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Efecto de la hipoxia-reoxigenación y las radiaciones ionizantes en la captación de glucosa en líneas tumorales de seno y colon cocultivadas con células endoteliales.

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dc.contributor.advisorOndo Méndez, Alejandro Oyono
dc.creatorAgudelo Ramírez, Adriana
dc.creator.degreeMagíster en Ciencias con Énfasis en Genética Humana
dc.date.accessioned2013-02-27T17:04:33Z
dc.date.available2013-02-27T17:04:33Z
dc.date.created2012-11-22
dc.date.issued2012
dc.descriptionLa captación de glucosa y su conversión en lactato juega un papel fundamental en el metabolismo tumoral, independientemente de la concentración de oxígeno presente en el tejido (efecto Warburg). Sin embrago, dicha captación varía de un tipo tumoral a otro, y dentro del mismo tumor, situación que podría depender de las características microambientales tumorales (fluctuaciones de oxígeno, presencia de otros tipos celulares) y de factores estresores asociados a los tratamientos. Se estudió el efecto de la hipoxia-reoxigenación (HR) y las radiaciones ionizantes (RI) sobre la captación de glucosa, en cultivos de líneas tumorales MCF-7 y HT-29, cultivadas de forma aislada o en cocultivo con la línea celular EAhy296. Se encontró que la captación de glucosa en HR es diferente para lo descrito en condiciones de hipoxia permanente y que es modificada en el cocultivo. Se identificaron poblaciones celulares dentro de la misma línea celular, de alta y baja captación de glucosa, lo que implicaría una simbiosis metabólica de la célula como respuesta adaptativa a las condiciones tumorales. Se evaluó la expresión de NRF2 y la translocación nuclear de NRF2 y HIF1a, como vías de respuesta a estrés celular e hipoxia. La translocación nuclear de las proteínas evaluadas explicaría el comportamiento metabólico de las células tumorales de seno, pero no de colon, por lo cual deben existir otras vías metabólicas implicadas. Las diferencias en el comportamiento de las células tumorales en HR en relación con hipoxia permitirá realizar planeaciones dosimétricas más dinámicas, que reevalúen las condiciones de oxigenación tumoral constantemente.spa
dc.description.abstractGlucose uptake and it´s conversion to lactate plays an important role for tumor metabolism. This phenomena does not depend on oxygen present at the tissue (Warburg effect). This glucose uptake is different between tumors and even into the same tumor. This observation may be depending on tumor microenviroment (fluctuations on oxygen availability and the presence of other non tumoral cell types) and other stress factors associated to the treatments for the disease. We evaluate the effect of hypoxia-reoxigenation (HR) and ionizing radiations (IR) on the glucose uptake, in cell cultures of MCF-7 and HT29 in isolated cultures or cocultured with EAhy926. We found that glucose uptake for the cells exposed to HR were different from that described for the hypoxia conditions. Also we observed that this pattern were modified when tumor cells were cocultured with the endothelial cell line. We identified cell populations in relation to glucose uptake (High, medium, low). We evaluate the nuclear translocation of NRF2 and HIF1a proteins, as an evaluation of hypoxia and cellular stress pathways, finding a possible correlation with breast cancer cells, but no with the colon cell line. This suggest that it may be another pathways involved. The different patterns of glucose uptake and metabolism of tumor cells in relation with glucose uptake, when comparing hypoxia and hypoxia-reoxigenation, could lead to a better dossymmetric planning taking account the variations on oxygen concentration into the tumor. eng
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dc.identifier.doihttps://doi.org/10.48713/10336_4278
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/4278
dc.language.isospa
dc.publisherUniversidad del Rosariospa
dc.publisher.departmentFacultad de medicinaspa
dc.publisher.programMaestría en Ciencias con Énfasis en Genética Humanaspa
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dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectHipoxia-reoxigenaciónspa
dc.subjectNRF2spa
dc.subjectRadiaciones ionizantesspa
dc.subjectCáncer de seno y colonspa
dc.subjectCocultivospa
dc.subjectHIF1aspa
dc.subjectSimbiosis metabólicaspa
dc.subject.keywordHypoxia-reoxigenationeng
dc.subject.keywordWarburg effecteng
dc.subject.keywordIonizing radiationeng
dc.subject.keywordBreast and colon cancereng
dc.subject.keywordCocultureeng
dc.subject.keywordHIF1a and NRF2eng
dc.subject.keywordSymbiosiseng
dc.subject.lembCáncerspa
dc.subject.lembCultivo de célulasspa
dc.subject.lembFactor 2 relacionado con nfspa
dc.subject.lembHipoxiaspa
dc.subject.lembNeoplasmas de la mamaspa
dc.subject.lembNeoplasmas del colonspa
dc.subject.lembTécnicas de cocultivospa
dc.titleEfecto de la hipoxia-reoxigenación y las radiaciones ionizantes en la captación de glucosa en líneas tumorales de seno y colon cocultivadas con células endoteliales.spa
dc.typemasterThesiseng
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTesis de maestríaspa
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