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Citotoxicidad y genotoxicidad in vitro del clorhidrato de cocaína en la línea celular de fibrosarcoma humano HT1080

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dc.contributor.advisorGroot de Restrepo, Helena
dc.creatorRojas Martínez, Maurix Fernando
dc.creator.degreeEspecialista en Toxicología Clínicaspa
dc.creator.degreetypeFull timespa
dc.date.accessioned2020-04-13T15:24:47Z
dc.date.available2020-04-13T15:24:47Z
dc.date.created2004
dc.date.issued2004
dc.descriptionEl clorhidrato de cocaína es una sal hidrosoluble con efectos anestésicos y estimulantes sobre el Sistema Nervioso Central. Presenta efectos adversos a nivel cardiovascular, pulmonar y nervioso. A nivel celular, gracias a sus propiedades químicas y su degradación metabólica (vía microsomal oxidativa) induce la producción de radicales libres que pueden interactuar directamente con el material genético o producir alteraciones en la fisiología celular. El objetivo de este trabajo fue determinar la citotoxicidad crónica, aguda y la genotoxicidad del clorhidrato de cocaína en células de fibrosarcoma humano HT1080. Para tal fin la citotoxicidad crónica y aguda se determinaron mediante el colorante cristal violeta y azul de trypan respectivamente, después de exponer las células a diferentes dosis del compuesto. La genotoxicidad se determino mediante el ensayo del cometa. Los datos se analizaron utilizando la prueba de Dunnet y correlación de Pearson. En los resultados se observa un efecto citotóxico dosis dependiente (porcentaje de viabilidad celular y concentración de cocaína) en dosis superiores a 3000 μM después de 72 horas de exposición (P< 0.05). En la citotoxicidad aguda las células HT1080 expuestas a concentraciones menores a 3.5 mM, presentaron una viabilidad > 70%. Se evidencia daño en el ADN después del tratamiento en concentraciones de 0.5-3.5 mM (p<0.001). En conclusión el clorhidrato de cocaína es genotoxico para la células HT1080 en dosis mayores a 0.5 mM.spa
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.48713/10336_21568
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/21568
dc.language.isospaspa
dc.publisher.departmentEscuela de Medicina y Ciencias de la Saludspa
dc.publisher.programEspecialización en Toxicología Clínicaspa
dc.rights.accesRightsinfo:eu-repo/semantics/closedAccess
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dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subjectCitotoxicidad crónicaspa
dc.subjectCitotoxicidad agudaspa
dc.subjectCocaínaspa
dc.subjectDrogadicciónspa
dc.subject.ddcEnfermedadesspa
dc.subject.lembCitotoxicidad por mediación celularspa
dc.subject.lembDrogadicción::Aspectos Genéticosspa
dc.subject.lembGenéticaspa
dc.subject.lembToxicología genéticaspa
dc.subject.lembToxicologíaspa
dc.titleCitotoxicidad y genotoxicidad in vitro del clorhidrato de cocaína en la línea celular de fibrosarcoma humano HT1080spa
dc.typemasterThesiseng
dc.type.documentAnálisis de casospa
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de gradospa
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