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Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia

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dc.creatorRamírez, Juan David
dc.creatorFlórez, Carolina
dc.creatorOlivera, Mario
dc.creatorBernal, María Consuelo
dc.creatorGiraldo, Julio Cesar
dc.creator.googleRamírez, Juan Davidspa
dc.creator.googleFlórez, Carolinaspa
dc.creator.googleOlivera, Mariospa
dc.creator.googleBernal, María Consuelospa
dc.creator.googleGiraldo, Julio Cesarspa
dc.date.accessioned2018-12-10T22:43:11Z
dc.date.available2018-12-10T22:43:11Z
dc.date.created2017-02-21
dc.date.issued2017
dc.description.abstractBlastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs/18S alleles in symptomatic (abdominal pain, anal pruritus, diarrhea, headache, nauseas and/or vomit) and asymptomatic children from nine geographical regions of Colombia. A total of 2026 fecal samples were collected as part of a national survey to estimate the frequency of intestinal parasites in children. A set of 256 samples that were Blastocystis positive was finally selected. The samples were submitted to DNA extraction, Real Time PCR and sequencing using Blastocystis-specific primers targeting the small subunit rRNA gene for ST identification. DNA of Ascaris lumbricoides (16.4%), Trichuris trichiura (8.2%), hookworms (Necator americanus/Ancylostoma duodenale) (7.3%), Giardia duodenalis (23.1%), Entamoeba complex (82%), Entamoeba coli (55%), Hymenolepis nana (0.8%), Endolimax nana (33.2%) and Neobalantidium coli (2.7%) was detected in the Blastocystispositive samples. We detected ST1 (21.4%), ST2 (19.5%), ST3 (55.5%), ST4 (0.8%), ST6 (2%) and ST7 (0.8%); alleles 1, 2, 4, 81, 82 and 83 for ST1; alleles 9, 11, 12, 15, 67, 71 and 73 for ST2; alleles 34, 36, 38, 45, 49, 55, 134 and 128 for ST3; allele 42 for ST4; allele 122 for ST6, and allele 142 for ST7. Further studies implementing high-resolution molecular markers are necessary to understand the dynamics of Blastocystis transmission and the role of this Stramenopila in health and disease. © 2017 Ramírez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0172586
dc.identifier.issnISSN 1932-6203
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18795
dc.language.isoengspa
dc.relation.citationIssueNo. 2
dc.relation.citationTitlePLoS ONE
dc.relation.citationVolumeVol. 12
dc.relation.ispartofPLoS ONE, ISSN: 1932-6203, Vol. 12/No. 2 (2017)spa
dc.relation.urihttps://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0172586&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/spa
dc.source.bibliographicCitationYoshikawa, H., Morimoto, K., Wu, Z., Singh, M., Hashimoto, T., Problems in speciation in the genus Blastocystis (2004) Trends Parasitol, 20, pp. 251-255. , PMID: 15147669spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectDolor abdominalspa
dc.subjectProtozoariospa
dc.subjectRibosomalspa
dc.subjectTaxonómicospa
dc.subjectAdolescentespa
dc.subjectViejo que picaspa
dc.subjectLombriz intestinalspa
dc.subjectInfección asintomáticaspa
dc.subjectBlastocistosisspa
dc.subjectNiñospa
dc.subjectEstudio controladospa
dc.subjectDiarreaspa
dc.subjectCódigo de barras de ADNspa
dc.subjectMadre endolímaxspa
dc.subjectEntamoebaspa
dc.subjectEntamoeba Colispa
dc.subjectSecuencia de genesspa
dc.subjectDistribución geográficaspa
dc.subjectGiardia intestinalisspa
dc.subjectDolor de cabezaspa
dc.subjectHumanospa
dc.subjectHimenolepis nanaspa
dc.subjectParásito del intestinospa
dc.subjectMasculinospa
dc.subjectDiagnóstico Molecularspa
dc.subjectTipificación molecularspa
dc.subjectNauseaspa
dc.subjectAsesino americanospa
dc.subjectNeobalantidium Colispa
dc.subjectNo humanospa
dc.subjectSecuencia de nucleótidosspa
dc.subjectReacción en cadena de la polimerasa en tiempo realspa
dc.subjectVómitosspa
dc.subjectEnfermedad asintomáticaspa
dc.subjectInfecciones por blastocistisspa
dc.subjectClasificaciónspa
dc.subjectEstudio comparativospa
dc.subjectCódigo de barras de ADNspa
dc.subjectHecesspa
dc.subjectVariación genéticaspa
dc.subjectGenéticaspa
dc.subjectEncuesta de Saludspa
dc.subjectInfección intestinalspa
dc.subjectAislamiento y Purificaciónspa
dc.subjectGeografía Médicaspa
dc.subjectInfección mixtaspa
dc.subjectParasitologíaspa
dc.subjectNiño preescolarspa
dc.subjectRibotipadospa
dc.subjectDiferencia de especiesspa
dc.subjectADN protozoariospa
dc.subjectARN protozoariospa
dc.subjectADN ribosomalspa
dc.subjectRna 18Sspa
dc.subjectAdolescentespa
dc.subjectAlelosspa
dc.subjectEnfermedades Asintomáticasspa
dc.subjectInfecciones por blastocistisspa
dc.subjectCoinfecciónspa
dc.subjectADNspa
dc.subjectReacción en cadena de la polimerasa en tiempo realspa
dc.subjectRibotipadospa
dc.subjectARN, Ribosomal, 18Sspa
dc.subjectEspecificidad de especiespa
dc.subject.keywordMedicaleng
dc.subject.keywordParasiticeng
dc.subject.keywordPreschooleng
dc.subject.keywordProtozoaneng
dc.subject.keywordSpecies Specificityeng
dc.subject.keywordBlastocystiseng
dc.subject.keywordFemaleeng
dc.subject.keywordRna, Ribosomal, 18Seng
dc.subject.keywordRibotypingeng
dc.subject.keywordChildeng
dc.subject.keywordReactionspa
dc.subject.keywordGeographyeng
dc.subject.keywordHumansspa
dc.subject.keywordIntestinal Diseaseseng
dc.subject.keywordRNAeng
dc.subject.keywordReal-Time Polymerase Chaineng
dc.subject.keywordDNAeng
dc.subject.keywordCoinfectioneng
dc.subject.keywordBlastocystis Infectionseng
dc.subject.keywordAlleleseng
dc.subject.keywordAsymptomatic Diseaseseng
dc.subject.keywordAdolescenteng
dc.subject.keywordRibosome Dnaeng
dc.subject.keywordProtozoal Dnaeng
dc.subject.keywordProtozoal Rnaeng
dc.subject.keywordSpecies Differenceeng
dc.subject.keywordRibotypingeng
dc.subject.keywordPreschool Childeng
dc.subject.keywordParasitologeng
dc.subject.keywordMixed Infectioneng
dc.subject.keywordMedical Geographyeng
dc.subject.keywordIsolation And Purificationeng
dc.subject.keywordIntestine Infectioneng
dc.subject.keywordHealth Surveyeng
dc.subject.keywordGeneticseng
dc.subject.keywordVariación genéticaeng
dc.subject.keywordGenetic Variationeng
dc.subject.keywordFeceseng
dc.subject.keywordDna Barcodingeng
dc.subject.keywordComparative Studyeng
dc.subject.keywordClassificationeng
dc.subject.keywordBlastocystis Infectionseng
dc.subject.keywordAsymptomatic Diseaseeng
dc.subject.keywordVomitingeng
dc.subject.keywordReactioneng
dc.subject.keywordReal Time Polymerase Chaineng
dc.subject.keywordNucleotide Sequenceeng
dc.subject.keywordNecator Americanuseng
dc.subject.keywordNonhumaneng
dc.subject.keywordMolecular Typingeng
dc.subject.keywordMolecular Diagnosiseng
dc.subject.keywordIntestine Parasiteeng
dc.subject.keywordMaleeng
dc.subject.keywordHymenolepis Nanaeng
dc.subject.keywordHumaneng
dc.subject.keywordHeadacheeng
dc.subject.keywordGeographic Distributioneng
dc.subject.keywordGene Sequenceeng
dc.subject.keywordEndolimax Nanaeng
dc.subject.keywordDna Barcodingeng
dc.subject.keywordControlled Studyeng
dc.subject.keywordBlastocystosiseng
dc.subject.keywordAsymptomatic Infectioneng
dc.subject.keywordAsymptomatic Infectioneng
dc.subject.keywordAnus Prurituseng
dc.subject.keywordAncylostoma Duodenaleeng
dc.subject.keywordTaxonomiceng
dc.subject.keywordAdolescenteng
dc.subject.keywordProtozoaneng
dc.subject.keywordAbdominal Paineng
dc.subject.keywordDiarrheaeng
dc.subject.keywordAllelero
dc.subject.lembInfecciones por Blastocystisspa
dc.subject.lembBlastocystisspa
dc.subject.lembGusanos parásitos e intestinalesspa
dc.titleBlastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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