Ítem
Acceso Abierto

TeiR, a LuxR-Type transcription factor required for testosterone degradation in comamonas testosteroni

Mostrar el registro sencillo de la publicación
dc.creatorPruneda-Paz, José Luisspa
dc.creatorLinares, Mauriciospa
dc.creatorCabrera, Julio E.spa
dc.creatorGenti-Raimondi, Susanaspa
dc.date.accessioned2020-08-19T14:40:47Z
dc.date.available2020-08-19T14:40:47Z
dc.date.created2004-02-18spa
dc.description.abstractWe have identified a new steroid-inducible gene (designated teiR [testosterone-inducible regulator]) in Comamonas testosteroni that is required for testosterone degradation. Nucleotide sequence analysis of teiR predicts a 391-amino-acid protein which shows homology between residues 327 and 380 (C-terminal domain) to the LuxR helix-turn-helix DNA binding domain and between residues 192 and 227 to the PAS sensor domain. This domain distribution resembles that described for TraR, a specific transcriptional regulator involved in quorum sensing in Agrobacterium tumefaciens. Analysis of the gene expression indicated that teiR is tightly controlled at the transcriptional level by the presence of testosterone in the culture medium. A teiR-disrupted mutant strain was completely unable to use testosterone as the sole carbon and energy source. In addition, the expression of several steroid-inducible genes was abolished in this mutant. Northern blot assays revealed that teiR is required for full expression of sip48–-HSD gene mRNA (encoding a steroid-inducible protein of 48 kDa and 3-17-hydroxysteroid dehydrogenase) and also of other steroid degradation genes, including those encoding 3-hydroxysteroid dehydrogenase, 5 –3-ketoisomerase, 3-oxo-steroid 1 -dehydrogenase, and 3-oxosteroid 4 -(5)-dehydrogenase enzymes. Moreover, when teiR was provided to the teiR-disrupted strain in trans, the transcription level of these genes was restored. These results indicate that TeiR positively regulates the transcription of genes involved in the initial enzymatic steps of steroid degradation in C. testosteroni.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1128/JB.186.5.1430-1437.2004
dc.identifier.issnISSN: 0021-9193
dc.identifier.issnEISSN: 1098-5530
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/27025
dc.language.isoengspa
dc.publisherAmerican Society for Microbiologyspa
dc.relation.citationEndPage1437
dc.relation.citationIssueNo. 5
dc.relation.citationStartPage1430
dc.relation.citationTitleJournal of Bacteriology
dc.relation.citationVolumeVol. 185
dc.relation.ispartofJournal of Bacteriology, ISSN: 0021-9193;EISSN: 1098-5530, Vol.185, No.5 (March 2004); pp. 1430-1437spa
dc.relation.urihttps://jb.asm.org/content/jb/186/5/1430.full.pdfspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceJournal of Bacteriologyspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordAndrogens metabolismspa
dc.subject.keywordBacterial proteins metabolismspa
dc.subject.keywordComamonas testosteroni metabolismspa
dc.subject.keywordGene expression regulationspa
dc.subject.keywordbacterialspa
dc.subject.keywordRepressor proteins metabolismspa
dc.subject.keywordTestosterone metabolismspa
dc.subject.keywordTrans-activators metabolismspa
dc.titleTeiR, a LuxR-Type transcription factor required for testosterone degradation in comamonas testosteronispa
dc.title.TranslatedTitleTeiR, un factor de transcripción tipo LuxR necesario para la degradación de la testosterona en comamonas testosteronispa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
Journal-of-Bacteriology-2004-Pruneda-Paz-1430-full.pdf
Tamaño:
527.68 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos