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Molecular diagnosis and genotype analysis of Giardia duodenalis in asymptomatic children from a rural area in central Colombia

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dc.creatorRamírez, Juan David
dc.creatorHeredia, Rubén Daríospa
dc.creatorGalindo Hernández, Carolinaspa
dc.creatorLeón, Cielo M.spa
dc.creatorMoncada, Ligia Inésspa
dc.creatorReyes, Patriciaspa
dc.creatorPinilla, Análida Elizabethspa
dc.creatorLopez, Myriam Consuelospa
dc.date.accessioned2020-05-26T00:11:21Z
dc.date.available2020-05-26T00:11:21Z
dc.date.created2015spa
dc.description.abstractGiardiasis is a parasitic infection that affects around 200 million people worldwide. This parasite presents a remarkable genetic variability observed in 8 genetic clusters named as 'assemblages' (A-H). These assemblages are host restricted and could be zoonotic where A and B infect humans and animals around the globe. The knowledge of the molecular epidemiology of human giardiasis in South-America is scarce and also the usefulness of PCR to detect this pathogen in fecal samples remains controversial. The aim of this study was to conduct a cross-sectional study to compare the molecular targets employed for the molecular diagnosis of Giardia DNA and to discriminate the parasite assemblages circulating in the studied population. We analyzed 181 fecal samples from Children at La Virgen, Cundinamarca, Colombia that were DNA-extracted and analyzed by SSU rDNA, tpi and gdh loci. We observed positivity by microscopy of 13% and by PCR around 76-80% depending on the molecular marker. Additionally, a lack of statistical concordance between microscopy and PCR was detected. Regarding the genetic assemblages, we detected assemblage A (3%), assemblage B (90%) and mixed infections assemblages A. +. B (7%). Hence, the sub-assemblages were typed as AI, AII, BIII and BIV across the population. This study represents a reliable attempt to understand the molecular epidemiology of giardiasis in Colombia and the use of PCR to detect cryptic infections. The epidemiological implications are herein discussed. © 2015 Elsevier B.V.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.meegid.2015.03.015
dc.identifier.issn15671348
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24297
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage213
dc.relation.citationStartPage208
dc.relation.citationTitleInfection, Genetics and Evolution
dc.relation.citationVolumeVol. 32
dc.relation.ispartofInfection, Genetics and Evolution, ISSN:15671348, Vol.32,(2015); pp. 208-213spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84925678851&doi=10.1016%2fj.meegid.2015.03.015&partnerID=40&md5=620c7d94b0530391340e43b77d8370d0spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordHelminth DNAspa
dc.subject.keywordRibosome DNAspa
dc.subject.keywordProtozoaneng
dc.subject.keywordDNAeng
dc.subject.keywordProtozoal DNAspa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordAdolescentspa
dc.subject.keywordArticlespa
dc.subject.keywordAsymptomatic infectionspa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCross-sectional studyspa
dc.subject.keywordDiagnostic test accuracy studyspa
dc.subject.keywordDNA sequencespa
dc.subject.keywordFeces analysisspa
dc.subject.keywordGdh genespa
dc.subject.keywordGenespa
dc.subject.keywordGene locusspa
dc.subject.keywordGenotypespa
dc.subject.keywordGiardia intestinalisspa
dc.subject.keywordGiardiasisspa
dc.subject.keywordHumanspa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMicroscopyspa
dc.subject.keywordMolecular diagnosisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordParasite prevalencespa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPriority journalspa
dc.subject.keywordRural areaspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordTpi genespa
dc.subject.keywordChildspa
dc.subject.keywordFecesspa
dc.subject.keywordGenetic variationspa
dc.subject.keywordGeneticsspa
dc.subject.keywordGenotypespa
dc.subject.keywordGenotyping techniquespa
dc.subject.keywordGiardia intestinalisspa
dc.subject.keywordGiardiasisspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMolecular epidemiologyspa
dc.subject.keywordParasitologyspa
dc.subject.keywordRural populationspa
dc.subject.keywordAnimaliaspa
dc.subject.keywordGiardiaspa
dc.subject.keywordGiardia intestinalisspa
dc.subject.keywordAsymptomatic Infectionsspa
dc.subject.keywordChildspa
dc.subject.keywordColombiaspa
dc.subject.keywordCross-Sectional Studiesspa
dc.subject.keywordDNAeng
dc.subject.keywordFecesspa
dc.subject.keywordGenetic Variationspa
dc.subject.keywordGenotypespa
dc.subject.keywordGenotyping Techniquesspa
dc.subject.keywordGiardia lambliaspa
dc.subject.keywordGiardiasisspa
dc.subject.keywordHumansspa
dc.subject.keywordMolecular Epidemiologyspa
dc.subject.keywordPolymerase Chain Reactionspa
dc.subject.keywordProtozoan Proteinsspa
dc.subject.keywordRural Populationspa
dc.subject.keywordSequence Analysiseng
dc.subject.keywordAssemblagesspa
dc.subject.keywordDiagnosisspa
dc.subject.keywordGenotypesspa
dc.subject.keywordGiardiasisspa
dc.subject.keywordMolecular epidemiologyspa
dc.subject.keywordPCRspa
dc.titleMolecular diagnosis and genotype analysis of Giardia duodenalis in asymptomatic children from a rural area in central Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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