Ítem
Acceso Abierto

Identification and characterization of the Plasmodium vivax thrombospondin-related apical merozoite protein

Mostrar el registro sencillo de la publicación
dc.creatorMongui, Alvarospa
dc.creatorAngel, Diana Ispa
dc.creatorMoreno-Perez, Darwin Aspa
dc.creatorVillarreal-Gonzalez, Silvanaspa
dc.creatorAlmonacid, Hanniaspa
dc.creatorVanegas, Magnoliaspa
dc.creatorPatarroyo, Manuel A.
dc.date.accessioned2020-05-25T23:56:53Z
dc.date.available2020-05-25T23:56:53Z
dc.date.created2010spa
dc.description.abstractBackground. Malaria caused by Plasmodium vivax is a major public health problem worldwide that affects 70-80 million people in the Middle East, Asia, Western Pacific, South America and the Caribbean. Despite its epidemiological importance, few antigens from this parasite species have been characterized to date compared to Plasmodium falciparum, due in part to the difficulties of maintaining an in vitro culture of P. vivax. This study describes the identification of the P. falciparum thrombospondin-related apical merozoite protein homologue in P. vivax (PvTRAMP) and examines its potential to be further evaluated as vaccine candidate. Methods. The gene encoding PvTRAMP was identified through an extensive search of the databases hosting the genome sequence of P. vivax. Genes adjacent to pvtramp were identified in silico to determine the degree of similarity between the protein sequences encoded by equivalent chromosomic fragments in P. falciparum and Plasmodium knowlesi. The pvtramp gene was amplified from cDNA of P. vivax schizont stages, cloned and expressed in Escherichia coli. Anti-PvTRAMP antisera was obtained by inoculating rabbits with PvTRAMP B cell epitopes produced as synthetic peptides in order to assess its recognition in parasite lysates by Western blot and in intact parasites by indirect immunofluorescence. The recognition of recombinant PvTRAMP by sera from P. vivax-infected individuals living in endemic areas was also assessed by ELISA. Results. The PfTRAMP homologue in P. vivax, here denoted as PvTRAMP, is a 340-amino-acid long antigen encoded by a single exon that could have a potential role in cytoadherence, as indicated by the presence of a thrombospondin structural homology repeat (TSR) domain. According to its transcription and expression profile, PvTRAMP is initially located at the parasite's apical end and later on the parasite surface. Recombinant PvTRAMP is recognized by sera from infected patients, therefore, indicating that it is targeted by the immune system during a natural infection with P. vivax. Conclusions. The results of this work support conducting further studies with PvTRAMP to evaluate its immunogenicity and protection-inducing ability in the Aotus animal model. © 2010 Mongui et al; licensee BioMed Central Ltd.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/1475-2875-9-283
dc.identifier.issn14752875
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22551
dc.language.isoengspa
dc.relation.citationIssueNo. 1
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 9
dc.relation.ispartofMalaria Journal, ISSN:14752875, Vol.9, No.1 (2010)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-77957741156&doi=10.1186%2f1475-2875-9-283&partnerID=40&md5=8132bec08bdea247db7569ab857a589cspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordAntiserumspa
dc.subject.keywordfluorescenceeng
dc.subject.keywordEpitopespa
dc.subject.keywordRecombinant proteinspa
dc.subject.keywordSynthetic peptidespa
dc.subject.keywordThrombospondinspa
dc.subject.keywordThrombospondin related apical merozoite proteinspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordComplementary dnaspa
dc.subject.keywordMalaria vaccinespa
dc.subject.keywordMembrane antigenspa
dc.subject.keywordParasite antigenspa
dc.subject.keywordProtozoal dnaspa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordProtozoon antibodyspa
dc.subject.keywordThrombospondinspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimal cellspa
dc.subject.keywordAotusspa
dc.subject.keywordArticlespa
dc.subject.keywordCell lysatespa
dc.subject.keywordChromosomespa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordEscherichia colispa
dc.subject.keywordExonspa
dc.subject.keywordGenespa
dc.subject.keywordGene amplificationspa
dc.subject.keywordGene expression profilingspa
dc.subject.keywordGene identificationspa
dc.subject.keywordGene sequencespa
dc.subject.keywordGenetic transcriptionspa
dc.subject.keywordImmune systemspa
dc.subject.keywordImmunofluorescencespa
dc.subject.keywordImmunogenicityspa
dc.subject.keywordInoculationspa
dc.subject.keywordMolecular cloningspa
dc.subject.keywordNonhumanspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordPlasmodium knowlesispa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordProtein analysisspa
dc.subject.keywordProtein domainspa
dc.subject.keywordPvtramp genespa
dc.subject.keywordRabbitspa
dc.subject.keywordSchizontspa
dc.subject.keywordStructural homologyspa
dc.subject.keywordWestern blottingspa
dc.subject.keywordAnimalspa
dc.subject.keywordBiologyspa
dc.subject.keywordBloodspa
dc.subject.keywordColombiaspa
dc.subject.keywordFluorescence microscopyspa
dc.subject.keywordGene expressionspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHumanspa
dc.subject.keywordImmunologyspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMerozoitespa
dc.subject.keywordSequence homologyspa
dc.subject.keywordAnimalsspa
dc.subject.keywordAntibodieseng
dc.subject.keywordAntigenseng
dc.subject.keywordAntigenseng
dc.subject.keywordBlottingeng
dc.subject.keywordCloningeng
dc.subject.keywordColombiaspa
dc.subject.keywordComputational biologyspa
dc.subject.keywordDnaeng
dc.subject.keywordDnaeng
dc.subject.keywordEnzyme-linked immunosorbent assayspa
dc.subject.keywordEscherichia colispa
dc.subject.keywordGene expressionspa
dc.subject.keywordHumansspa
dc.subject.keywordMalaria vaccinesspa
dc.subject.keywordMerozoitesspa
dc.subject.keywordMicroscopyeng
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordRabbitsspa
dc.subject.keywordSequence homologyeng
dc.subject.keywordThrombospondinsspa
dc.titleIdentification and characterization of the Plasmodium vivax thrombospondin-related apical merozoite proteinspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
1475-2875-9-283.pdf
Tamaño:
2.26 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos