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Prevalence and molecular epidemiology of bovine leukemia virus in Colombian cattle

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dc.creatorFigueroa, Adriana Patricia Corredorspa
dc.creatorSalas, Sandraspa
dc.creatorOlaya-Galán, Nury Nathaliaspa
dc.creatorQuintero, Juan Sebastiánspa
dc.creatorFajardo, Álvarospa
dc.creatorSoñora, Martínspa
dc.creatorMoreno, Pilarspa
dc.creatorCristina, Juanspa
dc.creatorSánchez, Alfredospa
dc.creatorTobón, Juliospa
dc.creatorOrtiz, Diegospa
dc.creatorGutiérrez, María Fernandaspa
dc.date.accessioned2020-05-26T00:11:32Z
dc.date.available2020-05-26T00:11:32Z
dc.date.created2020spa
dc.description.abstractBovine leukemia virus (BLV) is one of the five agents considered most significant for cattle. It is important to determine the prevalence and molecular epidemiology of BLV throughout the country in order to gain a more thorough understanding of the current situation of BLV and to reveal the possibility of masked genotypes that the primers used by OIE are unable to identify. Blood samples were collected at random from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. A total of 62% of the cows present at 92% of the farms were BLV-positive for gag fragment. Genotype 1 was exclusively detected by env gene segment when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. This report is one of the first molecular and epidemiological studies of BLV conducted in Colombia, which contributes to the global epidemiology of the virus; it also highlights the substantial impact of BLV on the country's livestock and thus is a useful resource for farmers and government entities. © 2020 The Authorseng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.meegid.2020.104171
dc.identifier.issn15671348
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24309
dc.language.isoengspa
dc.publisherElsevier B.V.spa
dc.relation.citationTitleInfection, Genetics and Evolution
dc.relation.citationVolumeVol. 80
dc.relation.ispartofInfection, Genetics and Evolution, ISSN:15671348, Vol.80,(2020)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85078044957&doi=10.1016%2fj.meegid.2020.104171&partnerID=40&md5=aaf47933b43a4e75f6587d5722d7e3despa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAgricultural landspa
dc.subject.keywordAgricultural workerspa
dc.subject.keywordAmpliconspa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAnimal tissuespa
dc.subject.keywordArticlespa
dc.subject.keywordBovine leukemia virusspa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordCowspa
dc.subject.keywordEnvelope genespa
dc.subject.keywordFemalespa
dc.subject.keywordGene frequencyspa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordGovernmentspa
dc.subject.keywordLivestockspa
dc.subject.keywordMolecular epidemiologyspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPrevalencespa
dc.subject.keywordBlvspa
dc.subject.keywordEnzootic bovine leukosisspa
dc.subject.keywordPrevalencespa
dc.subject.keywordViral genotypesspa
dc.titlePrevalence and molecular epidemiology of bovine leukemia virus in Colombian cattlespa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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