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Strategies for developing multi-epitope, subunit-based, chemically synthesized anti-malarial vaccines

dc.creatorPatarroyo, M. E.spa
dc.creatorCifuentes, G.spa
dc.creatorBermúdez, A.spa
dc.creatorPatarroyo, M. A.spa
dc.date.accessioned2020-08-19T14:40:42Z
dc.date.available2020-08-19T14:40:42Z
dc.date.created2008-10-30spa
dc.description.abstractIntroduction•P. falciparum invasion of RBCs•Merozoite proteins involved in invading erythrocytes•Erythrocyte proteins involved in merozoite invasionThe state of current worldwide anti?malarial vaccine approaches • A rational approach towards developing subunit?based synthetic vaccines • The immune response elicited by conserved HABPs • Structural analysis of native and modified HABPs • Secondary structure analysis • Native and modified HABP 3D structure explains some immunological phenomena • Supporting the haplotype – and allele?conscious TCR concept • Modified HABPs' 3D structure revealed a fit into HLA molecules • Conclusion Abstract An anti?malarial vaccine against the extremely lethal Plasmodium falciparum is desperately needed. Peptides from this parasite's proteins involved in invasion and having high red blood cell?binding ability were identified; these conserved peptides were not immun genic or protection?inducing when used for immunizing Aotus monkeys. Modifying some critical binding residues in these high?activi binding peptides' (HABPs') attachment to red blood cells (RBC) allowed them to induce immunogenicity and protection against expermental challenge and acquire the ability to bind to specific HLA?DRp1* alleles. These modified HABPs adopted certain characterist structural configurations as determined by circular dichroism (CD) and 1H nuclear magnetic resonance (NMR) associated with certain HLA?DR?1* haplotype binding activities and characteristics, such as a 2?Å?distance difference between amino acids fitting into HLA?DRp1 Pockets 1 to 9, residues participating in binding to HLA?DR pockets and residues making contact with the TCR, suggesting haplotyp and allele?conscious TCR. This has been demonstrated in HLA?DR?like genotyped monkeys and provides the basis for designing high effective, subunit?based, multi?antigen, multi?stage, synthetic vaccines, for immediate human use, malaria being one of them.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/j.1582-4934.2008.00174.x
dc.identifier.issnISSN: 1582-1838
dc.identifier.issnEISSN: 1582-4934
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/26996
dc.language.isoengspa
dc.publisherFoundation for Cellular and Molecular Medicinespa
dc.publisherJohn Wiley & Sonsspa
dc.relation.citationEndPage1935
dc.relation.citationIssueNo. 5B
dc.relation.citationStartPage1915
dc.relation.citationTitleJournal of Cellular and Molecular Medicine
dc.relation.citationVolumeVol. 12
dc.relation.ispartofJournal of Cellular and Molecular Medicine, ISSN: 1582-1838;EISSN: 1582-4934, Vol.12, No.5B (October 2008); pp. 1915-1935spa
dc.relation.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1582-4934.2008.00174.xspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.sourceJournal of Cellular and Molecular Medicinespa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordAnti?malarial vaccinespa
dc.subject.keywordP. falciparumspa
dc.subject.keywordHLA?DR?1* moleculesspa
dc.titleStrategies for developing multi-epitope, subunit-based, chemically synthesized anti-malarial vaccinesspa
dc.title.TranslatedTitleEstrategias para desarrollar vacunas antipalúdicas sintetizadas químicamente, basadas en subunidades y multiepítoposspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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