinformaticsspa
dc.subject.keywordCell isolationspa
dc.subject.keywordCell proliferationspa
dc.subject.keywordCytokine releasespa
dc.subject.keywordEnzyme linked immunospot assayspa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordHumoral immunityspa
dc.subject.keywordIc50spa
dc.subject.keywordImmune responsespa
dc.subject.keywordImmunofluorescencespa
dc.subject.keywordPeripheral blood mononuclear cellspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordWestern blottingspa
dc.subject.keywordBiologyspa
dc.subject.keywordBloodspa
dc.subject.keywordColombiaspa
dc.subject.keywordImmunologyspa
dc.subject.keywordMetabolismspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordPlasmodium vivax malariaspa
dc.subject.keywordAntigenseng
dc.subject.keywordCell proliferationspa
dc.subject.keywordColombiaspa
dc.subject.keywordComputational biologyspa
dc.subject.keywordCytokinesspa
dc.subject.keywordEpitopeseng
dc.subject.keywordEpitopeseng
dc.subject.keywordHla-drb1 chainsspa
dc.subject.keywordHumansspa
dc.subject.keywordImmunityeng
dc.subject.keywordImmunoglobulin gspa
dc.subject.keywordInhibitory concentration 50spa
dc.subject.keywordInterferon-gammaspa
dc.subject.keywordInterleukin-10spa
dc.subject.keywordInterleukin-6spa
dc.subject.keywordMalariaeng
dc.subject.keywordPeptidesspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordAntigenicityspa
dc.subject.keywordCellular and humoral responsespa
dc.subject.keywordEpitopespa
dc.subject.keywordHla-drb1 typingspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordPvron2spa
dc.subject.keywordSynthetic peptidespa
dc.titleThe in vitro antigenicity of Plasmodium vivax rhoptry neck protein 2 (PvRON2) B- and T-epitopes selected by HLA-DRB1 binding profilespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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