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Molecular diagnosis of trypanosomatids in Didelphis marsupialis reveals risk areas for Trypanosoma cruzi transmission and sympatric circulation with T. rangeli in the metropolitan area of Bucaramanga, Santander, Colombia

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dc.creatorRamírez González, Juan Davidspa
dc.creatorMurcia-Cueto, Ian Sebastianspa
dc.creatorJiménez-Leano, Angela Patriciaspa
dc.creatorOspina, Carlos M.spa
dc.creatorPatino, Luz H.spa
dc.creatorArdila-Gélvez, Andreaspa
dc.creatorQuintero-Sanchez, Vladimirspa
dc.creatorJaimes-Duenez, Jeiczon Elimspa
dc.date.accessioned2025-07-21T16:49:53Z
dc.date.available2025-07-21T16:49:53Z
dc.date.created2025-08-01spa
dc.date.issued2025-08-01spa
dc.description.abstractThe adaptation of wild animals to urban environments can lead to increased contact with humans and a higher risk of exposure to zoonotic agents. Didelphis marsupialis (common opossum) is an important reservoir of Trypanosoma cruzi and Leishmania spp., which commonly affect human populations in Latin America. Therefore, this study aimed to assess the frequency of trypanosomatid infections and characterize T. cruzi DTUs in common opossums from the Metropolitan Area of Bucaramanga (MAB), Santander, Colombia. A total of 70 individuals from four municipalities (Bucaramanga, Floridablanca, Giron, ´ and Piedecuesta) were analyzed by PCR using blood samples, of which 14.3 % (95 % CI: 7.95–24.3 %) tested positive for trypanosomatids. Next-generation sequencing of 18S and Hsp70 genes in positive samples identified T. cruzi DTU TcI and T. rangeli in nine (12.9 %, 95 % CI: 6.91–22.66 %) and two (2.86 %, 95 % CI: 0.79–9.83 %) samples, respectively, including one case of co-infection (1.43 %, 95 % CI: 0.04–7.7 %). A heatmap revealed a high concentration of T. cruzi-positive cases in peripheral neighborhoods of Bucaramanga adjacent to forested areas. This study confirms the presence of an enzootic transmission cycle of T. cruzi in the MAB, highlighting the role of D. marsupialis as an important reservoir, particularly in peripheral neighborhoods of Bucaramanga. The sympatric circulation of T. cruzi and T. rangeli in opossums from the MAB introduces new epidemiological challenges for Chagas disease control in these areas, emphasizing the need for improved diagnostic strategies to differentiate both parasites in patients and epidemiological studies including vectors and reservoirs.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1016/j.ijppaw.2025.101072spa
dc.identifier.issn2213-2244spa
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/46090
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.ispartofInternational Journal for Parasitology: Parasites and Wildlife Volume 27, August 2025spa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S2213224425000379?via%3Dihubspa
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccessspa
dc.rights.accesoAbierto (Texto Completo)spa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourceInternational Journal for Parasitology: Parasites and Wildlifespa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordChagas diseaseseng
dc.subject.keywordLeishmaniasiseng
dc.subject.keywordNext-generation sequencingeng
dc.subject.keywordCoinfectioneng
dc.subject.keywordReservoireng
dc.titleMolecular diagnosis of trypanosomatids in Didelphis marsupialis reveals risk areas for Trypanosoma cruzi transmission and sympatric circulation with T. rangeli in the metropolitan area of Bucaramanga, Santander, Colombiaspa
dc.typearticlespa
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersionspa
dc.type.spaArtículo de Investigaciónspa
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