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microRNA Expression Profiling in Young Prostate Cancer Patients

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dc.creatorValera, Vladimir Aspa
dc.creatorParra-Medina, Rafaelspa
dc.creatorWalter, Beatriz Aspa
dc.creatorPinto, Peterspa
dc.creatorMerino, Maria Jspa
dc.date.accessioned2020-06-11T13:21:45Z
dc.date.available2020-06-11T13:21:45Z
dc.date.created2020spa
dc.description.abstractMicroRNAs (miRNAs) are small, non-coding RNA molecules with multiple roles in many biological processes. Few studies have shown the molecular characteristics in younger prostate cancer (PCa) patients. In this study, we performed miRNA profiling in young PCa (EO-PCa) cases compared with PCa arising in older men (LO-PCa).Experimental Design: Formalin-fixed, paraffin embedded tissue was used. miRNA was extracted for PCR array and NanoString methods. Relative miRNAs expression levels were obtained by comparing young vs older men, and young PCa tumor samples vs normal epithelium.Results: miRNA profiling showed a different expression pattern in PCa arising in younger men, and young PCa tumoral and its normal counterpart. Nine miRNAs (hsa-miR-140-5p, hsa-miR-146a, hsa-miR-29b, hsa-miR-9, hsa-miR-124-3p, hsa-let-7f-5p, hsa-miR-184, hsa-miR-373, hsa-miR-146b-5p) showed differences in the expression compared to LO-PCa. Fourteen miRNAs were significantly up-regulated (miR-1973, miR-663a, miR-575, miR-93-5p, miR-630, miR-600, miR-494, miR-150-5p, miR-137, miR-25-3p, miR-375, miR-489, miR-888-5p, miR-142-3p), while 9 were found down-regulated (miR-21-5p, miR-363-3p, miR-205-5p, miR-548ai, miR-3195, 145-5p, miR-143-3p, miR-222-3p, miR-221-3p) comparing young PCa tumoral tissue compared to normal counterpart. The higher expression of miR-600 and miR-137 were associated with high Gleason score, extraprostatic extension and lymphatic invasion.Conclusion: These results suggest that PCa in younger patients has a different expression profile compared to normal tissue and PCa arising in older man. Differentially expressed miRNAs provide insights of molecular mechanisms involve in this PCa subtype.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.7150/jca.37842
dc.identifier.issn1837-9664
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/24891
dc.language.isoeng
dc.publisherJournal of Cancerspa
dc.relation.citationEndPage4114
dc.relation.citationIssueNo. 14
dc.relation.citationStartPage4106
dc.relation.citationTitleJournal of Cancer
dc.relation.citationVolumeVol. 11
dc.relation.ispartofJournal of Cancer, ISSN: 1837-9664, Vol.11, No.14 (2020); pp. 4106-4114spa
dc.relation.urihttps://www.jcancer.org/v11p4106.pdfspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordMicroRNAsspa
dc.subject.keywordprostatespa
dc.subject.keywordearly onsetspa
dc.subject.keywordyoungspa
dc.titlemicroRNA Expression Profiling in Young Prostate Cancer Patientsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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