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Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets

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dc.creatorCamargo M.spa
dc.creatorSoto-De Leon S.spa
dc.creatorSanchez R.spa
dc.creatorMuñoz, Marinaspa
dc.creatorVega E.spa
dc.creatorBeltran M.spa
dc.creatorPerez-Prados A.spa
dc.creatorPatarroyo M.E.spa
dc.creatorPatarroyo M.A.spa
dc.date.accessioned2020-05-25T23:56:43Z
dc.date.available2020-05-25T23:56:43Z
dc.date.created2011spa
dc.description.abstractThe aims of this study were to determine the prevalence of HPV infection and evaluate the concordance and performance of two primer sets for detecting single and multiple viral infections. A total of 1810 Colombian women were enrolled in the study, and molecular, cytological and epidemiological analyses were performed. Both concordance and performance of two different PCR amplification primer sets (GP5+/6+ and MY09/11) were assessed. The results showed that 60.2% of females with positive HPV DNA were infected by more than one viral type. The OR for multiple infections was 18.2 when using the MY09/11 primer set and 6.52 with the GP5+/6+ primer set. The results also showed an association between GP5+/6+ positivity and the severity of the disease regarding the cytological findings. It was also found that using a single primer set led to underestimating the prevalence for HPV infection. The simultaneous use of these primer sets is an important tool for the detection of HPV DNA, being equally relevant for identifying multiple infections and low viral DNA copies. This study highlights the importance of suitable assessment of HPV epidemiological profiles; screening programs must also be strengthened to broaden the coverage of the most vulnerable populations. © 2011 Elsevier B.V.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jviromet.2011.08.014
dc.identifier.issn1660934
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22493
dc.language.isoengspa
dc.relation.citationEndPage74
dc.relation.citationIssueNo. 43862
dc.relation.citationStartPage68
dc.relation.citationTitleJournal of Virological Methods
dc.relation.citationVolumeVol. 178
dc.relation.ispartofJournal of Virological Methods, ISSN:1660934, Vol.178, No.43862 (2011); pp. 68-74spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-80955142754&doi=10.1016%2fj.jviromet.2011.08.014&partnerID=40&md5=4a23c7f46d9e50cdb06db00513674560spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordVirus DNAspa
dc.subject.keywordAdultspa
dc.subject.keywordAgedspa
dc.subject.keywordArticlespa
dc.subject.keywordBeta globin genespa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCytologyspa
dc.subject.keywordDisease severityspa
dc.subject.keywordFemalespa
dc.subject.keywordGenespa
dc.subject.keywordGene dosagespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman tissuespa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMixed infectionspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPapillomavirus infectionspa
dc.subject.keywordPerformance measurement systemspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPrevalencespa
dc.subject.keywordPriority journalspa
dc.subject.keywordScreening testspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordVirus detectionspa
dc.subject.keywordVirus loadspa
dc.subject.keywordWart virusspa
dc.subject.keywordAdolescentspa
dc.subject.keywordAdultspa
dc.subject.keywordAgedspa
dc.subject.keywordCoinfectionspa
dc.subject.keywordColombiaspa
dc.subject.keywordDNA Primersspa
dc.subject.keywordFemalespa
dc.subject.keywordHumansspa
dc.subject.keywordMiddle Agedspa
dc.subject.keywordMolecular Diagnostic Techniquesspa
dc.subject.keywordPapillomaviridaespa
dc.subject.keywordPapillomavirus Infectionsspa
dc.subject.keywordPolymerase Chain Reactionspa
dc.subject.keywordVirologyspa
dc.subject.keywordYoung Adultspa
dc.subject.keywordHuman papillomavirusspa
dc.subject.keywordColombiaspa
dc.subject.keywordGP5+/6+spa
dc.subject.keywordHuman papillomavirus (HPV)spa
dc.subject.keywordMultiple infectionsspa
dc.subject.keywordMY09/11spa
dc.titleDetection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer setsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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