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A Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies

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dc.audienceComunidad Rosaristaspa
dc.creatorBlomqvist, Karin
dc.creatorAlbrecht, Letusa
dc.creatorQuintana, Maria del Pilar
dc.creatorAngeletti, Davide
dc.creatorJoannin, Nicolas
dc.creatorChêne, Arnaud
dc.creatorMoll, Kirsten
dc.creatorWahlgren, Mats
dc.creator.googleBlomqvist, Karin
dc.creator.googleAlbrecht, Letusa
dc.creator.googleQuintana, Maria del Pilar
dc.creator.googleAngeletti, Davide
dc.creator.googleJoannin, Nicolas
dc.creator.googleChêne, Arnaud
dc.creator.googleMoll, Kirsten
dc.creator.googleWahlgren, Mats
dc.date.accessioned2014-08-11T14:48:06Z
dc.date.available2014-08-11T14:48:06Z
dc.date.created2013-01-15
dc.date.issued2013
dc.description.abstractImmunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1a previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1a-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1a antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surfaceeng
dc.format.mediumRecurso electrónicospa
dc.format.mimetypeapplication/pdf
dc.format.tipoDocumentospa
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0052679
dc.identifier.issn1932-6203
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/8765
dc.language.isoeng
dc.publisherUniversidad del Rosariospa
dc.relation.citationIssueNo. 1
dc.relation.citationTitlePLOS ONE
dc.relation.citationVolumeVol. 8
dc.relation.ispartofPLOS ONE, ISSN 1932-6203, V. 8 N. 1 Jan, 2013spa
dc.relation.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052679
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto completo)spa
dc.rights.licenciaEL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.decsMalariaspa
dc.subject.decsInmunologíaspa
dc.subject.decsPfEMP1spa
dc.subject.decsPlasmodiumspa
dc.subject.keywordErythrocyte membrane protein 1eng
dc.subject.keywordprotozoaneng
dc.subject.keywordProtein antibodyeng
dc.subject.keywordMoleculareng
dc.subject.keywordPreschooleng
dc.subject.keywordStrain transcending antibodyeng
dc.subject.keywordUnclassified drugeng
dc.subject.keywordAmino acid sequenceeng
dc.subject.keywordAntigen antibody reactioneng
dc.subject.keywordAntigen bindingeng
dc.subject.keywordChildeng
dc.subject.keywordDisease severityeng
dc.subject.keywordErythrocyteeng
dc.subject.keywordFlow cytometryeng
dc.subject.keywordImmunofluorescence testeng
dc.subject.keywordIn vitro studyeng
dc.subject.keywordPlasmodium falciparumeng
dc.subject.keywordPreschool childeng
dc.subject.keywordProtein bindingeng
dc.subject.keywordProtein domaineng
dc.subject.keywordProtein motifeng
dc.subject.keywordAmino acid motifseng
dc.subject.keywordErythrocyteseng
dc.subject.keywordImmunoglobulin geng
dc.subject.keywordMolecular sequence dataeng
dc.subject.keywordPlasmodium falciparumeng
dc.subject.keywordPlasmodium falciparumeng
dc.titleA Sequence in Subdomain 2 of DBL1a of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodiesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersion
dc.type.spaArtículospa
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