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Plasmodium vivax lineages: geographical distribution, tandem repeat polymorphism, and phylogenetic relationship

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dc.audienceComunidad Rosaristaspa
dc.creatorPrajapati, Surendra K
dc.creatorJoshi, Hema
dc.creatorShalini, Sneh
dc.creatorPatarroyo, Manuel A.
dc.creatorSuwanarusk, Rossarin
dc.creatorKumar, Ashwani
dc.creatorSharma, Surya K
dc.creatorEapen, Alex
dc.creatorDev, Vas
dc.creatorBhatt, Rajendra
dc.creatorValecha, Neena
dc.creatorNosten, Francois
dc.creatorRizvi, Moshahid A
dc.creatorDash, Aditya
dc.creator.googlePrajapati, Surendra K
dc.creator.googleJoshi, Hema
dc.creator.googleShalini, Sneh
dc.creator.googlePatarroyo, Manuel A.
dc.creator.googleSuwanarusk, Rossarin
dc.creator.googleKumar, Ashwani
dc.creator.googleSharma, Surya K
dc.creator.googleEapen, Alex
dc.creator.googleDev, Vas
dc.creator.googleBhatt, Rajendra M
dc.creator.googleValecha, Neena
dc.creator.googleNosten, Francois
dc.creator.googleRizvi, Moshahid A
dc.creator.googleDash, Aditya P
dc.date.accessioned2014-08-12T16:34:21Z
dc.date.available2014-08-12T16:34:21Z
dc.date.created2011-12-19
dc.date.issued2011
dc.description.abstractBackground: Multi-drug resistance and severe/ complicated cases are the emerging phenotypes of vivax malaria, which may deteriorate current anti-malarial control measures. The emergence of these phenotypes could be associated with either of the two Plasmodium vivax lineages. The two lineages had been categorized as Old World and New World, based on geographical sub-division and genetic and phenotypical markers. This study revisited the lineage hypothesis of P. vivax by typing the distribution of lineages among global isolates and evaluated their genetic relatedness using a panel of new mini-satellite markers. Methods: 18S SSU rRNA S-type gene was amplified from 420 Plasmodium vivax field isolates collected from different geographical regions of India, Thailand and Colombia as well as four strains each of P. vivax originating from Nicaragua, Panama, Thailand (Pak Chang), and Vietnam (ONG). A mini-satellite marker panel was then developed to understand the population genetic parameters and tested on a sample subset of both lineages. Results: 18S SSU rRNA S-type gene typing revealed the distribution of both lineages (Old World and New World) in all geographical regions. However, distribution of Plasmodium vivax lineages was highly variable in every geographical region. The lack of geographical sub-division between lineages suggests that both lineages are globally distributed. Ten mini-satellites were scanned from the P. vivax genome sequence; these tandem repeats were located in eight of the chromosomes. Mini-satellites revealed substantial allelic diversity (7-21, AE = 14.6 +/- 2.0) and heterozygosity (He = 0.697-0.924, AE = 0.857 +/- 0.033) per locus. Mini-satellite comparison between the two lineages revealed high but similar pattern of genetic diversity, allele frequency, and high degree of allele sharing. A Neighbour-Joining phylogenetic tree derived from genetic distance data obtained from ten mini-satellites also placed both lineages together in every cluster. Conclusions: The global lineage distribution, lack of genetic distance, similar pattern of genetic diversity, and allele sharing strongly suggested that both lineages are a single species and thus new emerging phenotypes associated with vivax malaria could not be clearly classified as belonging to a particular lineage on basis of their geographical origin.eng
dc.format.mediumRecurso electrónicospa
dc.format.mimetypeapplication/pdf
dc.format.tipoDocumentospa
dc.identifier.issnISSN:1475-2875
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/8809
dc.language.isoeng
dc.publisherUniversidad del Rosariospa
dc.relation.citationIssueNo. 374
dc.relation.citationTitleMALARIA JOURNAL
dc.relation.citationVolumeVol. 10
dc.relation.ispartofMALARIA JOURNAL ISSN: 1475-2875 V. 10 N. 374 Dic 19, 2011spa
dc.relation.urihttp://apps.webofknowledge.com.ez.urosario.edu.co/full_record.do?product=UA&search_mode=GeneralSearch&qid=3&SID=2FeAM82FH4kCimTQW7c&page=1&doc=1
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto completo)spa
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.ddcEnfermedades
dc.subject.decsFarmacologíaspa
dc.subject.decsInmunologíaspa
dc.subject.decsMalariaspa
dc.subject.decsPlasmodiumspa
dc.subject.keywordMEROZOITE SURFACE PROTEIN-3-ALPHAeng
dc.subject.keywordMICROSATELLITE MARKERSeng
dc.subject.keywordPOPULATION-STRUCTUREeng
dc.subject.keywordGENETIC-STRUCTUREeng
dc.subject.keywordMALARIAeng
dc.subject.keywordDIVERSITYeng
dc.subject.keywordINDIAeng
dc.subject.keywordFALCIPARUMeng
dc.subject.keywordSEQUENCESeng
dc.subject.keywordSOFTWAREeng
dc.titlePlasmodium vivax lineages: geographical distribution, tandem repeat polymorphism, and phylogenetic relationshipspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaArtículospa
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