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Identification of Six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay

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dc.creatorGalindo Hernández, Carolinaspa
dc.creatorAlvarez, Catalinaspa
dc.creatorGonzález, Camilaspa
dc.creatorAyala, Martha Stellaspa
dc.creatorLeón, Cielo Maritzaspa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-25T23:57:50Z
dc.date.available2020-05-25T23:57:50Z
dc.date.created2014spa
dc.description.abstractBackground: Leishmaniases are tropical zoonotic diseases, caused by parasites from the genus Leishmania. New World (NW) species are related to sylvatic cycles although urbanization processes have been reported in some South American Countries such as Colombia. This eco-epidemiological complexity imposes a challenge to the detection of circulating parasite species, not only related to human cases but also infecting vectors and reservoirs. Currently, no harmonized methods have been deployed to discriminate the NW Leishmania species. Findings: Herein, we conducted a systematic and mechanistic High-Resolution Melting (HRM) assay targeted to HSP70 and ITS1. Specific primers were designed that coupled with a HRM analyses permitted to discriminate six NW Leishmania species. In order to validate the herein described algorithm, we included 35 natural isolates obtained from human cases, insect vectors and mammals. Our genotyping assay allowed the correct assignment of the six NW Leishmania species (L. mexicana, L. infantum (chagasi), L. amazonensis, L. panamensis, L. guyanensis and L. braziliensis) based on reference strains. When the algorithm was applied to a set of well-characterized strains by means of PCR-RFLP, MLEE and monoclonal antibodies (MA) we observed a tailored concordance between the HRM and PCR-RFLP/MLEE/MA (KI = 1.0). Additionally, we tested the limit of detection for the HRM method showing that this is able to detect at least 10 equivalent-parasites per mL. Conclusions: This is a rapid and reliable method to conduct molecular epidemiology and host-parasite association studies in endemic areas. © 2014 Baleela et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s13071-014-0501-y
dc.identifier.issn17563305
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22754
dc.language.isoengspa
dc.publisherBioMed Central Ltd.spa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleParasites and Vectors
dc.relation.citationVolumeVol. 7
dc.relation.ispartofParasites and Vectors, ISSN:17563305, Vol.7, No.1 (2014)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84964696967&doi=10.1186%2fs13071-014-0501-y&partnerID=40&md5=f6dfb33f139c4103d421d9d2916ecef4spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordHeat shock protein 70spa
dc.subject.keywordprotozoaneng
dc.subject.keywordInternal transcribed spacer 1spa
dc.subject.keywordMonoclonal antibodyspa
dc.subject.keywordProtozoal dnaspa
dc.subject.keywordAccuracyspa
dc.subject.keywordAlgorithmspa
dc.subject.keywordArticlespa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDisease carrierspa
dc.subject.keywordGenotypespa
dc.subject.keywordHigh resolution melting analysisspa
dc.subject.keywordHumanspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordLeishmania amazonensisspa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordLeishmania guyanensisspa
dc.subject.keywordLeishmania infantumspa
dc.subject.keywordLeishmania mexicanaspa
dc.subject.keywordLeishmania panamensisspa
dc.subject.keywordLimit of detectionspa
dc.subject.keywordMultilocus enzyme electrophoresisspa
dc.subject.keywordNonhumanspa
dc.subject.keywordParasite identificationspa
dc.subject.keywordParasite isolationspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordReliabilityspa
dc.subject.keywordRestriction fragment length polymorphismspa
dc.subject.keywordAnimalspa
dc.subject.keywordChemistryspa
dc.subject.keywordClassificationspa
dc.subject.keywordEvaluation studyspa
dc.subject.keywordGenetic proceduresspa
dc.subject.keywordGeneticsspa
dc.subject.keywordGenotypespa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordLeishmaniasisspa
dc.subject.keywordMammalspa
dc.subject.keywordParasitologyspa
dc.subject.keywordTransition temperaturespa
dc.subject.keywordAnimalsspa
dc.subject.keywordDnaeng
dc.subject.keywordGenetic techniquesspa
dc.subject.keywordGenotypespa
dc.subject.keywordHumansspa
dc.subject.keywordInsect vectorsspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordLeishmaniasisspa
dc.subject.keywordMammalsspa
dc.subject.keywordTransition temperaturespa
dc.subject.keywordGenotypingspa
dc.subject.keywordHigh-resolution meltingspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordReal-time pcrspa
dc.titleIdentification of Six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assayspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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