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Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

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dc.creatorRamírez, Juan David
dc.creatorHerrera, Giovannyspa
dc.creatorMuskus, Carlosspa
dc.creatorMéndez-Sarmiento, Claudia
dc.creatorDuque, María Claraspa
dc.creatorButcher, Robertspa
dc.date.accessioned2020-05-25T23:55:33Z
dc.date.available2020-05-25T23:55:33Z
dc.date.created2019spa
dc.description.abstractAim: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. Methods: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. Results: The limit of detection of the assay on the ddPCR platform was much higher than the published limit of detection of the same assay on the qPCR platform (100 vs 1 parasites/mL, respectively). Conclusion: While the performance of this assay in ddPCR format was acceptable for research purposes, it is not sufficient for clinical diagnostic purposes. The assay is more suited to the qPCR platform. © 2018 The Author(s)eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.ijid.2018.10.029
dc.identifier.issn12019712
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22126
dc.language.isoengspa
dc.publisherElsevier B.V.spa
dc.relation.citationEndPage3
dc.relation.citationStartPage1
dc.relation.citationTitleInternational Journal of Infectious Diseases
dc.relation.citationVolumeVol. 79
dc.relation.ispartofInternational Journal of Infectious Diseases, ISSN:12019712, Vol.79,(2019); pp. 1-3spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85057876716&doi=10.1016%2fj.ijid.2018.10.029&partnerID=40&md5=df03529c552cdaa5301dfc4023ee8107spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordProtozoal DNAspa
dc.subject.keywordProtozoal DNAspa
dc.subject.keywordArticlespa
dc.subject.keywordDroplet digital polymerase chain reactionspa
dc.subject.keywordHumanspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordLeishmania braziliensisspa
dc.subject.keywordLeishmania donovanispa
dc.subject.keywordLeishmania guyanensisspa
dc.subject.keywordLeishmania infantumspa
dc.subject.keywordLeishmania majorspa
dc.subject.keywordLeishmania mexicanaspa
dc.subject.keywordLeishmania panamensisspa
dc.subject.keywordLimit of detectionspa
dc.subject.keywordMicroorganism detectionspa
dc.subject.keywordMolecular pathologyspa
dc.subject.keywordSkin leishmaniasisspa
dc.subject.keywordTask performancespa
dc.subject.keywordValidation studyspa
dc.subject.keywordEvaluation studyspa
dc.subject.keywordFemalespa
dc.subject.keywordGeneticsspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordParasitologyspa
dc.subject.keywordProceduresspa
dc.subject.keywordReal time polymerase chain reactionspa
dc.subject.keywordSensitivity and specificityspa
dc.subject.keywordSkin leishmaniasisspa
dc.subject.keywordDNAeng
dc.subject.keywordFemalespa
dc.subject.keywordHumansspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordLeishmaniasiseng
dc.subject.keywordReal-Time Polymerase Chain Reactionspa
dc.subject.keywordSensitivity and Specificityspa
dc.subject.keywordCutaneous Leishmaniasisspa
dc.subject.keywordDdpcrspa
dc.subject.keywordLeishmaniaspa
dc.subject.keywordMolecular diagnosisspa
dc.titleDevelopment of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patientsspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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