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In vitro kinetics of reticulocyte subtypes : maturation after red blood cell storage in additive solution-1 (AS-1)

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dc.creatorUrbina Bonilla, Adriana del Pilar
dc.creatorPalomino, Fernando
dc.creator.googleUrbina, Adrianaspa
dc.creator.googlePalomino, Fernando
dc.date.accessioned2019-10-01T15:22:01Z
dc.date.available2019-10-01T15:22:01Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractBackground: Reticulocytes are immature red blood cells containing RNA remnants. Their population kinetics has been documented under various in vivo and in vitro conditions, including after storage of red blood cells in blood banks. The purpose of this study was to describe the influence of blood bank storage on the kinetics of reticulocyte disappearance by in vitro culturing. Method: Samples of reticulocyte-enriched fractions (Percoll density-gradient) were obtained over different storage times from six red blood cell units stored in additive solution-1 (AS-1). Reticulocyte fractions were then cultured in enriched media at 37 °C and analyzed by flow cytometry with thiazole orange taking into account hemolysis. Results: Density-gradient enriched reticulocyte fractions were obtain from standard red blood cell units with <1% of reticulocytes. An exponential drop of reticulocytes was observed in cultures. The time taken for reticulocyte disappearance in cultures was shorter with increased blood bank storage time (144 ± 46 h at 0.5 weeks of storage and 15 ± 14 h in the sixth week). High fluorescence reticulocytes disappeared completely in 42.5 ± 8.5 h, medium fluorescence reticulocytes in 73.4 ± 20.8 h and low fluorescence reticulocytes in 269.9 ± 98.8 h in red blood cell units stored for half a week. These times significantly decreased in red blood cell units stored for more time. Conclusion: In vitro reticulocyte disappearance was significantly faster after prolonged storage of red blood cell units at 4 °C. The in vitro half-life at 0.5 weeks of storage was not significantly different from the values reported for fresh venous blood, but after the sixth week of storage, the half-lives were shorter. The possible explanation is that blood bank storage does not cause irreversible damage to the human reticulocyte maturational machinery. © 2018 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celulareng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1016/j.htct.2017.12.002
dc.identifier.issn2531-1379
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/20373
dc.language.isoengspa
dc.relation.citationEndPage150
dc.relation.citationStartPage143
dc.relation.citationTitleHematology Transfusion and Cell Therapy
dc.relation.citationVolumeVol. 40
dc.relation.ispartofHematology, Transfusion and Cell Therapy, ISSN:2531-1379, Vol. 40 (2018) pp. 143-150spa
dc.relation.urihttp://www.scielo.br/pdf/htct/v40n2/2531-1379-htct-40-02-0143.pdfspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitationKoury, M.J., Koury, S.T., Kopsombut, P., Bondurant, M.C., In vitro maturation of nascent reticulocytes to erythrocytes (2005) Blood, 105 (5), pp. 2168-2174spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subjectBovine Serum Albuminspa
dc.subjectBufferspa
dc.subjectGlucosespa
dc.subjectHemoglobinspa
dc.subjectThiazole Orangespa
dc.subjectAlgorithmspa
dc.subjectAqueous Solutionspa
dc.subjectBlood Bankspa
dc.subjectCell Culturespa
dc.subjectCell Fractionationspa
dc.subjectCell Isolationspa
dc.subjectCell Kineticsspa
dc.subjectCell Maturationspa
dc.subjectCell Survivalspa
dc.subjectControlled Studyspa
dc.subjectDensity Gradientspa
dc.subjectErythrocyte Countspa
dc.subjectErythrocyte Preservationspa
dc.subjectFlow Cytometryspa
dc.subjectFluorescence Analysisspa
dc.subjectHalf Life Timespa
dc.subjectHemolysisspa
dc.subjectHumanspa
dc.subjectKinetic Parametersspa
dc.subjectLight Scatteringspa
dc.subjectMathematical Analysisspa
dc.subjectNormal Humanspa
dc.subjectReticulocytespa
dc.subjectSpectrophotometryspa
dc.subjectAlbúmina de suero bovinospa
dc.subjectBufferspa
dc.subjectGlucosaspa
dc.subject.ddcFisiología humanaspa
dc.subject.keywordReticulocyte maturationspa
dc.subject.keywordBlood bankspa
dc.subject.keywordRed-blood-cell unitspa
dc.subject.keywordHemoglobinaspa
dc.subject.keywordArticlespa
dc.subject.lembGlucosaspa
dc.subject.lembHemoglobinaspa
dc.subject.lembReticulocitosspa
dc.titleIn vitro kinetics of reticulocyte subtypes : maturation after red blood cell storage in additive solution-1 (AS-1)spa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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