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Plasmodium vivax in vitro continuous culture : The spoke in the wheel

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dc.creatorBermúdez, Maritza
dc.creatorMoreno‑Pérez, Darwin Andrés
dc.creatorArévalo-Pinzón, Gabriela
dc.creatorCurtidor, Hernando
dc.creatorPatarroyo, Manuel A.
dc.creator.googleBermúdez, Maritzaspa
dc.creator.googleMoreno‑Pérez, Darwin Andrésspa
dc.creator.googleArévalo-Pinzón, Gabrielaspa
dc.creator.googleCurtidor, Hernandospa
dc.creator.googlePatarroyo, Manuel Alfonsospa
dc.date.accessioned2019-03-06T15:02:11Z
dc.date.available2019-03-06T15:02:11Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractUnderstanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein's function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite's maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species' continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described. © 2018 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1186/s12936-018-2456-5
dc.identifier.issn1475-2875
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/19199
dc.language.isoengspa
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 17
dc.relation.ispartofMalaria Journal, ISSN:1475-2875, Vol. 17 (2018)spa
dc.relation.urihttps://malariajournal.biomedcentral.com/track/pdf/10.1186/s12936-018-2456-5spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitationBrackett, R.G., Cole, G.C., Green, T.J., Jacobs, R.L., In vitro propagation of Plasmodium falciparum for merozoite antigens (1979) Bull World Health Organ, 57, pp. 33-36. , 397007 2395713spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectCd71 Antigenspa
dc.subjectCell Maturationspa
dc.subjectContinuous Culturespa
dc.subjectControlled Studyspa
dc.subjectGenetic Strainspa
dc.subjectHost Cellspa
dc.subjectNonhumanspa
dc.subjectParasite Developmentspa
dc.subjectParasite Growthspa
dc.subjectParasite Isolationspa
dc.subjectParasite Phenomena And Functionsspa
dc.subjectParasite Replicationspa
dc.subjectParasite Strainspa
dc.subjectPlasmodium Vivaxspa
dc.subjectProtein Functionspa
dc.subjectReticulocytespa
dc.subjectReviewspa
dc.subjectTarget Cellspa
dc.subjectAnimalspa
dc.subjectChemistryspa
dc.subjectCulture Mediumspa
dc.subjectMicrobiological Examinationspa
dc.subjectParasitologyspa
dc.subjectPlasmodium Vivaxspa
dc.subjectProceduresspa
dc.subjectAnimalsspa
dc.subjectCulture Mediaspa
dc.subjectMicrobiological Techniquesspa
dc.subjectParasitologyspa
dc.subjectPlasmodium Vivaxspa
dc.subjectReticulocytesspa
dc.subject.ddcEnfermedadesspa
dc.subject.keywordDevelopment And Agingeng
dc.subject.keywordGrowtheng
dc.subject.lembMalariaspa
dc.subject.lembPlasmodium vivaxspa
dc.titlePlasmodium vivax in vitro continuous culture : The spoke in the wheelspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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