Ítem
Acceso Abierto

Molecular epidemiology of human oral chagas disease outbreaks in Colombia

Mostrar el registro sencillo de la publicación
dc.creatorRamírez, Juan Davidspa
dc.creatorMontilla, Marlenyspa
dc.creatorCucunubá, Zulma M.spa
dc.creatorFloréz, Astrid Carolinaspa
dc.creatorZambrano, Pilarspa
dc.creatorGuhl, Felipespa
dc.date.accessioned2020-08-19T14:46:06Z
dc.date.available2020-08-19T14:46:06Z
dc.date.created2013-02-21spa
dc.description.abstractBackground Trypanosoma cruzi, the causative agent of Chagas disease, displays significant genetic variability revealed by six Discrete Typing Units (TcI-TcVI). In this pathology, oral transmission represents an emerging epidemiological scenario where different outbreaks associated to food/beverages consumption have been reported in Argentina, Bolivia, Brazil, Ecuador and Venezuela. In Colombia, six human oral outbreaks have been reported corroborating the importance of this transmission route. Molecular epidemiology of oral outbreaks is barely known observing the incrimination of TcI, TcII, TcIV and TcV genotypes. Methodology and Principal Findings High-throughput molecular characterization was conducted performing MLMT (Multilocus Microsatellite Typing) and mtMLST (mitochondrial Multilocus Sequence Typing) strategies on 50 clones from ten isolates. Results allowed observing the occurrence of TcI, TcIV and mixed infection of distinct TcI genotypes. Thus, a majority of specific mitochondrial haplotypes and allelic multilocus genotypes associated to the sylvatic cycle of transmission were detected in the dataset with the foreseen presence of mitochondrial haplotypes and allelic multilocus genotypes associated to the domestic cycle of transmission. Conclusions These findings suggest the incrimination of sylvatic genotypes in the oral outbreaks occurred in Colombia. We observed patterns of super-infection and/or co-infection with a tailored association with the severe forms of myocarditis in the acute phase of the disease. The transmission dynamics of this infection route based on molecular epidemiology evidence was unraveled and the clinical and biological implications are discussed.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0002041
dc.identifier.issnISSN: 1935-2727
dc.identifier.issnEISSN: 1935-2735
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/28149
dc.language.isoengspa
dc.publisherPLOS Public Library of Sciencespa
dc.relation.citationIssueNo. 2
dc.relation.citationStartPagee2041
dc.relation.citationTitlePLoS Neglected Tropical Diseases
dc.relation.citationVolumeVol. 7
dc.relation.ispartofPLoS Neglected Tropical Diseases, ISSN: 1935-2727;EISSN: 1935-2735, Vol.7, No.2 (February 2013); pp. e2041spa
dc.relation.urihttps://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0002041&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourcePLoS Neglected Tropical Diseasesspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordTrypanosoma cruzispa
dc.subject.keywordCloningspa
dc.subject.keywordChagas diseasespa
dc.subject.keywordEpidemiologyspa
dc.subject.keywordHaplotypesspa
dc.subject.keywordOral diseasesspa
dc.subject.keywordColombiaspa
dc.subject.keywordMitochondriaspa
dc.titleMolecular epidemiology of human oral chagas disease outbreaks in Colombiaspa
dc.title.TranslatedTitleEpidemiología molecular de los brotes de enfermedad de chagas oral en humanos en Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
journal-pntd-0002041.pdf
Tamaño:
336.11 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos