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Receptor–ligand and parasite protein–protein interactions in Plasmodium vivax: Analysing rhoptry neck proteins 2 and 4

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dc.creatorBermúdez M.spa
dc.creatorArévalo-Pinzón G.spa
dc.creatorRubio L.spa
dc.creatorChaloin O.spa
dc.creatorMuller S.spa
dc.creatorCurtidor H.spa
dc.creatorPatarroyo M.A.spa
dc.date.accessioned2020-05-26T00:04:00Z
dc.date.available2020-05-26T00:04:00Z
dc.date.created2018spa
dc.description.abstractElucidating receptor–ligand and protein–protein interactions represents an attractive alternative for designing effective Plasmodium vivax control methods. This article describes the ability of P. vivax rhoptry neck proteins 2 and 4 (RON2 and RON4) to bind to human reticulocytes. Biochemical and cellular studies have shown that two PvRON2- and PvRON4-derived conserved regions specifically interact with protein receptors on reticulocytes marked by the CD71 surface transferrin receptor. Mapping each protein fragment's binding region led to defining the specific participation of two 20 amino acid-long regions selectively competing for PvRON2 and PvRON4 binding to reticulocytes. Binary interactions between PvRON2 (ligand) and other parasite proteins, such as PvRON4, PvRON5, and apical membrane antigen 1 (AMA1), were evaluated and characterised by surface plasmon resonance. The results revealed that both PvRON2 cysteine-rich regions strongly interact with PvAMA1 Domains II and III (equilibrium constants in the nanomolar range) and at a lower extent with the complete PvAMA1 ectodomain and Domains I and II. These results strongly support that these proteins participate in P. vivax's complex invasion process, thus providing new pertinent targets for blocking P. vivax merozoites' specific entry to their target cells. © 2018 John Wiley and Sons Ltdeng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/cmi.12835
dc.identifier.issn14625814
dc.identifier.issn14625822
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23647
dc.language.isoengspa
dc.publisherBlackwell Publishing Ltdspa
dc.relation.citationIssueNo. 7
dc.relation.citationTitleCellular Microbiology
dc.relation.citationVolumeVol. 20
dc.relation.ispartofCellular Microbiology, ISSN:14625814, 14625822, Vol.20, No.7 (2018)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85044402275&doi=10.1111%2fcmi.12835&partnerID=40&md5=328fd6c535b82ce1b560416ac33ee8c0spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordApical membrane antigen 1spa
dc.subject.keywordCd71 antigenspa
dc.subject.keywordCysteinespa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordRhoptry neck protein 2spa
dc.subject.keywordRhoptry neck protein 4spa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordCd71 antigenspa
dc.subject.keywordLeukocyte antigenspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordTransferrin receptorspa
dc.subject.keywordArticlespa
dc.subject.keywordCarboxy terminal sequencespa
dc.subject.keywordCos-7 cell linespa
dc.subject.keywordErythrocytespa
dc.subject.keywordHumanspa
dc.subject.keywordHuman cellspa
dc.subject.keywordMerozoitespa
dc.subject.keywordNonhumanspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordPriority journalspa
dc.subject.keywordProtein protein interactionspa
dc.subject.keywordReticulocytespa
dc.subject.keywordSurface plasmon resonancespa
dc.subject.keywordUmbilical cord bloodspa
dc.subject.keywordCell adhesionspa
dc.subject.keywordHost pathogen interactionspa
dc.subject.keywordMetabolismspa
dc.subject.keywordParasitologyspa
dc.subject.keywordPhysiologyspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordProtein analysisspa
dc.subject.keywordAntigenseng
dc.subject.keywordCell adhesionspa
dc.subject.keywordHost-pathogen interactionsspa
dc.subject.keywordHumansspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein interaction mappingspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordReceptorseng
dc.subject.keywordReticulocytesspa
dc.subject.keywordSurface plasmon resonancespa
dc.subject.keywordMalariaspa
dc.subject.keywordPlasmodium vivaxspa
dc.subject.keywordReticulocytesspa
dc.subject.keywordRhoptry neck proteinsspa
dc.subject.keywordSynthetic peptidespa
dc.titleReceptor–ligand and parasite protein–protein interactions in Plasmodium vivax: Analysing rhoptry neck proteins 2 and 4spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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