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A large size chimeric highly immunogenic peptide presents multistage plasmodium antigens as a vaccine candidate system against malaria

dc.creatorLozano, José Manuelspa
dc.creatorVarela, Yahsonspa
dc.creatorSilva, Yolandaspa
dc.creatorArdila, Karenspa
dc.creatorForero, Marthaspa
dc.creatorGuasca, Lauraspa
dc.creatorGuerrero, Yulyspa
dc.creatorBermudez, Adrianaspa
dc.creatorAlba, Patriciaspa
dc.creatorVanegas, Magnoliaspa
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-05-25T23:57:12Z
dc.date.available2020-05-25T23:57:12Z
dc.date.created2017spa
dc.description.abstractRational strategies for obtaining malaria vaccine candidates should include not only a proper selection of target antigens for antibody stimulation, but also a versatile molecular design based on ordering the right pieces from the complex pathogen molecular puzzle towards more active and functional immunogens. Classical Plasmodium falciparum antigens regarded as vaccine candidates have been selected as model targets in this study. Among all possibilities we have chosen epitopes of PfCSP, STARP; MSA1 and Pf155/RESA from pre- A nd erythrocyte stages respectively for designing a large 82-residue chimeric immunogen. A number of options aimed at diminishing steric hindrance for synthetic procedures were assessed based on standard Fmoc chemistry such as building block orthogonal ligation; pseudo-proline and microwave-assisted procedures, therefore the large-chimeric target was produced, characterized and immunologically tested. Antigenicity and functional in vivo efficacy tests of the large-chimera formulations administered alone or as antigen mixtures have proven the stimulation of high antibody titers, showing strong correlation with protection and parasite clearance of vaccinated BALB/c mice after being lethally challenged with both P. berghei-ANKA and P. yoelii 17XL malaria strains. Besides, 3D structure features shown by the large-chimera encouraged as to propose using these rational designed large synthetic molecules as reliable vaccine candidate-presenting systems.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3390/molecules22111837
dc.identifier.issn14203049
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22626
dc.language.isoengspa
dc.publisherMDPI AGspa
dc.relation.citationIssueNo. 11
dc.relation.citationTitleMolecules
dc.relation.citationVolumeVol. 22
dc.relation.ispartofMolecules, ISSN:14203049, Vol.22, No.11 (2017)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85033805074&doi=10.3390%2fmolecules22111837&partnerID=40&md5=5d0b3161ea7a1a1db41e89b2740744a1spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordEpitopespa
dc.subject.keywordprotozoaneng
dc.subject.keywordParasite antigenspa
dc.subject.keywordPeptidespa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordAnimalspa
dc.subject.keywordBagg albino mousespa
dc.subject.keywordImmunologyspa
dc.subject.keywordMalariaspa
dc.subject.keywordMousespa
dc.subject.keywordPathogenicityspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordAnimalsspa
dc.subject.keywordAntigenseng
dc.subject.keywordEpitopesspa
dc.subject.keywordMalariaspa
dc.subject.keywordMalaria vaccinesspa
dc.subject.keywordMicespa
dc.subject.keywordMiceeng
dc.subject.keywordPeptidesspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordChimeric immunogenspa
dc.subject.keywordMacromolecule synthesisspa
dc.subject.keywordMalariaspa
dc.subject.keywordPlasmodiumspa
dc.subject.keywordVaccine candidatespa
dc.titleA large size chimeric highly immunogenic peptide presents multistage plasmodium antigens as a vaccine candidate system against malariaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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