Ítem
Solo Metadatos

Shortening and modifying the 1513 MSP-1 peptide’s ?-helical region induces protection against malaria

Mostrar el registro sencillo de la publicación
dc.creatorEspejo, Fabiolaspa
dc.creatorBermúdez, Adrianaspa
dc.creatorTorres, Elizabethspa
dc.creatorUrquiza, Mauriciospa
dc.creatorRodr??guez, Raúlspa
dc.creatorLópez, Yolandaspa
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-08-06T16:20:16Z
dc.date.available2020-08-06T16:20:16Z
dc.date.created2004-03-05spa
dc.description.abstractImmunogenic and protective peptide sequences are of prime importance in the search for an anti-malarial vaccine. The MSP-1 conserved and semi-conserved sequences have been shown to contain red blood cell (RBC) membrane high affinity binding peptides (HABP). HABP 1513 sequence (42GYSLFQKEKMVLNEGTSGTA61), from this protein’s N-terminal, has been shown to possess a T-epitope; however, it did not induce a humoral immune response or complete protection when evaluated in Aotus monkeys. Analogue peptides with critical binding residues replaced by amino acids with similar mass but different charge were synthesised and tested for immunogenicity and protectivity in monkey. NMR studies correlated structural behaviour with biological function. Non-immunogenic and non-protective 1513 native peptide presented a helical fragment between residues L4 and E14. C-terminal, 5-residue-shorter, non-immunogenic, non-protective peptide 17894 contained an ?-helix from Q6 to L12 residues. Immunogenic and protective peptide 13946 presented a shorter ?-helix between K7 to N13 residues. These data suggest that changing certain residues permits better peptide fit within the MHC class II–peptide–TCR complex, thus activating the immune system and inducing a protective immune response.spa
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2004.01.072
dc.identifier.issnISSN: 0006-291X
dc.identifier.issnEISSN: 1090-2104
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/25939
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage427
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage418
dc.relation.citationTitleBiochemical and Biophysical Research Communications
dc.relation.citationVolumeVol. 315
dc.relation.ispartofBiochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.315, No.2 (2004); pp.418-427spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0006291X04001147spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceBiochemical and Biophysical Research Communicationsspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordMalariaspa
dc.subject.keywordNMRPspa
dc.subject.keywordlasmodium falciparumspa
dc.subject.keywordStructurespa
dc.subject.keywordSynthetic vaccinespa
dc.subject.keywordMSP-1spa
dc.subject.keywordMHCspa
dc.titleShortening and modifying the 1513 MSP-1 peptide’s ?-helical region induces protection against malariaspa
dc.title.TranslatedTitleAcortar y modificar la región ?-helicoidal del péptido 1513 MSP-1 induce protección contra la malariaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Colecciones
Artículos