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Evaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombia

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dc.creatorHerrera, Giovannyspa
dc.creatorCastillo, Adrianaspa
dc.creatorAyala, Martha S.spa
dc.creatorFlórez, Carolinaspa
dc.creatorCantillo-Barraza, Omarspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-26T00:03:10Z
dc.date.available2020-05-26T00:03:10Z
dc.date.created2019spa
dc.description.abstractBackground: Leishmaniasis caused by different species of Leishmania affect 98 countries worldwide. Visceral Leishmaniasis (VL) is the mortal clinical presentation of the disease that causes the dead to more than 90% of the patients who suffer it. The diagnosis of VL is made by the direct observation of the parasite in bone marrow, spleen and/or liver aspirates that requires complex proceedings. Therefore, serum samples are submitted to Indirect Immunofluorescence to identify the presence of anti-Leishmania antibodies. Despite the variability in the diagnostic performance of the Immunochromatographic Tests (ICTs), there are many evidences that suggest that ICTs can be used for epidemiological screening. However, in Colombia there are not any evidence about the performance of the ICTs for VL diagnosis, both for human and canine serum samples. Therefore, this study evaluated the diagnostic performance of 4 ICTs for VL (2 ICTs in human sera and 2 ICTs in canine sera) in samples from endemic areas of Colombia. Methods: We selected a total of 156 human serum samples (82 positive and 74 negative for VL) and 126 canine serum samples (71 positive and 54 negative) diagnosed by in house Indirect Immunofluorescence (IIF). The samples were submitted to the ICTs following the manufacturers' instructions. Statistical analysis was performed to evaluate the diagnostic performance of each ICT in comparison with the IIF. PCR for HSP70 gene and sanger sequencing was performed in samples with negative results for both ICTs. Results: The sensitivity (S) of both ICTs for human samples (Ad-bio Leishmania IgG/IgM Combo Rapid Test and Kalazar Detect™) was 91.5% and specificity (E) were 93.2 and 89.2% respectively, while for the ICTs tested on canine samples (Kalazar Detect™ Rapid Test, Canine and DPP® CVL rapid test) we found S values between 82.9 and 85.7% and E values between 79.6 and 92.6%. We found L. infantum by PCR and sequencing in 2 human samples, and L. braziliensis and L. amazonensis in canine serum samples that were negative by both ICTs. Conclusions: We conclude that both tests evaluated on human samples have a similar diagnostic performance, while the Kalazar Detect™ Rapid Test, Canine showed a better diagnostic performance than the DPP® CVL rapid test evaluated on canine samples. Also, we suggest that it is necessary to design tests with antigens of the circulating strains to increase its diagnostic utility. © 2019 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s12879-019-4353-0
dc.identifier.issn14712334
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23567
dc.language.isoengspa
dc.publisherBioMed Central Ltd.spa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleBMC Infectious Diseases
dc.relation.citationVolumeVol. 19
dc.relation.ispartofBMC Infectious Diseases, ISSN:14712334, Vol.19, No.1 (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85071622190&doi=10.1186%2fs12879-019-4353-0&partnerID=40&md5=0f040c24d96234ebd8580810a0baea9cspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordHeat shock protein 70spa
dc.subject.keywordArticlespa
dc.subject.keywordClinical effectivenessspa
dc.subject.keywordColombiaspa
dc.subject.keywordDog breedspa
dc.subject.keywordHumanspa
dc.subject.keywordImmunofluorescencespa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPredictive valuespa
dc.subject.keywordReceiver operating characteristicspa
dc.subject.keywordSanger sequencingspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordVisceral leishmaniasisspa
dc.subject.keywordAnimalspa
dc.subject.keywordDiagnostic testspa
dc.subject.keywordDogspa
dc.subject.keywordDog diseasespa
dc.subject.keywordEvaluation studyspa
dc.subject.keywordGeneticsspa
dc.subject.keywordImmunoassayspa
dc.subject.keywordImmunologyspa
dc.subject.keywordIndirect fluorescent antibody techniquespa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordLeishmania infantumspa
dc.subject.keywordParasitologyspa
dc.subject.keywordProceduresspa
dc.subject.keywordVeterinary medicinespa
dc.subject.keywordVisceral leishmaniasisspa
dc.subject.keywordAnimalsspa
dc.subject.keywordColombiaspa
dc.subject.keywordDiagnostic testseng
dc.subject.keywordDog diseasesspa
dc.subject.keywordDogsspa
dc.subject.keywordFluorescent antibody techniqueeng
dc.subject.keywordHumansspa
dc.subject.keywordImmunoassayspa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordLeishmania infantumspa
dc.subject.keywordLeishmaniasiseng
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordImmunochromatographic testspa
dc.subject.keywordIndirect immunofluorescencespa
dc.subject.keywordPcrspa
dc.subject.keywordSensitivityspa
dc.subject.keywordSpecificityspa
dc.subject.keywordVisceral leishmaniasisspa
dc.titleEvaluation of four rapid diagnostic tests for canine and human visceral Leishmaniasis in Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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