Ítem
Acceso Abierto

DNA barcoding for identification and phylogenetic inference of (Diptera; Ceratopogonidae) pollinators of cacao

Mostrar el registro sencillo de la publicación
dc.contributor.advisorRichardson, James Edward
dc.contributor.advisorSánchez Andrade, Adriana
dc.contributor.advisorSalazar Clavijo, Camilo Andrés
dc.contributor.advisorYockteng, Roxana
dc.creatorTamayo Ceballos, Iván Mateo
dc.creator.degreeBiólogo
dc.creator.degreeLevelPregrado
dc.date.accessioned2024-02-27T17:24:23Z
dc.date.available2024-02-27T17:24:23Z
dc.date.created2023
dc.date.embargoEndinfo:eu-repo/date/embargoEnd/2025-03-24
dc.descriptionLa pérdida de biodiversidad está ocurriendo a gran escala y la necesidad de monitorearla es cada vez más necesaria. El uso de técnicas morfológicas se puede mejorar con el uso de herramientas moleculares para ayudar a resolver los vacíos en el conocimiento de la diversidad y la historia evolutiva de las especies. El objetivo de esta investigación fue evaluar el uso del gen mitocondrial citocromo c oxidasa 1 (COI) como marcador de código de barras de ADN para caracterizar mosquitos como posibles polinizadores en cultivos de cacao colombianos. El estudio se implementó en los departamentos del Meta y Norte de Santander. Los taxones muestreados directamente de las flores se analizaron para evaluar su diversidad y relación filogenética utilizando la máxima verosimilitud (ML) y la inferencia bayesiana (BI). Generamos secuencias de aproximadamente 656 pb para 25 individuos Culicomorpha, 13 del Meta y 12 del Norte de Santander y descargamos 388 secuencias de las familias Ceratopogonidae y Chironomidae de GenBank. El análisis de las secuencias COI revela que nuestras secuencias se ubicaron en tres grupos de linajes de Ceratopogonidae (Forcipomyia, Dasyhelea y Stilobezzia) y cinco linajes no resueltos de Chironomidae. También encontramos que las especies que visitaron las flores de cacao en las plantaciones de Meta y Norte de Santander representaban dos grupos separados, que pueden estar influenciados por los procesos orogénicos de las montañas de los Andes. La reconstrucción filogenética indicó que la mayoría (n=17) de nuestras secuencias se resolvieron en el grupo Forcipomyia. Adicionalmente, ninguna de nuestras secuencias fue idéntica a las secuencias de GenBank, lo que refleja un sesgo de investigación para las regiones templadas del norte y la necesidad de más estudios sobre especies tropicales. Nuestros datos ofrecen nuevas secuencias moleculares de Ceratopogonidae colombianos para el desarrollo de un inventario global de especies polinizadoras de plantas de interés económico, como el cacao. Se sugiere también la necesidad de seguir muestreando taxones tropicales para esclarecer la historia evolutiva de estas familias de moscas.
dc.description.abstractBiodiversity loss is occurring on a large scale and the need to monitor it is becoming more and more necessary. The use of morphological techniques can be enhanced with the use of molecular tools to help resolve diversity and evolutionary history knowledge gaps. The objective of this research was to evaluate the use of the mitochondrial cytochrome c oxidase 1 (COI) gene as a DNA barcode marker to characterize possible pollinators of Colombian cacao crops. The study was implemented in the departments of Meta and Northern Santander, Colombia. Taxa sampled directly from flowers were analyzed to assess their diversity and phylogenetic relationships using Maximum-Likelihood (ML) and Bayesian Inference (BI). We generated sequences of approximately 656 bp for 25 Culicomorpha individuals, 13 from Meta and 12 from Northern Santander and downloaded 388 sequences of the Ceratopogonidae and Chironomidae families from GenBank. Analysis of the COI sequences reveals that our sequences were placed in three Ceratopogonidae lineages (Forcipomyia, Dasyhelea and Stilobezzia) and five unresolved lineages of Chironomidae. We also found that species that visited cacao flowers in Meta and Northern Santander plantations represented two separate guilds, which could have been influenced by the orogenic processes of the Andes Mountains. Phylogenetic reconstruction indicated that most (n=17) of our sequences were resolved in the Forcipomyia group. Additionally, none of our sequences were identical to any GenBank sequences, reflecting an investigative bias towards northern temperate regions and the need for more molecular studies on tropical species. Our data offer new DNA sequences of Colombian Ceratopogonidae for the development of a global inventory of pollinating species of plants of economic interest, such as cacao. The need to continue sampling tropical taxa is also suggested in order to clarify the evolutionary history of these families of flies.
dc.format.extent22 pp
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.48713/10336_38266
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/42298
dc.language.isospa
dc.publisherUniversidad del Rosario
dc.publisher.departmentFacultad de Ciencias Naturales
dc.publisher.programBiología
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.accesRightsinfo:eu-repo/semantics/embargoedAccess
dc.rights.accesoRestringido (Temporalmente bloqueado)
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.source.bibliographicCitationAdachi, J., & Hasegawa, M. (1996). Model of amino acid substitution in proteins encoded by mitochondrial DNA. Journal of Molecular Evolution, 42(4), 459-468. https://doi.org/10.1007/BF02498640
dc.source.bibliographicCitationAnisimova, M., Gil, M., Dufayard, J.-F., Dessimoz, C. & Gascuel, O. (2011). Survey of branch support methods demonstrates accuracy, power, and robustness of fast likelihoodbased approximation schemes. Systematic Biology, 60(5), 685-699. https://doi.org/10.1093/sysbio/syr041
dc.source.bibliographicCitationBartley, B. G. D. (Ed.). (2005). The genetic diversity of cacao and its utilization. CABI. https://doi.org/10.1079/9780851996196.0000
dc.source.bibliographicCitationBeckenbach, A. T., & Borkent, A. (2003). Molecular analysis of the biting midges (Diptera: Ceratopogonidae), based on mitochondrial cytochrome oxidase subunit 2. Molecular Phylogenetics and Evolution, 27(1), 21-35. https://doi.org/10.1016/s1055-7903(02)00395-0
dc.source.bibliographicCitationBorkent, A. (1991). The Ceratopogonidae (Diptera) of the Galápagos Islands, Ecuador with a discussion of their phylogenetic relationships and zoogeographic origins. Insect Systematics & Evolution, 22(1), 97-122. https://doi.org/10.1163/187631291X00336
dc.source.bibliographicCitationBorkent, A. (2001). Leptoconops (Diptera: Ceratopogonidae), the Earliest Extant Lineage of Biting Midge, Discovered in 120–122 Million-Year-Old Lebanese Amber. American Museum Novitates, 2001(3328), 1-12. https://doi.org/10.1206/0003- 0082(2001)328<0001:LDCTEE>2.0.CO;2
dc.source.bibliographicCitationBorkent, A. (2014). The pupae of the biting midges of the world (Diptera: Ceratopogonidae), with a generic key and analysis of the phylogenetic relationships between genera. Zootaxa, 3879, 1-327. https://doi.org/10.11646/zootaxa.3879.1.1
dc.source.bibliographicCitationBorkent, A. & G. R. Spinelli. (2007). Neotropical Ceratopogonidae (Diptera: Insecta). In: Adis, J., J. R. Arias, G. Rueda-Delgado & K. M. Wattzen (Eds): Aquatic Biodiversity in Latin America (ABLA). Vol 4. Pensoft Publishers, Sofia-Moscow, 198 pp. Revista de La Sociedad Entomológica Argentina, 67(1-2), Art. 1-2. https://www.biotaxa.org/RSEA/article/view/31038
dc.source.bibliographicCitationBorkent, A., & Dominiak, P. (2020). Catalog of the Biting Midges of the World (Diptera: Ceratopogonidae). Zootaxa, 4787(1), zootaxa.4787.1.1. https://doi.org/10.11646/zootaxa.4787.1.1
dc.source.bibliographicCitationBourguignon, T., Tang, Q., Ho, S. Y. W., Juna, F., Wang, Z., Arab, D. A., Cameron, S. L., Walker, J., Rentz, D., Evans, T. A., & Lo, N. (2018). Transoceanic Dispersal and Plate Tectonics Shaped Global Cockroach Distributions: Evidence from Mitochondrial Phylogenomics. Molecular Biology and Evolution, 35(4), 970-983. https://doi.org/10.1093/molbev/msy013
dc.source.bibliographicCitationBranco, S. M. de J., Silva, D. V. da, Lopes, U. V. & Corrêa, R. X. (2018). Characterization of the Sexual Self- and Cross-Compatibility in Genotypes of Cacao. American Journal of Plant Sciences, 9(9), Art. 9. https://doi.org/10.4236/ajps.2018.99131
dc.source.bibliographicCitationBridgemohan, P., Singh, K., Cazoe, E., Perry, G., Mohamed, A., & Bridgemohan, R. S. (2017). Cacao floral phenology and pollination: Implications for productivity in Caribbean Islands. Journal of Plant Breeding and Crop Science, 9(7), 106-117. https://doi.org/10.5897/JPBCS2016.0598
dc.source.bibliographicCitationCarpenter, S., Groschup, M. H., Garros, C., Felippe-Bauer, M. L., & Purse, B. V. (2013). Culicoides biting midges, arboviruses and public health in Europe. Antiviral Research, 100(1), 102-113. https://doi.org/10.1016/j.antiviral.2013.07.020
dc.source.bibliographicCitationChoufani, J., El-Halabi, W., Azar, D., & Nel, A. (2015). First fossil insect from Lower Cretaceous Lebanese amber in Syria (Diptera: Ceratopogonidae). Cretaceous Research, 54, 106-116. https://doi.org/10.1016/j.cretres.2014.12.006
dc.source.bibliographicCitationDamm, S., Schierwater, B. & Hadrys, H. (2010). An integrative approach to species discovery in odonates: From character-based DNA barcoding to ecology. Molecular Ecology, 19(18), 3881-3893. https://doi.org/10.1111/j.1365-294X.2010.04720.x
dc.source.bibliographicCitationDNA Barcoding Program. (2017, agosto 22). ForestGEO. https://forestgeo.si.edu/researchprograms/dna-barcoding-program
dc.source.bibliographicCitationDellinger, A. S., Pérez-Barrales, R., Michelangeli, F. A., Penneys, D. S., FernándezFernández, D. M., & Schönenberger, J. (2021). Low bee visitation rates explain pollinator shifts to vertebrates in tropical mountains. New Phytologist, 231(2), 864-877. https://doi.org/10.1111/nph.17390
dc.source.bibliographicCitationEagles, D., Deveson, T., Walker, P. J., Zalucki, M. P., & Durr, P. (2012). Evaluation of longdistance dispersal of Culicoides midges into northern Australia using a migration model. Medical and Veterinary Entomology, 26(3), 334-340. https://doi.org/10.1111/j.1365- 2915.2011.01005.x
dc.source.bibliographicCitationErickson, B. J., Young, A. M., Strand, M. A., & Erickson, E. H. (1987). Pollination biology of Theobroma and Herrania (Sterculiaceae). International Journal of Tropical Insect Science, 8(3), 301-310. https://doi.org/10.1017/S1742758400005282
dc.source.bibliographicCitationFolmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294-299.
dc.source.bibliographicCitationFreeman, J. A. (1945). Studies in the Distribution of Insects by Aerial Currents. Journal of Animal Ecology, 14(2), 128-154. https://doi.org/10.2307/1389
dc.source.bibliographicCitationFrimpong, E. A., Gordon, I., Kwapong, P. K., & Gemmill-Herren, B. (2009). Dynamics of cacao pollination: Tools and applications for surveying and monitoring cacao pollinators. International Journal of Tropical Insect Science, 29(2), 62-69. https://doi.org/10.1017/S1742758409990117
dc.source.bibliographicCitationFrimpong-Anin, K., Bosu, P., Adjaloo, M., Braimah, H. & Oduro, W. (2015). Some Facts About Cacao Pollination. Global Pollination Project-Ghana. ISBN: 9964-3-9352-0
dc.source.bibliographicCitationGostel, M. R., & Kress, W. J. (2022). The Expanding Role of DNA Barcodes: Indispensable Tools for Ecology, Evolution, and Conservation. Diversity, 14(3), 213. https://doi.org/10.3390/d14030213
dc.source.bibliographicCitationGuindon, S., Dufayard, J.-F., Lefort, V., Anisimova, M., Hordijk, W., & Gascuel, O. (2010). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59(3), 307-321. https://doi.org/10.1093/sysbio/syq010
dc.source.bibliographicCitationHebert, P. D. N., Cywinska, A., Ball, S. L., & deWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270(1512), 313-321. https://doi.org/10.1098/rspb.2002.2218
dc.source.bibliographicCitationHoang, D. T., Chernomor, O., von Haeseler, A., Minh, B. Q., & Vinh, L. S. (2018). UFBoot2: Improving the Ultrafast Bootstrap Approximation. Molecular Biology and Evolution, 35(2), 518-522. https://doi.org/10.1093/molbev/msx281
dc.source.bibliographicCitationHolzapfel, E., & Harrell, J. (1968). Transoceanic dispersal studies of insects. https://www.semanticscholar.org/paper/TRANSOCEANIC-DISPERSAL-STUDIES-OFINSECTS-Holzapfel-Harrell/5e5d6cffb83ae68da4ac989c56eb6684d6c001be
dc.source.bibliographicCitationHortal, J., de Bello, F., Diniz-Filho, J. A. F., Lewinsohn, T. M., Lobo, J. M. & Ladle, R. J. (2015). Seven shortfalls that beset large-scale knowledge of biodiversity. Annual Review of Ecology, Evolution, and Systematics, 46(1), 523-549. https://doi.org/10.1146/annurevecolsys-112414-054400
dc.source.bibliographicCitationHuelsenbeck, J. P., & Ronquist, F. (2001). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics (Oxford, England), 17(8), 754-755. https://doi.org/10.1093/bioinformatics/17.8.754
dc.source.bibliographicCitationKatoh, K., & Standley, D. M. (2013). MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Molecular Biology and Evolution, 30(4), 772-780. https://doi.org/10.1093/molbev/mst010
dc.source.bibliographicCitationKaufmann, T. (1975). Studies on the ecology and biology of a cacao pollinator, Forcipomyia squamipennis I. & M. (Diptera, Ceratopogonidae) in Ghana. Bulletin of Entomological Research, 65(2), 263-268. https://doi.org/10.1017/S0007485300005940
dc.source.bibliographicCitationKearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Meintjes, P., & Drummond, A. (2012). Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28(12), 1647-1649. https://doi.org/10.1093/bioinformatics/bts199
dc.source.bibliographicCitationKlein, A.-M., Vaissière, B. E., Cane, J. H., Steffan-Dewenter, I., Cunningham, S. A., Kremen, C., & Tscharntke, T. (2007). Importance of pollinators in changing landscapes for world crops. Proceedings of the Royal Society B: Biological Sciences, 274(1608), 303-313. https://doi.org/10.1098/rspb.2006.3721
dc.source.bibliographicCitationKobayashi, S., Denda, T., Placksanoi, J., Waengsothorn, S., Aryuthaka, C., Panha, S., & Izawa, M. (2019). The pollination system of the widely distributed mammal-pollinated Mucuna macrocarpa (Fabaceae) in the tropics. Ecology and Evolution, 9(11), 6276-6286. https://doi.org/10.1002/ece3.5201
dc.source.bibliographicCitationKress, W. J., García-Robledo, C., Uriarte, M., & Erickson, D. L. (2015). DNA barcodes for ecology, evolution, and conservation. Trends in Ecology & Evolution, 30(1), 25-35. https://doi.org/10.1016/j.tree.2014.10.008
dc.source.bibliographicCitationKrosch, M. N., Baker, A. M., Mather, P. B., & Cranston, P. S. (2011). Systematics and biogeography of the Gondwanan Orthocladiinae (Diptera: Chironomidae). Molecular Phylogenetics and Evolution, 59(2), 458-468. https://doi.org/10.1016/j.ympev.2011.03.003
dc.source.bibliographicCitationKumar, N. P., Rajavel, A. R., Natarajan, R., & Jambulingam, P. (2007). DNA Barcodes Can Distinguish Species of Indian Mosquitoes (Diptera: Culicidae). Journal of Medical Entomology, 44(1), 01-07. https://doi.org/10.1093/jmedent/41.5.01
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectCódigos de barras de ADN
dc.subjectCOI
dc.subjectCacao
dc.subjectCeratopogonidae
dc.subjectFilogenias
dc.titleDNA barcoding for identification and phylogenetic inference of (Diptera; Ceratopogonidae) pollinators of cacao
dc.typebachelorThesis
dc.type.documentTrabajo de grado
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaTrabajo de grado
local.department.reportFacultad de Ciencias Naturales
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
DNA_barcoding_for_identification_and_phylogenetic_inference_of_Diptera_Ceratopogonidae_Pollinators_of_cacao.pdf
Tamaño:
1.42 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Pregrado en Biología