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Genetic structure of Trypanosoma cruzi in Colombia revealed by a High-throughput Nuclear Multilocus Sequence Typing (nMLST) approach

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dc.creatorRamírez, Juan Davidspa
dc.creatorTapia-Calle, Gabrielaspa
dc.creatorGuhl, Felipespa
dc.date.accessioned2020-08-19T14:43:48Z
dc.date.available2020-08-19T14:43:48Z
dc.date.created2013-09-30spa
dc.description.abstractBackground Chagas disease is a systemic pathology caused by Trypanosoma cruzi. This parasite reveals remarkable genetic variability, evinced in six Discrete Typing Units (DTUs) named from T. cruzi I to T. cruzi VI (TcI to TcVI). Recently newly identified genotypes have emerged such as TcBat in Brazil, Colombia and Panama associated to anthropogenic bats. The genotype with the broadest geographical distribution is TcI, which has recently been associated to severe cardiomyopathies in Argentina and Colombia. Therefore, new studies unraveling the genetic structure and natural history of this DTU must be pursued. Results We conducted a spatial and temporal analysis on 50 biological clones of T. cruzi I (TcI) isolated from humans with different clinical phenotypes, triatomine bugs and mammal reservoirs across three endemic regions for Chagas disease in Colombia. These clones were submitted to a nuclear Multilocus Sequence Typing (nMLST) analysis in order to elucidate its genetic diversity and clustering. After analyzing 13 nuclear housekeeping genes and obtaining a 5821 bp length alignment, we detected two robust genotypes within TcI henceforth named TcIDOM (associated to human infections) and a second cluster associated to peridomestic and sylvatic populations. Additionaly, we detected putative events of recombination and an intriguing lack of linkage disequilibrium. Conclusions These findings reinforce the emergence of an enigmatic domestic T. cruzi genotype (TcIDOM), and demonstrates the high frequency of recombination at nuclear level across natural populations of T. cruzi. Therefore, the need to pursue studies focused on the diferential virulence profiles of TcI strains. The biological and epidemiological implications of these findings are herein discussed.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/1471-2156-14-96
dc.identifier.issnEISSN: 1471-2156
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27772
dc.language.isoengspa
dc.publisherBioMed Centralspa
dc.relation.citationIssueNo. 96
dc.relation.citationTitleBMC Genetics
dc.relation.citationVolumeVol. 14
dc.relation.ispartofBMC Genetics, EISSN: 1471-2156, Vol.14, No.96 (2013); 11 pp.spa
dc.relation.urihttps://bmcgenet.biomedcentral.com/track/pdf/10.1186/1471-2156-14-96spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceBMC Geneticsspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordChagas diseasespa
dc.subject.keywordClonalityspa
dc.subject.keywordSexualityspa
dc.subject.keywordDisease ecologyspa
dc.subject.keywordTransmission dynamicsspa
dc.subject.keywordGenotypesspa
dc.titleGenetic structure of Trypanosoma cruzi in Colombia revealed by a High-throughput Nuclear Multilocus Sequence Typing (nMLST) approachspa
dc.title.TranslatedTitleEstructura genética de Trypanosoma cruzi en Colombia revelada por un enfoque de tipificación de secuencia nuclear multilocus de alto rendimiento (nMLST)spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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