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Caracterización de la microbiota intestinal de bovinos raza Holstein parasitados por Fasciola spp. en Cundinamarca y Boyacá, Colombia.

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dc.contributorGarcía, Diego
dc.contributorPulido, Martín
dc.contributorGiraldo, Julio Cesar
dc.contributorHerrera, Giovanny
dc.contributorMuñoz, Marina
dc.contributorCruz-Saavedra, Lissa
dc.contributor.advisorRamírez, Juan David
dc.creatorRamírez Hernández, Angie Lorena
dc.creator.degreeBiólogospa
dc.creator.degreetypeFull timespa
dc.date.accessioned2021-02-17T13:20:08Z
dc.date.available2021-02-17T13:20:08Z
dc.date.created2021-01-30
dc.descriptionLa identificación de alteraciones en la composición de la microbiota intestinal asociada a la presencia de parásitos ha sido un foco de atención de investigaciones por su importancia en procesos fisiológicos y de productividad animal. Dentro del campo de las relaciones hospedero-parásito, muchos estudios han sugerido que los helmintos pueden incidir sobre la composición microbiana debido a su efecto inmunomodulador. La fascioliasis bovina es una helmintiasis ampliamente estudiada en el campo de la inmunología, pero con poca información en el campo de la investigación de interacciones hospedero-helminto, por lo que se desconoce su impacto sobre las comunidades microbianas. Por esta razón, este estudio se dirige a describir la composición de la microbiota intestinal de bovinos Holstein parasitados por Fasciola provenientes del altiplano cundiboyacense colombiano, a través de un enfoque de secuenciación de nueva generación de amplicones de los genes ARNr-16S para procariotas y ARNr-18S para eucariotas. Para esto, se recolectaron 65 muestras fecales de bovinos Holstein provenientes de plantas de beneficio animal. Encontramos reducción en la abundancia relativa de Bacteroidetes y Ascomycota en las muestras de bovinos infectadas con el parásito, así como disminución en abundancia relativa de taxones comensales asociados a procesos de fermentación y digestión. Adicionalmente, se encontraron correlaciones positivas entre microorganismos que presentaron reducciones en abundancias relativas en las muestras positivas para Fasciola, lo cual se asoció a pérdida de peso consistente con el fenotipo de un bovino infectado por este parásito. Por lo anterior, se concluyó que la infección por Fasciola disminuye la capacidad de digerir material vegetal como consecuencia de la reducción en la abundancia de taxones encargados de esta función. Estos hallazgos proveen una base para futuras investigaciones sobre el desarrollo de tratamientos dirigidos a la modulación de estas poblaciones microbianas implicadas en la infección.spa
dc.description.abstractAlterations in gut microbiota associated with the presence of parasites have been a focus of research attention due to its importance in physiological processes and animal productivity. Within the field of host-parasite relationships, many studies have suggested that helminths can influence microbial composition due to their immunomodulatory effect. Bovine fascioliasis is helminthiasis widely studied in the field of immunology, but with few information in the field of host-helminth interactions, so its impact on microbial communities is unknown. For this reason, this study aims to describe the composition of the gut microbiota of Holstein cattle parasitized by Fasciola in the Colombian Cundiboyacense highlands through a new generation sequencing approach of rRNA-16S gene amplicons for prokaryotes and rRNA. -18S for eukaryotes. Thus, 65 fecal samples of Holstein cattle from animal benefit plants were collected. We found a reduction in the relative abundance of Bacteroidetes and Ascomycota in the samples of bovines infected with the parasite, as well as a decrease in the relative abundance of commensal taxa associated with fermentation and digestion processes. Additionally, we found positive correlations between microorganisms that presented reductions in relative abundances in the samples positive for Fasciola, which was associated with weight loss consistent with the phenotype of a bovine infected by this parasite. Therefore, we concluded that Fasciola infection decreases the ability to digest plant material in bovines as a consequence of the reduction in the abundance of taxa responsible for this function. These findings provide a basis for future research on the development of treatments aimed at modulating these microbial populations involved in infection.spa
dc.description.sponsorshipBolsa de apoyo a estudiantes de pregrado de la Facultad de Ciencias Naturalesspa
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.48713/10336_30926
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/30926
dc.language.isospaspa
dc.publisherUniversidad del Rosariospa
dc.publisher.departmentFacultad de Ciencias Naturales y Matemáticasspa
dc.publisher.programBiologíaspa
dc.rightsAtribución-SinDerivadas 2.5 Colombiaspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.spa
dc.rights.urihttp://creativecommons.org/licenses/by-nd/2.5/co/
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dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectFasciolosis hepática en bovinosspa
dc.subjectCambios en la microbiota intestinal bobina por helmintiasisspa
dc.subjectCambios microbiológicos por enfermedades parasitariasspa
dc.subject.ddcMicrobiologíaspa
dc.subject.keywordHepatic fasciolosis in cattlespa
dc.subject.keywordChanges in the intestinal microbiota coil due to helminthiasisspa
dc.subject.keywordMicrobiological changes due to parasitic diseasesspa
dc.titleCaracterización de la microbiota intestinal de bovinos raza Holstein parasitados por Fasciola spp. en Cundinamarca y Boyacá, Colombia.spa
dc.title.TranslatedTitleCharacterization of intestinal microbiota of Holstein cattle parasitized and non-parasitized by Fasciola spp. in Cundinamarca and Boyacá, Colombiaeng
dc.typebachelorThesiseng
dc.type.documentArtículospa
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de gradospa
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