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Caracterización del transcriptoma de la araña polimórfica de color Gasteracantha cacriformis, con enfoque a los genes de pigmento

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dc.contributor.advisorPardo Díaz, Geimy Carolina
dc.creatorTorres Quintero, Paula Alexandra
dc.creator.degreeBiólogospa
dc.creator.degreetypeFull timespa
dc.date.accessioned2020-08-20T17:52:20Z
dc.date.available2020-08-20T17:52:20Z
dc.date.created2020-07-24
dc.descriptionEl polimorfismo de coloración es un sistema útil para estudiar procesos evolutivos y de desarrollo. No obstante, el estudio de este rasgo se ha sesgado a organismos modelo y la coloración en arácnidos no ha sido bien estudiada. La araña Gasteracantha cancriformis es una especie neotropical que presenta polimorfismo de coloración abdominal con al menos 16 morfos conocidos distribuidos desde el sur de Estados Unidos hasta el norte de Argentina. A la fecha, las bases genéticas que controlan dicho polimorfismo son desconocidas. En este estudio implementé RNAseq para ensamblar el transcriptoma de Gasteracantha, y evaluar expresión génica diferencial asociada a la coloración en hembras de color blanco, amarillo y naranja. A partir de las secuencias de los transcritos hice análisis nucleotídicos y estadísticos para establecer si existe una relación entre coloración y polimorfismos de ADN (SNPs) en el transcriptoma. En general, el perfil de expresión génica fue similar entre los fenotipos amarillo y naranja, quienes difirieron del fenotipo blanco. En los morfos amarillo y naranja dectecté mayor expresión de genes relacionados a astacina y vitelogenina, lo cual sugiere que estas coloraciones en G. cancriformis son producto de carotenoides. A nivel nucleotídico encontré que los transcritos diferencialmente expresados muestran señal asociada a fenotipo de color, pero esto no sucede a nivel de transcriptoma completo. Los datos obtenidos en este estudio proporcionan un recurso genético valioso para futuras investigaciones en arañas neotropicales y constituyen un paso fundamental hacia la identificación de las bases genéticas del polimorfismo de color en estos animales.spa
dc.description.abstractColor polymorphism in animals is a useful system to study evolutionary and developmental processes. However, the study of this trait is biased towards model organisms, while coloration in arachnids is less explored. Gasteracantha cancriformis is a neotropical spider that displays color polymorphism with at least 16 known morphs distributed from the southern United States to northern Argentina but, to date, the genetic basis underlying such polymorphism is unknown. In this study, I used RNAseq to generate a transcriptome assembly for Gasteracantha and assess differential gene expression between three different color morphs: white, yellow and orange. I also explored whether single nucleotide polymorphism (SNPs) across the transcriptome display any association with coloration. Overall, the gene expression profile of the yellow and orange morphs was similar, and they both differed from the white morph. Consistently, at nucleotide level I found that SNPs in the differential expressed transcripts cluster orange and yellow morphs together while the white morph appears more differentiated. Also, I detected higher expression levels of astacin and vitellogenin genes in the yellow and orange morphs, suggesting that these colorations in G. cancriformis are due to carotenoids. The data obtained in this study provide a valuable genetic resource for future studies on neotropical spiders and constitute a step towards the identification of the genetic basis of color polymorphism in these animals.spa
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dc.identifier.doihttps://doi.org/10.48713/10336_28197
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/28197
dc.language.isospaspa
dc.publisherUniversidad del Rosariospa
dc.publisher.departmentFacultad de Ciencias Naturales y Matemáticasspa
dc.publisher.programBiologíaspa
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dc.rightsAtribución-NoComercial-SinDerivadas 2.5 Colombiaspa
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dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectGasteracantha cancriformisspa
dc.subjectTranscriptomaspa
dc.subjectExpresión diferencial de genesspa
dc.subjectPolimorfismo de colorspa
dc.subject.ddcInvertebradosspa
dc.subject.ddcEvolución & genéticaspa
dc.subject.keywordGasteracantha cancriformiseng
dc.subject.keywordGasteracantha cancriformisspa
dc.subject.keywordTranscriptomespa
dc.subject.keywordDifferential gene expressionspa
dc.subject.keywordColor polymorphismspa
dc.titleCaracterización del transcriptoma de la araña polimórfica de color Gasteracantha cacriformis, con enfoque a los genes de pigmentospa
dc.title.TranslatedTitleCharacterization of the transcriptome of color-polymorphic spider Gasteracantha cancriformis with special reference to pigment genes.eng
dc.typebachelorThesiseng
dc.type.documentMonografíaspa
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de gradospa
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