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Recombinant vaccines of a CD4+ T-cell epitope promote efficient control of Paracoccidioides brasiliensis burden by restraining primary organ infection

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dc.creatorAlves Santos, Julliana Ribeiro
dc.creatorAssuncão Holanda, Rodrigo
dc.creatorMuñoz Henao, Julián Esteban
dc.creatorSantos Dias, Lucas
dc.creatorRoque Silva, Leandro Buffoni
dc.creatorAlves Santos, Julliana Ribeiro
dc.creatorPagliari, Sthefany
dc.creatorLeandro, Érica
dc.creatorVieira, Marciano
dc.creatorAlves Paixão, Tatiane
dc.creatorPelleschi Taborda, Carlos
dc.creatorAssis Santos, Daniel
dc.creatorBruña-Romero, Oscar
dc.creator.googleAlves Santos, Julliana Ribeirospa
dc.creator.googleAssuncão Holanda, Rodrigospa
dc.creator.googleMuñoz, Julián Estebanspa
dc.creator.googleSantos Dias, Lucasspa
dc.creator.googleRoque Silva, Leandro Buffonispa
dc.creator.googleAlves Santos, Julliana Ribeirospa
dc.creator.googlePagliari, Sthefanyspa
dc.creator.googleLeandro, Éricaspa
dc.creator.googleVieira, Marcianospa
dc.creator.googleAlves Paixão, Tatianespa
dc.creator.googlePelleschi Taborda, Carlosspa
dc.creator.googleAssis Santos, Danielspa
dc.creator.googleBruña-Romero, Oscarspa
dc.date.accessioned2018-11-06T17:50:12Z
dc.date.available2018-11-06T17:50:12Z
dc.date.created2017
dc.date.issued2017
dc.description.abstractParacoccidioidomycosis (PCM) is an infectious disease endemic to South America, caused by the thermally dimorphic fungi Paracoccidioides. Currently, there is no effective human vaccine that can be used in prophylactic or therapeutic regimes. We tested the hypothesis that the immunogenicity of the immunodominant CD4+T-cell epitope (P10) of Paracoccidioides brasiliensis gp43 antigen might be significantly enhanced by using a hepatitis B virus-derived particle (VLP) as an antigen carrier. This chimera was administered to mice as a (His)6-purified protein (rPbT) or a replication-deficient human type 5 adenoviral vector (rAdPbT) in an immunoprophylaxis assay. The highly virulent Pb18 yeast strain was used to challenge our vaccine candidates. Fungal challenge evoked robust P10-specific memory CD4+T cells secreting protective Th-1 cytokines in most groups of immunized mice. Furthermore, the highest level of fungal burden control was achieved when rAdPbT was inoculated in a homologous prime-boost regimen, with 10-fold less CFU recovering than in non-vaccinated mice. Systemic Pb18 spreading was only prevented when rAdPbT was previously inoculated. In summary, we present here VLP/P10 formulations as vaccine candidates against PCM, some of which have demonstrated for the first time their ability to prevent progression of this pernicious fungal disease, which represents a significant social burden in developing countries. © 2017 Holanda et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0005927
dc.identifier.issnISSN 1935-2727
dc.identifier.issnISSNe 1935-2735
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18682
dc.language.isoengspa
dc.relation.citationIssueNo. 9
dc.relation.citationTitlePLoS Neglected Tropical Diseases
dc.relation.citationVolumeVol. 11
dc.relation.ispartofPLoS Neglected Tropical Diseases, ISSN 1935-2727, Vol. 11 No. 9 (2017)spa
dc.relation.urihttps://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0005927&type=printablespa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/spa
dc.source.bibliographicCitationMunoz, J.F., Gallo, J.E., Misas, E., Priest, M., Imamovic, A., Young, S., Genome update of the dimorphic human pathogenic fungi causing paracoccidioidomycosis (2014) Plos Neglect Trop D, 8, p. e3348spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectEpítopospa
dc.subjectPartícula similar a un virusspa
dc.subjectInterferón gammaspa
dc.subjectHongosspa
dc.subjectSintéticospa
dc.subjectLinfocito Tspa
dc.subjectBalb endogámico Cspa
dc.subjectParacoccidioidesspa
dc.subjectDesarrollo y Envejecimientospa
dc.subjectVacunaspa
dc.subjectInterleucina 1spa
dc.subjectinterleucina 12spa
dc.subjectinterleucina 4spa
dc.subjectVacuna recombinantespa
dc.subjectFactor de necrosis tumoralspa
dc.subjectProteína de 43 Kdaspa
dc.subjectCitocinaspa
dc.subjectEpítopospa
dc.subjectProteína fúngicaspa
dc.subjectAntígeno de hongosspa
dc.subjectVacuna contra hongosspa
dc.subjectGlicoproteínaspa
dc.subjectVacuna recombinantespa
dc.subjectVacuna contra partículas similares a virusspa
dc.subjectCélula animalspa
dc.subjectExperimento con animalesspa
dc.subjectModelo animalspa
dc.subjectTejido animalspa
dc.subjectLinfocito T Cd4+spa
dc.subjectInmunidad celularspa
dc.subjectCentrifugaciónspa
dc.subjectQuimeraspa
dc.subjectUnidad de formación de Coloniaspa
dc.subjectEstudio controladospa
dc.subjectReplicación de ADNspa
dc.subjectFormulación de Medicamentosspa
dc.subjectEnsayo inmunoabsorbente ligado a enzimasspa
dc.subjectHepatitis Bspa
dc.subjectHistopatologíaspa
dc.subjectHumanospa
dc.subjectCélula humanaspa
dc.subjectInmunizaciónspa
dc.subjectInmunofenotipadospa
dc.subjectInmunoprofilaxisspa
dc.subjectProliferación de linfocitosspa
dc.subjectMétodo de estimación de la magnitudspa
dc.subjectMortalidadspa
dc.subjectSíntesis de péptidosspa
dc.subjectPlásmidospa
dc.subjectElectroforesis en gel de poliacrilamidaspa
dc.subjectExpresión de proteínasspa
dc.subjectExtracción de fase sólidaspa
dc.subjectBlastomicosis sudamericanaspa
dc.subjectLesión de tejidospa
dc.subjectMicroscopio de transmisión por electronesspa
dc.subjectInmunogenicidad de la vacunaspa
dc.subjectPartícula de virusspa
dc.subjectTransferencia occidentalspa
dc.subjectRatón albino baggspa
dc.subjectGenéticaspa
dc.subjectCrecimientospa
dc.subjectHepatitis B Virusspa
dc.subjectMemoria Inmunológicaspa
dc.subjectInmunologíaspa
dc.subjectMicrobiologíaspa
dc.subjectParacoccidioidesspa
dc.subjectParacoccidioidomicosisspa
dc.subjectSecreción (Proceso)spa
dc.subjectCélula Th1spa
dc.subject.keywordArticlespa
dc.subject.keywordTh1 Celleng
dc.subject.keywordSecretion (Process)eng
dc.subject.keywordParacoccidioidomycosiseng
dc.subject.keywordMicrobiologyeng
dc.subject.keywordLivereng
dc.subject.keywordImmunologyeng
dc.subject.keywordImmunological Memoryeng
dc.subject.keywordGrowtheng
dc.subject.keywordGeneticseng
dc.subject.keywordWestern Blottingeng
dc.subject.keywordBagg Albino Mouseeng
dc.subject.keywordVirus Particleeng
dc.subject.keywordVaccine Immunogenicityeng
dc.subject.keywordTransmission Electron Microscopyeng
dc.subject.keywordTissue Injuryeng
dc.subject.keywordSouth American Blastomycosiseng
dc.subject.keywordSolid Phase Extractioneng
dc.subject.keywordProtein Expressioneng
dc.subject.keywordPolyacrylamide Gel Electrophoresiseng
dc.subject.keywordPlasmideng
dc.subject.keywordPeptide Synthesiseng
dc.subject.keywordMortalityeng
dc.subject.keywordMagnitude Estimation Methodeng
dc.subject.keywordLymphocyte Proliferationeng
dc.subject.keywordImmunoprophylaxiseng
dc.subject.keywordImmunophenotypingeng
dc.subject.keywordImmunizationeng
dc.subject.keywordHuman Celleng
dc.subject.keywordHumaneng
dc.subject.keywordHistopathologyeng
dc.subject.keywordEnzyme Linked Immunosorbent Assayeng
dc.subject.keywordDrug Formulationeng
dc.subject.keywordDna Replicationeng
dc.subject.keywordControlled Studyeng
dc.subject.keywordColony Forming Uniteng
dc.subject.keywordChimeraeng
dc.subject.keywordCentrifugationeng
dc.subject.keywordCellular Immunityeng
dc.subject.keywordCd4+ T Lymphocyteeng
dc.subject.keywordAnimal Tissueeng
dc.subject.keywordAnimal Modeleng
dc.subject.keywordAnimal Experimenteng
dc.subject.keywordAnimal Celleng
dc.subject.keywordVirus Like Particle Vaccineeng
dc.subject.keywordRecombinant Vaccineeng
dc.subject.keywordGlycoproteineng
dc.subject.keywordFungus Vaccineeng
dc.subject.keywordFungus Antigeneng
dc.subject.keywordFungal Proteineng
dc.subject.keywordEpitopeeng
dc.subject.keywordCytokineeng
dc.subject.keyword43 Kda Proteineng
dc.subject.keywordTumor Necrosis Factoreng
dc.subject.keywordRecombinant Vaccineeng
dc.subject.keywordInterleukin 4eng
dc.subject.keywordInterleukin 12eng
dc.subject.keywordInterleukin 1eng
dc.subject.keywordVaccineeng
dc.subject.keywordDevelopment And Agingeng
dc.subject.keywordParacoccidioideseng
dc.subject.keywordInbred Balb Ceng
dc.subject.keywordT-Lymphocyteeng
dc.subject.keywordSyntheticeng
dc.subject.keywordFungaleng
dc.subject.keywordGamma Interferoneng
dc.subject.keywordVirus-Like Particleeng
dc.subject.keywordEpitopeeng
dc.subject.lembGlucoproteinasspa
dc.subject.lembParacoccidioidomycosisspa
dc.subject.lembMicosisspa
dc.titleRecombinant vaccines of a CD4+ T-cell epitope promote efficient control of Paracoccidioides brasiliensis burden by restraining primary organ infectionspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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