Ítem
Acceso Abierto

Evaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell lines

Mostrar el registro sencillo de la publicación
dc.creatorRangel N.spa
dc.creatorRondón M.spa
dc.creatorRamírez Clavijo, Sandra Rocíospa
dc.date.accessioned2020-05-26T00:04:52Z
dc.date.available2020-05-26T00:04:52Z
dc.date.created2009spa
dc.description.abstractBackground: The incidence of malign melanoma tumours has increased more rapidly than any other type of cancer; this has intensified the searching for tools that facilitate early detection of melanoma. Microphthalmia associated transcription factor (MITF) is currently known as being a master melanocyte regulator. The article analyses MITF gene expression in peripheral blood of individuals suffering from melanoma, compared to people without any type of cancer and some cell lines. Materials and methods: Thirty one samples of peripheral blood were used: 19 from patients having melanoma and 12 from healthy people. Then RNA was extracted from these samples. MITF and housekeeping genes (?2M and GAPDH) expression levels were then quantified by real-time PCR. Five cell lines were also used to determine the MITF expression. Results: MITF gene expression could be observed in all individuals, though no statistical significant differences were found among expression levels in the groups studied (p=0.09). Even so, MITF expression in the group of patients suffering from melanoma was much more variable than that observed in the group of cancer-free people. Expression was detected in the cell line AGS (gastric adenocarcinoma), not yet described. Conclusions: MITF gene expression levels were detected in the peripheral blood from both people suffering from melanoma and people without any type of cancer. However, variability in the number of molecules in MITF gene expression was observed in people with melanoma, this suggests the presence of tumour cells in circulation. © 2009 Universidad del Valle, Facultad de Salud.eng
dc.format.mimetypeapplication/pdf
dc.identifier.issn16579534
dc.identifier.issn01208322
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23728
dc.language.isoengspa
dc.relation.citationEndPage33
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage16
dc.relation.citationTitleColombia Medica
dc.relation.citationVolumeVol. 40
dc.relation.ispartofColombia Medica, ISSN:16579534, 01208322, Vol.40, No.1 (2009); pp. 16-33spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-64549093414&partnerID=40&md5=3a75af35d9c919580d61ed839cbae02fspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordMicrophthalmia associated transcription factorspa
dc.subject.keywordRnaspa
dc.subject.keywordArticlespa
dc.subject.keywordBlood samplingspa
dc.subject.keywordCancer cell culturespa
dc.subject.keywordCirculationspa
dc.subject.keywordClinical articlespa
dc.subject.keywordControlled studyspa
dc.subject.keywordGene expressionspa
dc.subject.keywordHousekeeping genespa
dc.subject.keywordHumanspa
dc.subject.keywordMelanomaspa
dc.subject.keywordProtein blood levelspa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordRna extractionspa
dc.subject.keywordStomach adenocarcinomaspa
dc.subject.keywordTumor cellspa
dc.subject.keywordCancerspa
dc.subject.keywordCirculation tumor cellsspa
dc.subject.keywordMelanomaspa
dc.subject.keywordMitfspa
dc.subject.keywordQpcrspa
dc.titleEvaluation of microphthalmia associated transcription factor (MITF) expression in peripheral blood of a population with malign melanoma and control population and cell linesspa
dc.title.TranslatedTitleEvaluacuón de la expresión del factor de transcripción asociado con microftalmia (MITF) en sangre periférica de individuos con y sin melanoma maligno y en líneas celularesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
626-Manuscript-1902-1-10-20091202.pdf
Tamaño:
225.78 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos