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Efecto de la Peroxidación Catalítica en Fase Húmeda activada por una Arcilla Pilarizada con Al/Fe sobre la viabilidad de Quistes de Giardia Intestinalis en agua superficial del río Pasto

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dc.contributor.advisorGaleano, Luis Alejandro
dc.contributor.advisorRamírez González, Juan David
dc.contributor.gruplacGrupo de Investigaciones Microbiológicas UR (GIMUR)
dc.creatorReina Hidalgo, Ariana
dc.creator.degreeMagíster en Ciencias Naturales
dc.creator.degreeLevelMaestríaspa
dc.creator.degreetypeFull time
dc.date.accessioned2023-06-27T20:37:18Z
dc.date.available2023-06-27T20:37:18Z
dc.date.created2023-05-18
dc.descriptionGiardia intestinalis es un parásito protozoario con distribución global, que infecta amplia gama de hospederos vertebrados. Tiene dos estadíos de vida, los trofozoítos (forma replicativa) y los quistes (forma transmisible e infectiva) que se encuentran en el agua y alimentos contaminados. En este estudio se monitoreó el ARNm de quistes, como respuesta a la desinfección de agua superficial por Peroxidación Catalítica en Fase Húmeda (PCFH) activada por un catalizador de arcilla pilarizada con Al/Fe (Al/Fe-PILC); PCFH es un Proceso de Oxidación Avanzada (POA) evaluado en este estudio para la eliminación de quistes que exhiben alta resistencia a la cloración y otros métodos convencionales de desinfección. Los quistes de G. intestinalis (cepa WB, ensamblaje A) se cultivaron in vitro; se extrajo ARNm (1 x 105 quistes/mL) y se estandarizó un análisis de RT-qPCR para su detección y cuantificación utilizando los marcadores moleculares 18S-ARNr y β-giardina. Los experimentos catalíticos se realizaron en un reactor semi-continuo de 1 L utilizando agua superficial del río Pasto (Colombia) y se doparon con 100 quistes equivalentes Giardia/L teniendo en cuenta los factores experimentales de pH (6,0 y 7,0) y concentración de hierro activo del catalizador sólido (100 y 300 mg/L). Todos los experimentos catalíticos causaron pérdida de viabilidad de quistes de al menos 4 Log (99,99%); además, hasta alrededor del 35 % del Carbono Orgánico Disuelto (COD) y el 30 % del Nitrógeno Total Disuelto (NTD) se mineralizaron usando una dosis baja de peróxido de hidrógeno (0,037 mg H2O2/mg Fe.mg activo COD) en condiciones ambientales de temperatura (11 °C) y presión (73 kPa). El análisis de varianza mostró que la concentración de Fe activo (mg/L) ejerció un efecto significativo sobre la eliminación de quistes de G. intestinalis, la eliminación de COD y la fracción de H2O2 reaccionada (p < 0,05 con 95 % de nivel de confianza). Por su parte, en la optimización estadística de respuesta múltiple se obtuvo un valor de 0,95 para la función Deseabilidad, donde todas las respuestas alcanzaron el óptimo cuando la concentración de Fe activo fue de aproximadamente 80 mg/L y el pH 6,0. El pH no tuvo efecto significativo en los experimentos catalíticos. Este enfoque podría permitir a corto plazo monitorear a bajo costo la presencia de quistes de Giardia en el agua, así como prevenir la propagación de enfermedades infecciosas que son un problema de salud pública al complementar la desinfección convencional del agua con la PCFH en presencia de Al/Fe-PILC.
dc.description.abstractGiardia intestinalis is a protozoan parasite with a global distribution, infecting a wide range of vertebrate hosts. It has two life stages, trophozoites (replicative stage) and cysts (transmissible and infective stage) found in either contaminated food or water. In this study, the mRNA of cysts was monitored, as a response to the disinfection of surface water by Catalytic Wet Peroxide Oxidation (CWPO) activated by an Al/Fe-pillared clay catalyst (Al/Fe-PILC); CWPO is an Advanced Oxidation Process (AOP) assessed in our study for the removal of cysts that exhibit high resistance to chlorination and other standard disinfection methods. Cysts of G. intestinalis (strain WB, assemblage A) were cultured in vitro; the RNA was extracted (1x105 cysts/mL) and a RT-qPCR analysis was standardized for detection and quantification using the molecular markers 18S-rRNA and β-giardin. The catalytic experiments were carried out in a 1 L semicontinuous reactor using surface water from Pasto River (Colombia) that was doped with 100 equivalent Giardia cysts/L considering the experimental factors pH (6.0 - 7.0) and the concentration of active iron in the solid catalyst (100 - 300 mg/L). All the catalytic experiments caused loss of the cyst viability of at least 4 Log (99.99 %); besides, up to around 35 % of Dissolved Organic Carbon (DOC) and 30 % of Dissolved Total Nitrogen (DTN) vanished by using a low dose of hydrogen peroxide (0.037 mg H2O2/mg active Fe.mg DOC) under pretty middle ambient conditions of temperature (11 °C) and pressure (73 kPa). The analysis of variance showed the concentration of active Fe (mg/L) to exhibit a significant effect on the elimination of G. intestinalis cysts, DOC elimination, and fraction of reacted H2O2 (p < 0.05, 95 % confidence level). Meanwhile, in the statistical multiple-response optimization, a value of 0.95 was obtained in the Desirability function, where all the responses reached the optimum when the concentration of active Fe was approximately 80 mg/L and pH 6.3, whereas the pH did not display a significant effect on the catalytic performance. This approach could allow in the short term to monitor at low cost the presence of Giardia cysts in water as well as to prevent the spread of infectious diseases that are of public health concern by complementing conventional water disinfection with Catalytic Wet Peroxide Oxidation in the presence of Al/Fe-PILCs.
dc.format.extent48 pp
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dc.identifier.doihttps://doi.org/10.48713/10336_39894
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/39894
dc.publisherUniversidad del Rosario
dc.publisher.departmentFacultad de Ciencias Naturales
dc.publisher.programMaestría en Ciencias Naturales
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dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectGiardia intestinalis
dc.subjectPeroxidación Catalítica en Fase Húmeda
dc.subjectViabilidad de quistes
dc.subjectRT-qPCR
dc.subjectArcillas pilarizadas con Al/Fe
dc.subject.keywordGiardia intestinalis
dc.subject.keywordCatalytic Wet Peroxide Oxidation
dc.subject.keywordCyst viability
dc.subject.keywordRT-qPCR
dc.subject.keywordAl/Fe-pillared clay
dc.titleEfecto de la Peroxidación Catalítica en Fase Húmeda activada por una Arcilla Pilarizada con Al/Fe sobre la viabilidad de Quistes de Giardia Intestinalis en agua superficial del río Pasto
dc.title.TranslatedTitleEffect of Catalytic Wet Peroxide Oxidation activated by an Al/Fe pillared clay catalyst on the viability of Giardia intestinalis cysts in surface water from the Pasto River
dc.typebachelorThesis
dc.type.documentTrabajo de grado
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de grado
local.department.reportFacultad de Ciencias Naturales
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