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Plasmodium falciparum rhoptry neck protein 5 peptides bind to human red blood cells and inhibit parasite invasion

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dc.creatorCurtidor, Hernando
dc.creatorPatiño, Liliana C.spa
dc.creatorArévalo-Pinzón, Gabrielaspa
dc.creatorVanegas, Magnoliaspa
dc.creatorPatarroyo, Manuel E.spa
dc.creatorPatarroyo, Manuel A.spa
dc.date.accessioned2020-05-25T23:58:03Z
dc.date.available2020-05-25T23:58:03Z
dc.date.created2014spa
dc.description.abstractPlasmodium falciparum malaria parasite invasion of erythrocytes is an essential step in host infection and the proteins involved in such invasion are the main target in developing an antimalarial vaccine. Secretory organelle-derived proteins (micronemal AMA1 protein and the RON2, 4, and 5 rhoptry neck proteins) have been recently described as components of moving junction complex formation allowing merozoites to move into a newly created parasitophorous vacuole. This study led to identifying RON5 regions involved in binding to human erythrocytes by using a highly robust, sensitive and specific receptor-ligand interaction assay; it is further shown that the RON5 protein remains highly conserved throughout different parasite strains. It is shown that the binding peptide-erythrocyte interaction is saturable and sensitive to chymotrypsin and trypsin. Invasion inhibition assays using erythrocyte binding peptides showed that the RON5-erythrocyte interaction could be critical for merozoite invasion of erythrocytes. This work provides evidence (for the first time) suggesting a fundamental role for RON5 in erythrocyte invasion. © 2013 Elsevier Inc.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.peptides.2013.07.028
dc.identifier.issn1969781
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22793
dc.language.isoengspa
dc.publisherElsevier Inc.spa
dc.relation.citationEndPage217
dc.relation.citationStartPage210
dc.relation.citationTitlePeptides
dc.relation.citationVolumeVol. 53
dc.relation.ispartofPeptides, ISSN:1969781, Vol.53,(2014); pp. 210-217spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84899437196&doi=10.1016%2fj.peptides.2013.07.028&partnerID=40&md5=9cea022e232e0b56ee56f32803f670cdspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordChymotrypsinspa
dc.subject.keywordimmunoelectroneng
dc.subject.keywordPlasmodium falciparum rhoptry neck protein 5 peptide derivativespa
dc.subject.keywordProteinspa
dc.subject.keywordRon5 proteinspa
dc.subject.keywordTrypsinspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordMalaria vaccinespa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordArticlespa
dc.subject.keywordAssayspa
dc.subject.keywordCell interactionspa
dc.subject.keywordCell invasionspa
dc.subject.keywordControlled studyspa
dc.subject.keywordErythrocytespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordMerozoitespa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordReceptor ligand interaction assayspa
dc.subject.keywordCell culturespa
dc.subject.keywordCircular dichroismspa
dc.subject.keywordErythrocytespa
dc.subject.keywordImmunoelectron microscopyspa
dc.subject.keywordImmunologyspa
dc.subject.keywordMetabolismspa
dc.subject.keywordParasitologyspa
dc.subject.keywordPathogenicityspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordCellseng
dc.subject.keywordCircular dichroismspa
dc.subject.keywordErythrocytesspa
dc.subject.keywordHumansspa
dc.subject.keywordMalaria vaccinesspa
dc.subject.keywordMerozoitesspa
dc.subject.keywordMicroscopyeng
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordBindingspa
dc.subject.keywordMalariaspa
dc.subject.keywordMerozoitespa
dc.subject.keywordRhoptry neckspa
dc.subject.keywordSynthetic peptidespa
dc.titlePlasmodium falciparum rhoptry neck protein 5 peptides bind to human red blood cells and inhibit parasite invasionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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