Ítem
Solo Metadatos

Major changes in chromosomal somy, gene expression and gene dosage driven by SbIII in Leishmania braziliensis and Leishmania panamensis

dc.creatorPatino, Luz H.spa
dc.creatorImamura, Hideospa
dc.creatorCruz-Saavedra, Lissaspa
dc.creatorPavia, Paulaspa
dc.creatorMuskus, Carlosspa
dc.creatorMéndez-Sarmiento, Claudia
dc.creatorDujardin, Jean Claudespa
dc.creatorRamírez, Juan David
dc.date.accessioned2020-05-26T00:00:56Z
dc.date.available2020-05-26T00:00:56Z
dc.date.created2019spa
dc.description.abstractLeishmania braziliensis and Leishmania panamensis are two species clinically and epidemiologically important, among others because of their relative resistance to first-line drugs (antimonials). The precise mechanism underlying the ability of these species to survive antimony treatment remains unknown. Therefore, elucidating the pathways mediating drug resistance is essential. We herein experimentally selected resistance to trivalent antimony (SbIII) in the reference strains of L. braziliensis (MHOM/BR75/M2904) and L. panamensis (MHOM/COL/81L13) and compared whole genome and transcriptome alterations in the culture promastigote stage. The results allowed us to identify differences in somy, copy number variations in some genes related to antimony resistance and large-scale copy number variations (deletions and duplications) in chromosomes with no somy changes. We found mainly in L. braziliensis, a direct relation between the chromosomal/local copy number variation and the gene expression. We identified differentially expressed genes in the resistant lines that are involved in antimony resistance, virulence, and vital biological processes in parasites. The results of this study may be useful for characterizing the genetic mechanisms of these Leishmania species under antimonial pressure, and for clarifying why the parasites are resistant to first-line drug treatments. © 2019, The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1038/s41598-019-45538-9
dc.identifier.issn20452322
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23290
dc.language.isoengspa
dc.publisherNature Publishing Groupspa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleScientific Reports
dc.relation.citationVolumeVol. 9
dc.relation.ispartofScientific Reports, ISSN:20452322, Vol.9, No.1 (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85068227989&doi=10.1038%2fs41598-019-45538-9&partnerID=40&md5=767e569b86e731e4d6f53af8cc435d9fspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordChanges-chromosomalspa
dc.subject.keywordgenespa
dc.subject.keywordexpressionspa
dc.subject.keywordgenespa
dc.subject.keyworddosagespa
dc.subject.keyworddrivenspa
dc.subject.keywordbyspa
dc.subject.keywordSbIIIspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordbraziliensisspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordpanamensisspa
dc.titleMajor changes in chromosomal somy, gene expression and gene dosage driven by SbIII in Leishmania braziliensis and Leishmania panamensisspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Colecciones