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dc.creatorHernández, Carolina 
dc.creatorCucunubá, Zulma 
dc.creatorFlórez, Carolina 
dc.creatorOlivera, Mario 
dc.creatorValencia-Hernandez, Carlos A. 
dc.creatorZambrano, Pilar 
dc.creatorLeón, Cielo 
dc.creatorRamírez, Juan David 
dc.date.accessioned2019-01-22T20:49:54Z
dc.date.available2019-01-22T20:49:54Z
dc.date.created2016-10-28
dc.date.issued2016
dc.identifier.issnIssn 1935-2727
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18908
dc.descriptionFe de erratas. The fifth author's name is spelled incorrectly. The correct name is: Carlos A. Valencia-Hernandez. The correct citation is: Hernández C, Cucunubá Z, Flórez C, Olivera M, Valencia-Hernandez C.A., Zambrano P, et al. (2016) Molecular Diagnosis of Chagas Disease in Colombia: Parasitic Loads and Discrete Typing Units in Patients from Acute and Chronic Phases. PLoS Negl Trop Dis 10(9): e0004997. doi: 10.1371/journal.pntd.0004997
dc.description.abstractBackground: The diagnosis of Chagas disease is complex due to the dynamics of parasitemia in the clinical phases of the disease. The molecular tests have been considered promissory because they detect the parasite in all clinical phases. Trypanosoma cruzi presents significant genetic variability and is classified into six Discrete Typing Units TcI-TcVI (DTUs) with the emergence of foreseen genotypes within TcI as TcIDom and TcI Sylvatic. The objective of this study was to determine the operating characteristics of molecular tests (conventional and Real Time PCR) for the detection of T. cruzi DNA, parasitic loads and DTUs in a large cohort of Colombian patients from acute and chronic phases. Methodology/Principal Findings: Samples were obtained from 708 patients in all clinical phases. Standard diagnosis (direct and serological tests) and molecular tests (conventional PCR and quantitative PCR) targeting the nuclear satellite DNA region. The genotyping was performed by PCR using the intergenic region of the mini-exon gene, the 24Sa, 18S and A10 regions. The operating capabilities showed that performance of qPCR was higher compared to cPCR. Likewise, the performance of qPCR was significantly higher in acute phase compared with chronic phase. The median parasitic loads detected were 4.69 and 1.33 parasite equivalents/mL for acute and chronic phases. The main DTU identified was TcI (74.2%). TcIDom genotype was significantly more frequent in chronic phase compared to acute phase (82.1% vs 16.6%). The median parasitic load for TcIDom was significantly higher compared with TcI Sylvatic in chronic phase (2.58 vs.0.75 parasite equivalents/ml). Conclusions/Significance: The molecular tests are a precise tool to complement the standard diagnosis of Chagas disease, specifically in acute phase showing high discriminative power. However, it is necessary to improve the sensitivity of molecular tests in chronic phase. The frequency and parasitemia of TcIDom genotype in chronic patients highlight its possible relationship to the chronicity of the disease. © 2016 Hernández et al.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.relation.ispartofPLoS Neglected Tropical Diseases, ISSN: 1935-2727, Vol. 10/No. 9 (2016)
dc.relation.urihttps://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0005112&type=printable
dc.relation.urihttp://repository.urosario.edu.co/handle/10336/18907
dc.rights.uri
dc.subjectMolecular Marker
dc.subjectProtozoal Dna
dc.subjectSpacer Dna
dc.subjectArea Under The Curve
dc.subjectBlood Culture
dc.subjectBlood Smear
dc.subjectChagas Disease
dc.subjectColombia
dc.subjectDiagnostic Accuracy
dc.subjectDiagnostic Test Accuracy Study
dc.subjectDiscrete Typing Unit
dc.subjectDisease Course
dc.subjectEnzyme Linked Immunosorbent Assay
dc.subjectGenetic Variability
dc.subjectGenotype
dc.subjectHemagglutination Inhibition Test
dc.subjectHuman
dc.subjectImmunofluorescence Test
dc.subjectMale
dc.subjectMicroorganism Detection
dc.subjectMolecular Diagnosis
dc.subjectNonhuman
dc.subjectParasite Load
dc.subjectParasitemia
dc.subjectPredictive Value
dc.subjectReal Time Polymerase Chain Reaction
dc.subjectReceiver Operating Characteristic
dc.subjectSensitivity And Specificity
dc.subjectTrypanosoma Cruzi
dc.subjectAcute Disease
dc.subjectChagas Disease
dc.subjectChronic Disease
dc.subjectComparative Study
dc.subjectExon
dc.subjectGenetic Variation
dc.subjectIsolation And Purification
dc.subjectMolecular Typing
dc.subjectParasite Load
dc.subjectYoung Adult
dc.subjectAcute Disease
dc.subjectChagas Disease
dc.subjectChronic Disease
dc.subjectColombia
dc.subjectDna, Protozoan
dc.subjectExons
dc.subjectGenetic Variation
dc.subjectGenotype
dc.subjectMale
dc.subjectMolecular Typing
dc.subjectParasite Load
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectSensitivity And Specificity
dc.subjectTrypanosoma Cruzi
dc.subjectYoung Adult
dc.subject.ddcEnfermedades 
dc.subject.lembEnfermedad de chagas
dc.subject.lembParasitemia
dc.subject.lembTrypanosoma cruzi
dc.titleCorrection : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases
dc.typearticle
dc.subject.keywordAdult
dc.subject.keywordArticle
dc.subject.keywordFemale
dc.subject.keywordMiddle Aged
dc.subject.keywordAdult
dc.subject.keywordFemale
dc.subject.keywordHumans
dc.subject.keywordMiddle Aged
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.type.spaArtículo
dc.rights.accesoAbierto (Texto Completo)
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.source.bibliographicCitationhttp://www.who.int/wer/2015/wer9006.pdf, WHO. Weekly epidemiological record—Chagas diseases in Latin America: an epidemiological update based on 2010 estimates. 2015 p. 5–13. Available:
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/
dc.creator.googleHernández, Carolina
dc.creator.googleCucunubá, Zulma
dc.creator.googleFlórez, Carolina
dc.creator.googleOlivera, Mario
dc.creator.googleValencia-Hernandez, Carlos A.
dc.creator.googleZambrano, Pilar
dc.creator.googleLeón, Cielo
dc.creator.googleRamírez, Juan David
dc.relation.citationIssueNo. 9
dc.relation.citationTitlePLoS Neglected Tropical Diseases
dc.relation.citationVolumeVol. 10


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