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Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum

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dc.creatorMuñoz, Marinaspa
dc.creatorRestrepo-Montoya, Danielspa
dc.creatorKumar, Nitinspa
dc.creatorIraola, Gregoriospa
dc.creatorHerrera, Giovannyspa
dc.creatorRíos-Chaparro, Dora Ispa
dc.creatorDíaz Arévalo, Diana
dc.creatorPatarroyo, Manuel A.
dc.creatorLawley, Trevor Dspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-26T00:02:21Z
dc.date.available2020-05-26T00:02:21Z
dc.date.created2019spa
dc.description.abstractSome well-known Clostridiales species such as Clostridium difficile and C. perfringens are agents of high impact diseases worldwide. Nevertheless, other foreseen Clostridiales species have recently emerged such as Clostridium tertium and C. paraputrificum. Three fecal isolates were identified as Clostridium tertium (Gcol.A2 and Gcol.A43) and C. paraputrificum (Gcol.A11) during public health screening for C. difficile infections in Colombia. C. paraputrificum genomes were highly diverse and contained large numbers of accessory genes. Genetic diversity and accessory gene percentage were lower among the C. tertium genomes than in the C. paraputrificum genomes. C. difficile tcdA and tcdB toxins encoding homologous sequences and other potential virulence factors were also identified. EndoA interferase, a toxic component of the type II toxin-antitoxin system, was found among the C. tertium genomes. toxA was the only toxin encoding gene detected in Gcol.A43, the Colombian isolate with an experimentally-determined high cytotoxic effect. Gcol.A2 and Gcol.A43 had higher sporulation efficiencies than Gcol.A11 (84.5%, 83.8% and 57.0%, respectively), as supported by the greater number of proteins associated with sporulation pathways in the C. tertium genomes compared with the C. paraputrificum genomes (33.3 and 28.4 on average, respectively). This work allowed complete genome description of two clostridiales species revealing high levels of intra-taxa diversity, accessory genomes containing virulence-factors encoding genes (especially in C. paraputrificum), with proteins involved in sporulation processes more highly represented in C. tertium. These finding suggest the need to advance in the study of those species with potential importance at public health level. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor and Francis Group.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1080/21505594.2019.1637699
dc.identifier.issn21505594
dc.identifier.issn21505608
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23475
dc.language.isoengspa
dc.publisherTaylor and Francis Inc.spa
dc.relation.citationEndPage676
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage657
dc.relation.citationTitleVirulence
dc.relation.citationVolumeVol. 10
dc.relation.ispartofVirulence, ISSN:21505594, 21505608, Vol.10, No.1 (2019); pp. 657-676spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85069489063&doi=10.1080%2f21505594.2019.1637699&partnerID=40&md5=eca91a96cd70e58c25dbc5cf6eec61cespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAlbendazolespa
dc.subject.keywordBacterial toxinspa
dc.subject.keywordClostridium difficile toxin aspa
dc.subject.keywordClostridium difficile toxin bspa
dc.subject.keywordEndo a interferasespa
dc.subject.keywordErtapenemspa
dc.subject.keywordIvermectinspa
dc.subject.keywordMeropenemspa
dc.subject.keywordMetronidazolespa
dc.subject.keywordRna 16sspa
dc.subject.keywordTeclozanspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordVirulence factorspa
dc.subject.keywordBacterial proteinspa
dc.subject.keywordVirulence factorspa
dc.subject.keywordAdultspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAntibiotic resistancespa
dc.subject.keywordArticlespa
dc.subject.keywordBacterial genomespa
dc.subject.keywordBacterium culturespa
dc.subject.keywordBacterium identificationspa
dc.subject.keywordBacterium isolationspa
dc.subject.keywordBiomassspa
dc.subject.keywordCell viabilityspa
dc.subject.keywordClostridium difficile infectionspa
dc.subject.keywordClostridium paraputrificumspa
dc.subject.keywordClostridium tertiumspa
dc.subject.keywordComparative studyspa
dc.subject.keywordCryopreservationspa
dc.subject.keywordCytotoxicityspa
dc.subject.keywordDna extractionspa
dc.subject.keywordFeces analysisspa
dc.subject.keywordGene ontologyspa
dc.subject.keywordGene sequencespa
dc.subject.keywordGenetic distancespa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordGenome sizespa
dc.subject.keywordGenomicsspa
dc.subject.keywordHealth care facilityspa
dc.subject.keywordHumanspa
dc.subject.keywordMass screeningspa
dc.subject.keywordMatrix assisted laser desorption ionization time of flight mass spectrometryspa
dc.subject.keywordMetagenomicsspa
dc.subject.keywordNonhumanspa
dc.subject.keywordOptical densityspa
dc.subject.keywordPeptoclostridium difficilespa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPublic healthspa
dc.subject.keywordSequence alignmentspa
dc.subject.keywordSporogenesisspa
dc.subject.keywordTaxonomyspa
dc.subject.keywordToxin-antitoxin systemspa
dc.subject.keywordWhole genome sequencingspa
dc.subject.keywordClostridioides difficilespa
dc.subject.keywordClostridium infectionspa
dc.subject.keywordClostridium tertiumspa
dc.subject.keywordColombiaspa
dc.subject.keywordComparative studyspa
dc.subject.keywordGenetic variationspa
dc.subject.keywordGeneticsspa
dc.subject.keywordMicrobiologyspa
dc.subject.keywordBacterial proteinsspa
dc.subject.keywordClostridium difficilespa
dc.subject.keywordClostridium infectionsspa
dc.subject.keywordClostridium tertiumspa
dc.subject.keywordColombiaspa
dc.subject.keywordGenetic variationspa
dc.subject.keywordGenomeeng
dc.subject.keywordGenomicsspa
dc.subject.keywordHumansspa
dc.subject.keywordVirulence factorsspa
dc.subject.keywordClostridial speciesspa
dc.subject.keywordClostridium paraputrificumspa
dc.subject.keywordClostridium tertiumspa
dc.subject.keywordGenetic diversityspa
dc.subject.keywordVirulence factorsspa
dc.titleComparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificumspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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