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Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women

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dc.creatorMunoz, Marinaspa
dc.creatorCamargo, Milenaspa
dc.creatorLeon, Sara C. Soto-Despa
dc.creatorSanchez, Ricardospa
dc.creatorPineda-Peña, Andrea C.spa
dc.creatorPerez-Prados, Antoniospa
dc.creatorPatarroyo, Manuel E.spa
dc.creatorPatarroyo, Manuel A.spa
dc.date.accessioned2020-05-25T23:56:02Z
dc.date.available2020-05-25T23:56:02Z
dc.date.created2013spa
dc.description.abstractHuman papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness.This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5-/GP6-, MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women.Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11).HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples.HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples.HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in rine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]).It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples.This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples.© 2013, American Society for Microbiology.All Rights Reserved.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1128/JCM.01302-13
dc.identifier.issn1098660X
dc.identifier.issn00951137
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22302
dc.language.isoengspa
dc.relation.citationEndPage3693
dc.relation.citationIssueNo. 11
dc.relation.citationStartPage3688
dc.relation.citationTitleJournal of Clinical Microbiology
dc.relation.citationVolumeVol. 51
dc.relation.ispartofJournal of Clinical Microbiology, ISSN:1098660X, 00951137, Vol.51, No.11 (2013); pp. 3688-3693spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84886452411&doi=10.1128%2fJCM.01302-13&partnerID=40&md5=b937dc948296cbbbe847a2ad3e27ab74spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordVirus DNAspa
dc.subject.keywordAdultspa
dc.subject.keywordArticlespa
dc.subject.keywordCancer screeningspa
dc.subject.keywordControlled studyspa
dc.subject.keywordFemalespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman immunodeficiency virus infectionspa
dc.subject.keywordHuman papillomavirus type 11spa
dc.subject.keywordHuman papillomavirus type 16spa
dc.subject.keywordHuman papillomavirus type 18spa
dc.subject.keywordHuman papillomavirus type 31spa
dc.subject.keywordHuman papillomavirus type 33spa
dc.subject.keywordHuman papillomavirus type 45spa
dc.subject.keywordHuman papillomavirus type 58spa
dc.subject.keywordHuman papillomavirus type 6spa
dc.subject.keywordMajor clinical studyspa
dc.subject.keywordMixed infectionspa
dc.subject.keywordNonhumanspa
dc.subject.keywordPapillomavirus infectionspa
dc.subject.keywordPolymerase chain reactionspa
dc.subject.keywordPrevalencespa
dc.subject.keywordPriority journalspa
dc.subject.keywordUrinalysisspa
dc.subject.keywordUterine cervix cytologyspa
dc.subject.keywordVirus detectionspa
dc.subject.keywordWart virusspa
dc.subject.keywordAdultspa
dc.subject.keywordCervix Uterispa
dc.subject.keywordDNA Primersspa
dc.subject.keywordDNAeng
dc.subject.keywordFemalespa
dc.subject.keywordHIV Infectionsspa
dc.subject.keywordHumansspa
dc.subject.keywordMiddle Agedspa
dc.subject.keywordMolecular Diagnostic Techniquesspa
dc.subject.keywordPapillomaviridaespa
dc.subject.keywordPapillomavirus Infectionsspa
dc.subject.keywordPolymerase Chain Reactionspa
dc.subject.keywordSensitivity and Specificityspa
dc.subject.keywordUrinespa
dc.subject.keywordVirologyspa
dc.titleClassical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected womenspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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