Ítem
Solo Metadatos

DNA demethylation agent 5azadC downregulates HPV16 E6 expression in cervical cancer cell lines independently of TBX2 expression

Mostrar el registro sencillo de la publicación
dc.creatorPerrard, Jeromespa
dc.creatorMorel, Adrien
dc.creatorMeznad, Koceilaspa
dc.creatorPaget-Bailly, Philippespa
dc.creatorDalstein, Veroniquespa
dc.creatorGuenat, Davidspa
dc.creatorMourareau, Celinespa
dc.creatorClavel, Christinespa
dc.creatorFauconnet, Sylviespa
dc.creatorBaguet, Aureliespa
dc.creatorMougin, Christianespa
dc.creatorPretet, Jean-Lucspa
dc.date.accessioned2020-05-25T23:57:41Z
dc.date.available2020-05-25T23:57:41Z
dc.date.created2020spa
dc.description.abstractHPV16 is the most carcinogenic human papillomavirus and causes >50% of cervical cancers, the majority of anal cancers and 30% of oropharyngeal squamous cell carcinomas. HPV carcinogenesis relies on the continuous expression of the two main viral oncoproteins E6 and E7 that target >150 cellular proteins. Among them, epigenetic modifiers, including DNA Methyl Transferases (DNMT), are dysregulated, promoting an aberrant methylation pattern in HPV-positive cancer cells. It has been previously reported that the treatment of HPV-positive cervical cancer cells with DNMT inhibitor 5-aza-2'-deoxycytidine (5azadC) caused the downregulation of E6 expression due to mRNA destabilization that was mediated by miR-375. Recently, the T-box transcription factor 2 (TBX2) has been demonstrated to repress HPV LCR activity. In the current study, the role of TBX2 in E6 repression was investigated in HPV16 cervical cancer cell lines following 5azadC treatment. A decrease of E6 expression was accompanied by p53 and p21 restoration. While TBX2 mRNA was upregulated in 5azadC-treated SiHa and Ca Ski cells, TBX2 protein was not detectable. Furthermore, the overexpression of TBX2 protein in cervical cancer cells did not allow the repression of E6 expression. The TBX2 transcription factor is therefore unlikely to be associated with the repression of E6 following 5azadC treatment of SiHa and Ca Ski cells. © 2020 Spandidos Publications. All rights reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3892/ol.2019.11158
dc.identifier.issn17921082
dc.identifier.issn17921074
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22720
dc.language.isoengspa
dc.publisherSpandidos Publicationsspa
dc.relation.citationEndPage1081
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage1074
dc.relation.citationTitleOncology Letters
dc.relation.citationVolumeVol. 19
dc.relation.ispartofOncology Letters, ISSN:17921082, 17921074, Vol.19, No.1 (2020); pp. 1074-1081spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85077284303&doi=10.3892%2fol.2019.11158&partnerID=40&md5=f21482072597718b8c52232ad6a0a381spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordDecitabinespa
dc.subject.keywordMethyltransferasespa
dc.subject.keywordMicrorna 375spa
dc.subject.keywordProtein e6spa
dc.subject.keywordProtein e7spa
dc.subject.keywordProtein p21spa
dc.subject.keywordProtein p53spa
dc.subject.keywordSmall interfering rnaspa
dc.subject.keywordT box transcription factorspa
dc.subject.keywordArticlespa
dc.subject.keywordCa ski cell linespa
dc.subject.keywordCarcinogenesisspa
dc.subject.keywordCervical cancer cell linespa
dc.subject.keywordChemoluminescencespa
dc.subject.keywordControlled studyspa
dc.subject.keywordDna demethylationspa
dc.subject.keywordDown regulationspa
dc.subject.keywordGene overexpressionspa
dc.subject.keywordGene repressionspa
dc.subject.keywordGenetic transfectionspa
dc.subject.keywordHela cell linespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordHuman papillomavirus 16 infectionspa
dc.subject.keywordHuman papillomavirus type 16spa
dc.subject.keywordImmune dysregulationspa
dc.subject.keywordImmunoblottingspa
dc.subject.keywordImmunoprecipitationspa
dc.subject.keywordLuciferase assayspa
dc.subject.keywordMcf-7 cell linespa
dc.subject.keywordMrna expression levelspa
dc.subject.keywordPapillomavirus infectionspa
dc.subject.keywordProtein expressionspa
dc.subject.keywordProtein expression levelspa
dc.subject.keywordReverse transcription polymerase chain reactionspa
dc.subject.keywordRna extractionspa
dc.subject.keywordSiha cell linespa
dc.subject.keywordUpregulationspa
dc.subject.keywordUterine cervix cancerspa
dc.subject.keywordWestern blottingspa
dc.subject.keyword5-aza-2'-deoxycytidinespa
dc.subject.keywordDna methyltransferase inhibitorspa
dc.subject.keywordEpigeneticspa
dc.subject.keywordHuman papillomavirus-induced cancerspa
dc.subject.keywordT-boxspa
dc.titleDNA demethylation agent 5azadC downregulates HPV16 E6 expression in cervical cancer cell lines independently of TBX2 expressionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Colecciones
Artículos