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Toxicity of potential fungal defense proteins towards the fungivorous nematodes Aphelenchus avenae and Bursaphelenchus okinawaensis

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dc.creatorTayyrov, Annageldispa
dc.creatorSchmieder, Stefanie S.spa
dc.creatorBleuler-Martinez, Silviaspa
dc.creatorPlaza, David F.spa
dc.creatorKünzler, Markusspa
dc.date.accessioned2020-05-25T23:57:00Z
dc.date.available2020-05-25T23:57:00Z
dc.date.created2018spa
dc.description.abstractResistance of fungi to predation is thought to be mediated by toxic metabolites and proteins. Many of these fungal defense effectors are highly abundant in the fruiting body and not produced in the vegetative mycelium. The defense function of fruiting body-specific proteins, however, including cytoplasmically localized lectins and antinutritional proteins such as biotin-binding proteins, is mainly based on toxicity assays using bacteria as a heterologous expression system, with bacterivorous/omnivorous model organisms as predators. Here, we present an ecologically more relevant experimental setup to assess the toxicity of potential fungal defense proteins towards the fungivorous, stylet-feeding nematodes Aphelenchus avenae and Bursaphelenchus okinawaensis. As a heterologous expression host, we exploited the filamentous fungus Ashbya gossypii. Using this new system, we assessed the toxicity of six previously characterized, cytoplasmically localized, potential defense proteins from fruiting bodies of different fungal phyla against the two fungivorous nematodes. We found that all of the tested proteins were toxic against both nematodes, albeit to various degrees. The toxicity of these proteins against both fungivorous and bacterivorous nematodes suggests that their targets have been conserved between the different feeding groups of nematodes and that bacterivorous nematodes are valid model organisms to assess the nematotoxicity of potential fungal defense proteins. © 2018 American Society for Microbiology.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1128/AEM.02051-18
dc.identifier.issn00992240
dc.identifier.issn10985336
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22578
dc.language.isoengspa
dc.publisherAmerican Society for Microbiologyspa
dc.relation.citationIssueNo. 23
dc.relation.citationTitleApplied and Environmental Microbiology
dc.relation.citationVolumeVol. 84
dc.relation.ispartofApplied and Environmental Microbiology, ISSN:00992240, 10985336, Vol.84, No.23 (2018)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85056623341&doi=10.1128%2fAEM.02051-18&partnerID=40&md5=2b4a05c693e5f11e6ae23200dcc4bda8spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordFungispa
dc.subject.keywordFungaleng
dc.subject.keywordToxicityspa
dc.subject.keywordAshbya gossypiispa
dc.subject.keywordAvidinspa
dc.subject.keywordFilamentous fungispa
dc.subject.keywordLectinspa
dc.subject.keywordMycophagyspa
dc.subject.keywordNematotoxicityspa
dc.subject.keywordProteinsspa
dc.subject.keywordDefense mechanismspa
dc.subject.keywordExperimental designspa
dc.subject.keywordFrugivoryspa
dc.subject.keywordFungusspa
dc.subject.keywordHostspa
dc.subject.keywordMycophagyspa
dc.subject.keywordNematodespa
dc.subject.keywordProteinspa
dc.subject.keywordToxicityspa
dc.subject.keywordAphelenchus avenaespa
dc.subject.keywordBursaphelenchusspa
dc.subject.keywordEremothecium gossypiispa
dc.subject.keywordFungispa
dc.subject.keywordNematodaspa
dc.subject.keywordFungal proteinspa
dc.subject.keywordAnimalspa
dc.subject.keywordChemistryspa
dc.subject.keywordDrug effectspa
dc.subject.keywordFeeding behaviorspa
dc.subject.keywordFruiting bodyspa
dc.subject.keywordFungusspa
dc.subject.keywordPhysiologyspa
dc.subject.keywordTylenchidaspa
dc.subject.keywordAnimalsspa
dc.subject.keywordFeeding Behaviorspa
dc.subject.keywordFruiting Bodieseng
dc.subject.keywordFungal Proteinsspa
dc.subject.keywordFungispa
dc.subject.keywordTylenchidaspa
dc.subject.keywordAshbya gossypiispa
dc.subject.keywordAvidinspa
dc.subject.keywordFilamentous fungusspa
dc.subject.keywordLectinspa
dc.subject.keywordMycophagyspa
dc.subject.keywordNematotoxicityspa
dc.titleToxicity of potential fungal defense proteins towards the fungivorous nematodes Aphelenchus avenae and Bursaphelenchus okinawaensisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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