Ítem
Acceso Abierto

Molecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombia

Mostrar el registro sencillo de la publicación
dc.creatorSánchez, Claudia
dc.creatorLópez, Myriam Consuelo
dc.creatorGaleano, Luis Alejandro
dc.creatorQvarnstrom, Yvonne
dc.creatorHoughton, Katelyn
dc.creatorRamírez, Juan David
dc.creator.googleClaudia Sánchezspa
dc.creator.googleMyriam Consuelo Lópezspa
dc.creator.googleLuis Alejandro Galeanospa
dc.creator.googleYvonne Qvarnstromspa
dc.creator.googleKatelyn Houghtonspa
dc.creator.googleJuan David Ramírezspa
dc.date.accessioned2019-01-11T14:14:17Z
dc.date.available2019-01-11T14:14:17Z
dc.date.created2018
dc.date.issued2018
dc.description.abstractBackground: Protozoan parasites such as Giardia duodenalis, Cryptosporidium spp., Cyclospora cayetanensis, Toxoplasma gondii and Entamoeba histolytica represent a great challenge to the systems producing water for human consumption because their cystic forms are persistent in the environment and resist to the disinfection methods conventionally used for their control. In this study, we investigated the presence of these protozoan pathogens in both raw and treated water samples used for the production of drinking water in Nariño Department, southwest Colombia. We collected 110 water samples (10 lof each sample) and analyzed them with real-time PCR (qPCR). qPCR-positive samples were genotyped with PCR and DNA sequencing. Results: Giardia duodenalis was detected in 35/110 (31.8%) of the samples and Cryptosporidium spp. in 9/110 (8.2%) of the samples; no sample was positive for T. gondii, E. histolytica or C. cayetanensis. Giardia duodenalis was detected in samples of both raw water (Drinking Water Treatment Plants (DWTP): 47.83%;Drinking Water Rural Plants (DWRP): 18.42%) and water collected either after conventional physicochemical treatment (26.09%) or after disinfection by chlorine (50%), whereas Cryptosporidium spp. were only detected in raw waters (DWTP: 17.39%; DWRP: 13.16%). The two pathogens were detected in both types of treatment plants supplying water to urban areas and to rural zones. Analysis of gdh and tpi markers identified assemblages AI, AII and H of G. duodenalis, while analysis of the small subunit rRNA and gp60 markers of Cryptosporidium-positive samples identified C. parvum (Subtype IIcA5G3c), C. galli, C. molnari, Cryptosporidium sp. genotype II of bats and Cryptosporidium sp. genotype VIII of birds. Conclusions: The results obtained demonstrate the presence of protozoan parasites in the water of the study region, and the need to improve the surveillance systems for these pathogens and identify the corresponding sources of contamination. © 2018 The Author(s).eng
dc.format.mimetypeapplication/pdf
dc.identifier.doi10.1186/s13071-018-3147-3
dc.identifier.issnISSN 1756-3305
dc.identifier.urihttp://repository.urosario.edu.co/handle/10336/18858
dc.language.isoengspa
dc.relation.citationEndPage11
dc.relation.citationStartPage2
dc.relation.citationTitleParasites and Vectors
dc.relation.citationVolumeVol. 11
dc.relation.ispartofParasites and Vectors, ISSN:1756-3305, Vol. 11 (2018) pp. 2-11spa
dc.relation.urihttps://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-018-3147-3spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.bibliographicCitationUN-Water Technical Advisory Unit (2014) Annual Report 2014, , UN-Water Genève Switzerland http://www.unwater.org/publications/un-water-annual-report-2014/spa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordDrinking Waterspa
dc.subject.keywordGiardia Duodenalisspa
dc.subject.keywordCryptosporidium Sppspa
dc.subject.keywordDuodenalis Detectedspa
dc.subject.lembAgua potablespa
dc.subject.lembPCR (Bioquímica)spa
dc.titleMolecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombiaspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
Archivos
Bloque original
Mostrando1 - 1 de 1
Miniatura
Nombre:
10.pdf
Tamaño:
1.09 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Colecciones
Artículos