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Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels

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dc.creatorde-la-Torre, Alejandra
dc.creatorSauer, Arnaud
dc.creatorPfaff, Alexander W.
dc.creatorBourcier, Tristan
dc.creatorBrunet, Julie
dc.creatorSpeeg-Schatz, Claude
dc.creatorBallonzoli, Laurent
dc.creatorVillard, Odile
dc.creatorAjzenberg, Daniel
dc.creatorSundar, Natarajan
dc.creatorGrigg, Michael E.
dc.creatorGomez-Marin, Jorge E.
dc.creatorCandolfi, Ermanno
dc.creator.googlede-la-Torre, Alejandraspa
dc.creator.googleSauer, Arnaudspa
dc.creator.googlePfaff, Alexander W.spa
dc.creator.googleBourcier, Tristanspa
dc.creator.googleBrunet, Juliespa
dc.creator.googleBallonzoli, Laurentspa
dc.creator.googleVillard, Odilespa
dc.creator.googleAjzenberg, Danielspa
dc.creator.googleSundar, Natarajanspa
dc.creator.googleGrigg, Michael E.spa
dc.creator.googleGomez-Marin, Jorge E.spa
dc.creator.googleCandolfi, Ermannospa
dc.date.accessioned2018-11-22T19:33:16Z
dc.date.available2018-11-22T19:33:16Z
dc.date.created2013
dc.date.issued2013
dc.description.abstractIn a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/doi:10.1371/journal.pntd.0002541
dc.identifier.issn1935-2727
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18733
dc.language.isoengspa
dc.relation.citationIssueNo. 11
dc.relation.citationTitlePLoS Neglected Tropical Diseases
dc.relation.citationVolumeVol. 7
dc.relation.ispartofPLoS Neglected Tropical Diseases, ISSN: 1935-2727, Vol. 7/No. 11 (2013)spa
dc.relation.urihttps://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0002541&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/spa
dc.source.bibliographicCitationArevalo, J.F., Belfort Jr., R., Muccioli, C., Espinoza, J.V., Ocular toxoplasmosis in the developing world (2010) Int Ophthalmol Clin, 50, pp. 57-69spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectInterferón gammaspa
dc.subjectFactor estimulante de colonias de granulocitosspa
dc.subjectProtozoariospa
dc.subjectFactor estimulante de colonias de granulocitos y macrófagosspa
dc.subjectAgente bloqueador del receptor de interleucina 1spa
dc.subjectInterleucina 1spa
dc.subjectInterleucina 13spa
dc.subjectInterleucina 15spa
dc.subjectInterleucina 17spa
dc.subjectInterleucina 1Betaspa
dc.subjectInterleucina 2spa
dc.subjectInterleucina 4spa
dc.subjectInterleucina 6spa
dc.subjectInterleucina 8spa
dc.subjectProteína inflamatoria de macrófagos 1Betaspa
dc.subjectProteína quimiotáctica de monocitos 1spa
dc.subjectAnticuerpo del parásitospa
dc.subjectFactor de necrosis tumoral alfaspa
dc.subjectHumor acuosospa
dc.subjectArtículo Clínicospa
dc.subjectEstudio controladospa
dc.subjectEstudio transversalspa
dc.subjectEnsayo inmunoabsorbente ligado a enzimasspa
dc.subjectExamen de la vistaspa
dc.subjectTécnica de genotipadospa
dc.subjectInmunotransferenciaspa
dc.subjectReacción en cadena de la polimerasa multiplexspa
dc.subjectCarga Parásitaspa
dc.subjectReconocimiento de patronesspa
dc.subjectExpresión de proteínasspa
dc.subjectReacción en cadena de la polimerasa en tiempo realspa
dc.subjectSerotipificaciónspa
dc.subjectToxoplasmaspa
dc.subjectToxoplasmosisspa
dc.subjectADNspa
dc.subjectGenotipospa
dc.subjectInterferon-Gammaspa
dc.subjectTipificación de secuencias multilocusspa
dc.subjectReacción en cadena de la polimerasaspa
dc.subject.keywordSerotypingspa
dc.subject.keywordGenotypespa
dc.subject.keywordDnaspa
dc.subject.keywordMultilocus Sequence Typingeng
dc.subject.keywordPolymerase Chain Reactioneng
dc.subject.keywordMiddle Agedspa
dc.subject.keywordReal Time Polymerase Chain Reactioneng
dc.subject.keywordProtein Expressioneng
dc.subject.keywordPattern Recognitioneng
dc.subject.keywordParasite Loadeng
dc.subject.keywordMultiplex Polymerase Chain Reactioneng
dc.subject.keywordGenotyping Techniqueeng
dc.subject.keywordImmunoblottingeng
dc.subject.keywordEye Examinationeng
dc.subject.keywordEnzyme Linked Immunosorbent Assayeng
dc.subject.keywordCross-Sectional Studyeng
dc.subject.keywordClinical Articleeng
dc.subject.keywordControlled Studyeng
dc.subject.keywordAqueous Humoreng
dc.subject.keywordTumor Necrosis Factor Alphaeng
dc.subject.keywordParasite Antibodyeng
dc.subject.keywordMonocyte Chemotactic Protein 1eng
dc.subject.keywordInterleukin 8eng
dc.subject.keywordMacrophage Inflammatory Protein 1Betaeng
dc.subject.keywordInterleukin 6eng
dc.subject.keywordInterleukin 4eng
dc.subject.keywordInterleukin 2eng
dc.subject.keywordInterleukin 17eng
dc.subject.keywordInterleukin 1Betaeng
dc.subject.keywordInterleukin 15eng
dc.subject.keywordInterleukin 13eng
dc.subject.keywordInterleukin 1eng
dc.subject.keywordInterleukin 1 Receptor Blocking Agenteng
dc.subject.keywordStimulating Factoreng
dc.subject.keywordGranulocyte Macrophage Colonyeng
dc.subject.keywordGamma Interferoneng
dc.subject.keywordProtozoaneng
dc.subject.keywordGranulocyte Colony Stimulating Factoreng
dc.subject.lembToxoplasmosisspa
dc.subject.lembCitocinasspa
dc.subject.lembQuimiocinasspa
dc.titleSevere South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levelsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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