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Size polymorphism and low sequence diversity in the locus encoding the Plasmodium vivax rhoptry neck protein 4 (PvRON4) in Colombian isolates

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dc.creatorBuitrago, Sindy P.spa
dc.creatorGarzón-Ospina, Diegospa
dc.creatorPatarroyo, Manuel A.spa
dc.date.accessioned2020-05-25T23:56:52Z
dc.date.available2020-05-25T23:56:52Z
dc.date.created2016spa
dc.description.abstractBackground: Designing a vaccine against Plasmodium vivax has focused on selecting antigens involved in invasion mechanisms that must have domains with low polymorphism for avoiding allele-specific immune responses. The rhoptry neck protein 4 (RON4) forms part of the tight junction, which is essential in the invasion of hepatocytes and/or erythrocytes; however, little is known about this locus’ genetic diversity. Methods: DNA sequences from 73 Colombian clinical isolates from pvron4 gene were analysed for characterizing their genetic diversity; pvron4 haplotype number and distribution, as well as the evolutionary forces determining diversity pattern, were assessed by population genetics and molecular evolutionary approaches. Results: ron4 has low genetic diversity in P. vivax at sequence level; however, a variable amount of tandem repeats at the N-terminal region leads to extensive size polymorphism. This region seems to be exposed to the immune system. The central region has a putative esterase/lipase domain which, like the protein’s C-terminal fragment, is highly conserved at intra- and inter-species level. Both regions are under purifying selection. Conclusions: pvron4 is the locus having the lowest genetic diversity described to date for P. vivax. The repeat regions in the N-terminal region could be associated with immune evasion mechanisms while the central region and the C-terminal region seem to be under functional or structural constraint. Bearing such results in mind, the PvRON4 central and/or C-terminal portions represent promising candidates when designing a subunit-based vaccine as they are aimed at avoiding an allele-specific immune response, which might limit vaccine efficacy. © 2016 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s12936-016-1563-4
dc.identifier.issn14752875
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22548
dc.language.isoengspa
dc.publisherBioMed Central Ltd.spa
dc.relation.citationEndPage12
dc.relation.citationIssueNo. 1
dc.relation.citationStartPage1
dc.relation.citationTitleMalaria Journal
dc.relation.citationVolumeVol. 15
dc.relation.ispartofMalaria Journal, ISSN:14752875, Vol.15, No.1 (2016); pp. 1-12spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84992410774&doi=10.1186%2fs12936-016-1563-4&partnerID=40&md5=97a3e73d5d1a20a73aa3532e3278f3bespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordEsterasespa
dc.subject.keywordmoleculareng
dc.subject.keywordTight junction proteinspa
dc.subject.keywordTriacylglycerol lipasespa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordProtozoal dnaspa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordAmino terminal sequencespa
dc.subject.keywordArticlespa
dc.subject.keywordCarboxy terminal sequencespa
dc.subject.keywordColombiaspa
dc.subject.keywordControlled studyspa
dc.subject.keywordDna sequencespa
dc.subject.keywordGenespa
dc.subject.keywordGenetic conservationspa
dc.subject.keywordGenetic polymorphismspa
dc.subject.keywordGenetic variabilityspa
dc.subject.keywordImmune systemspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordPurifying selectionspa
dc.subject.keywordPvron4 genespa
dc.subject.keywordTandem repeatspa
dc.subject.keywordAdolescentspa
dc.subject.keywordAdultspa
dc.subject.keywordChemistryspa
dc.subject.keywordCluster analysisspa
dc.subject.keywordFemalespa
dc.subject.keywordGenetic variationspa
dc.subject.keywordGeneticsspa
dc.subject.keywordHaplotypespa
dc.subject.keywordHumanspa
dc.subject.keywordIsolation and purificationspa
dc.subject.keywordMalespa
dc.subject.keywordMiddle agedspa
dc.subject.keywordMolecular evolutionspa
dc.subject.keywordParasitologyspa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordPlasmodium vivax malariaspa
dc.subject.keywordSequence homologyspa
dc.subject.keywordYoung adultspa
dc.subject.keywordAdolescentspa
dc.subject.keywordAdultspa
dc.subject.keywordCluster analysisspa
dc.subject.keywordColombiaspa
dc.subject.keywordDnaeng
dc.subject.keywordEvolutioneng
dc.subject.keywordFemalespa
dc.subject.keywordGenetic variationspa
dc.subject.keywordHaplotypesspa
dc.subject.keywordHumansspa
dc.subject.keywordMalariaeng
dc.subject.keywordMalespa
dc.subject.keywordMiddle agedspa
dc.subject.keywordPhylogenyspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordSequence analysiseng
dc.subject.keywordSequence homologyspa
dc.subject.keywordYoung adultspa
dc.subject.keywordFunctional restrictionspa
dc.subject.keywordGenetic diversityspa
dc.subject.keywordNatural selectionspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordPvron4spa
dc.subject.keywordRhoptryspa
dc.subject.keywordTandem repeatspa
dc.titleSize polymorphism and low sequence diversity in the locus encoding the Plasmodium vivax rhoptry neck protein 4 (PvRON4) in Colombian isolatesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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