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Sarconesin II, a New Antimicrobial Peptide Isolated from Sarconesiopsis magellanica Excretions and Secretions

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dc.creatorDíaz-Roa, Andreaspa
dc.creatorEspinoza-Culupú, Abrahamspa
dc.creatorTorres-García, Orlandospa
dc.creatorBorges, Monamaris M.spa
dc.creatorAvino, Ivan N.spa
dc.creatorAlves, Flávio L.spa
dc.creatorMiranda, Antoniospa
dc.creatorPatarroyo, Manuel A.spa
dc.creatorda Silva, Pedro I.spa
dc.creatorBello, Felio J.spa
dc.date.accessioned2020-05-25T23:57:13Z
dc.date.available2020-05-25T23:57:13Z
dc.date.created2019spa
dc.description.abstractAntibiotic resistance is at dangerous levels and increasing worldwide. The search for new antimicrobial drugs to counteract this problem is a priority for health institutions and organizations, both globally and in individual countries. Sarconesiopsis magellanica blowfly larval excretions and secretions (ES) are an important source for isolating antimicrobial peptides (AMPs). This study aims to identify and characterize a new S. magellanica AMP. RP-HPLC was used to fractionate ES, using C18 columns, and their antimicrobial activity was evaluated. The peptide sequence of the fraction collected at 43.7 min was determined by mass spectrometry (MS). Fluorescence and electronic microscopy were used to evaluate the mechanism of action. Toxicity was tested on HeLa cells and human erythrocytes; physicochemical properties were evaluated. The molecule in the ES was characterized as sarconesin II and it showed activity against Gram-negative (Escherichia coli MG1655, Pseudomonas aeruginosa ATCC 27853, P. aeruginosa PA14) and Gram-positive (Staphylococcus aureus ATCC 29213, Micrococcus luteus A270) bacteria. The lowest minimum inhibitory concentration obtained was 1.9 µM for M. luteus A270; the AMP had no toxicity in any cells tested here and its action in bacterial membrane and DNA was confirmed. Sarconesin II was documented as a conserved domain of the ATP synthase protein belonging to the Fli-1 superfamily. The data reported here indicated that peptides could be alternative therapeutic candidates for use in infections against Gram-negative and Gram-positive bacteria and eventually as a new resource of compounds for combating multidrug-resistant bacteria. © 2019 by the authors.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3390/molecules24112077
dc.identifier.issn14203049
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22630
dc.language.isoengspa
dc.publisherMDPI AGspa
dc.relation.citationIssueNo. 11
dc.relation.citationTitleMolecules
dc.relation.citationVolumeVol. 24
dc.relation.ispartofMolecules, ISSN:14203049, Vol.24, No.11 (2019)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85066733219&doi=10.3390%2fmolecules24112077&partnerID=40&md5=899017a0944f9e84d5e8cf2df811b987spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAntiinfective agentspa
dc.subject.keyworddrugeng
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalspa
dc.subject.keywordBacteriumspa
dc.subject.keywordBiosynthesisspa
dc.subject.keywordChemical phenomenaspa
dc.subject.keywordChemistryspa
dc.subject.keywordDipteraspa
dc.subject.keywordDose responsespa
dc.subject.keywordDrug effectspa
dc.subject.keywordHigh performance liquid chromatographyspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMass spectrometryspa
dc.subject.keywordMetabolismspa
dc.subject.keywordMicrobial sensitivity testspa
dc.subject.keywordMolecular modelspa
dc.subject.keywordProtein conformationspa
dc.subject.keywordStructure activity relationspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordAnti-bacterial agentsspa
dc.subject.keywordAntimicrobial cationic peptidesspa
dc.subject.keywordBacteriaspa
dc.subject.keywordChemical phenomenaspa
dc.subject.keywordChromatographyeng
dc.subject.keywordDipteraspa
dc.subject.keywordDose-response relationshipeng
dc.subject.keywordMass spectrometryspa
dc.subject.keywordMicrobial sensitivity testsspa
dc.subject.keywordModelseng
dc.subject.keywordProtein conformationspa
dc.subject.keywordStructure-activity relationshipspa
dc.subject.keywordAlpha-helixspa
dc.subject.keywordAntimicrobial peptidespa
dc.subject.keywordCalliphoridaespa
dc.subject.keywordDrugspa
dc.subject.keywordSarconesiopsis magellanicaspa
dc.titleSarconesin II, a New Antimicrobial Peptide Isolated from Sarconesiopsis magellanica Excretions and Secretionsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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