Ítem
Solo Metadatos
Evaluation of the multispecies coalescent method to explore intra-Trypanosoma cruzi i relationships and genetic diversity
Título de la revista
Autores
Gómez-Hernández C.
Pérez S.D.
Rezende-Oliveira K.
Barbosa C.G.
Lages-Silva E.
Ramírez L.E.
Ramírez, Juan David
Fecha
2019
Directores
ISSN de la revista
Título del volumen
Editor
Cambridge University Press
Buscar en:
Métricas alternativas
Resumen
Abstract
Chagas Disease is a zoonosis caused by the parasite Trypanosoma cruzi. Several high-resolution markers have subdivided T. cruzi taxon into at least seven lineages or Discrete Typing Units (DTUs) (TcI-TcVI and TcBat). Trypanosoma cruzi I is the most diverse and geographically widespread DTU. Recently a TcI genotype related to domestic cycles was proposed and named as TcIDOM. Herein, we combined traditional markers and housekeeping genes and applied a Multispecies Coalescent method to explore intra-TcI relationships, lineage boundaries and genetic diversity in a random set of isolates and DNA sequences retrieved from Genbank from different countries in the Americas. We found further evidence supporting TcIDOM as an independent and emerging genotype of TcI at least in Colombia and Venezuela. We also found evidence of high phylogenetic incongruence between parasite's gene trees (including introgression) and embedded species trees, and a lack of genetic structure among geography and hosts, illustrating the complex dynamics and epidemiology of TcI across the Americas. These findings provide novel insights into T. cruzi systematics and epidemiology and support the need to assess parasite diversity and lineage boundaries through hypothesis testing using different approaches to those traditionally employed, including the Bayesian Multispecies coalescent method. Copyright © Cambridge University Press 2019.
Palabras clave
Keywords
Genomic DNA , Molecular marker , Article , Chagas disease , Cladistics , Gene structure , Genetic variability , Housekeeping gene , Nonhuman , Parasite cultivation , Parasite isolation , Priority journal , Trypanosoma cruzi , Chagas disease , Discrete typing unit , Genotype , Housekeeping genes , Lineage , Multispecies coalescent
