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Simultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximab

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dc.creatorHerrera, Daniel
dc.creatorRojas, Olga L.
dc.creatorDuarte-Rey, Carolina
dc.creatorMantilla, Rubén D.
dc.creatorÁngel, Juana
dc.creatorFranco, Manuel A.
dc.creator.googleHerrera, Danielspa
dc.creator.googleRojas, Olga L.spa
dc.creator.googleDuarte-Rey, Carolinaspa
dc.creator.googleMantilla, Rubén D.spa
dc.creator.googleÁngel, Juanaspa
dc.creator.googleFranco, Manuel A.spa
dc.date.accessioned2018-11-19T16:18:47Z
dc.date.available2018-11-19T16:18:47Z
dc.date.created2014
dc.date.issued2014
dc.description.abstractThe mechanisms that contribute to the maintenance of serological memory are still unclear. Rotavirus (RV) memory B cells (mBc) are enriched in IgM + and CD27- subpopulations, which are associated with autoimmune diseases pathogenesis. In patients with autoimmune diseases treated with Rituximab (RTX), some autoantibodies (auto-Abs) decrease after treatment, but other auto-Abs and pathogen-specific IgG Abs remain unchanged. Thus, maintenance of autoimmune and pathogenspecific serological memory may depend on the type of antigen and/or Ab isotype evaluated. Antigen-specific mBc and antigen-specific Abs of different isotypes have not been simultaneously assessed in patients after RTX treatment. To study the relationship between mBc subpopulations and serological memory we characterized total, RV- and tetanus toxoid (TT)-specific mBc by flow cytometry in patients with autoimmune diseases before and after treatment with RTX. We also measured total, RV- and TT-Abs, and some auto-Abs by kinetic nephelometry, ELISA, and EliA tests, respectively. Minor differences were observed between the relative frequencies of RV-mBc in healthy controls and patients with autoimmune disease. After RTX treatment, naïve Bc and total, RV- and TT-specific mBc [IgM+, switched (IgA+/IgG+), IgM+ only, IgD+ only, and CD27- (IgA+/IgG+/IgM+)] were significantly diminished. An important decrease in total plasma IgM and minor decreases in total IgG and IgA levels were also observed. IgM rheumatoid factor, IgG anti-CCP, and IgG anti-dsDNA were significantly diminished. In contrast, RV-IgA, RV-IgG and RV-IgG1, and TT-IgG titers remained stable. In conclusion, in patients with autoimmunity, serological memory against RV and TT seem to be maintained by long-lived plasma cells, unaffected by RTX, and an important proportion of total IgM and serological memory against some auto-antigens seem to be maintained by short-lived plasma cells, dependent on mBc precursors depleted by RTX. © 2014 Herrera et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0097087
dc.identifier.issn1932-6203
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18703
dc.language.isoengspa
dc.relation.citationIssueNo. 5
dc.relation.citationTitlePLoS ONE
dc.relation.citationVolumeVol. 9
dc.relation.ispartofPLoS ONE, ISSN: 1932-6203, Vol. 9/No. 5 (2014)spa
dc.relation.urihttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0097087&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/spa
dc.source.bibliographicCitationAmanna, I.J., Carlson, N.E., Slifka, M.K., Duration of humoral immunity to common viral and vaccine antigens (2007) New England Journal of Medicine, 357 (19), pp. 1903-1915. , http://content.nejm.org/cgi/reprint/357/19/1903.pdf, DOI 10.1056/NEJMoa066092spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordTetanus Toxoideng
dc.subject.keywordRheumatoid Factoreng
dc.subject.keywordMethylprednisoloneeng
dc.subject.keywordMiddle Agedeng
dc.subject.keywordMonoclonal Antibodyeng
dc.subject.keywordFemalespa
dc.subject.keywordHumansspa
dc.subject.keywordMiddle Agedspa
dc.subject.keywordSpecificityeng
dc.subject.keywordSpecieseng
dc.subject.keywordRotaviruseng
dc.subject.keywordLymphocyte Depletioneng
dc.subject.keywordImmunologic Memoryeng
dc.subject.keywordImmunoglobulin Meng
dc.subject.keywordB-Lymphocyteseng
dc.subject.keywordB-Lymphocyte Subsetseng
dc.subject.keywordAutoantigenseng
dc.subject.keywordAntibodieseng
dc.subject.keywordAgedeng
dc.subject.keywordSpecies Differenceeng
dc.subject.keywordRotaviruseng
dc.subject.keywordProcedureseng
dc.subject.keywordLymphocyte Depletioneng
dc.subject.keywordDrug Effectseng
dc.subject.keywordSystemic Lupus Erythematosuseng
dc.subject.keywordRheumatoid Arthritiseng
dc.subject.keywordNephelometryeng
dc.subject.keywordErythematosus Nephritiseng
dc.subject.keywordKinetic Nephelometryeng
dc.subject.keywordLupuseng
dc.subject.keywordCd27 Antigeneng
dc.subject.keywordDouble Stranded Dna Antibodyeng
dc.subject.keywordRituximabeng
dc.subject.keywordAntigen Specificityeng
dc.subject.keywordFlow Cytometryeng
dc.subject.keywordEnzyme Linked Immunosorbent Assayeng
dc.subject.keywordAutoimmune Thrombocytopeniaeng
dc.subject.keywordMonoclonaleng
dc.subject.keywordAntiphospholipid Syndromeeng
dc.subject.keywordImmunoglobulin Blood Leveleng
dc.subject.lembRotavirusspa
dc.subject.lembLinfocitos Bspa
dc.subject.lembRituximabspa
dc.titleSimultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximabspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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