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Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

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dc.creatorBermudez, Adrianaspa
dc.creatorPatarroyo, Manuel E.spa
dc.creatorUrquiza, Mauriciospa
dc.creatorCortés, Jimenaspa
dc.creatorSuárez, Jorgespa
dc.date.accessioned2020-08-19T14:40:05Z
dc.date.available2020-08-19T14:40:05Z
dc.date.created2003-09spa
dc.description.abstractSynthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regiones thar could be involuved in parasite invesion. LSA-1 protein peptides 20630 ( 21 INGKIIKNSEKDEIIKSNLRY 40), 20637 (157 KEKLQGQQSDSEQERRAY 173), 20638 (174 KEKLQEQQSDLEQERLA 190) and 20639 (191KEKLQEQQSDLEQERRAY 207) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptides cell binding was saturable. Peptide 20630 bound specifically to 48 kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (21 INGKIIKNSEKDEIIKSNLRY 40) and 20633 (81 DKELTMSNVKNVSQTNFSLY 100) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 “High Activity Binding Peptides” could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/S0196-9781(03)00135-9
dc.identifier.issnISSN: 0196-9781
dc.identifier.issnEISSN: 1873-5169
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26704
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage657
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage647
dc.relation.citationTitlePeptides
dc.relation.citationVolumeVol. 24
dc.relation.ispartofPeptides, ISSN: 0196-9781;EISSN: 1873-5169, Vol.24, No.5 (May 2003); pp. 647-657spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0196978103001359?via%3Dihubspa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourcePeptidesspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordP. falciparumspa
dc.subject.keywordCritical residuesspa
dc.subject.keywordLiver stage antigen (LSA)spa
dc.subject.keywordHigh activity binding peptides (HABPs)spa
dc.subject.keywordPhosphate buffered saline (PBS)spa
dc.subject.keywordRed blood cells (RBC)spa
dc.titlePeptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytesspa
dc.title.alternativePeptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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