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Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability

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dc.creatorAvendaño C.spa
dc.creatorJenkins M.spa
dc.creatorMéndez-Callejas G.spa
dc.creatorOviedo J.spa
dc.creatorGuzmán F.spa
dc.creatorPatarroyo M.A.spa
dc.creatorSánchez-Acedo C.spa
dc.creatorQuílez J.spa
dc.date.accessioned2020-05-26T00:02:14Z
dc.date.available2020-05-26T00:02:14Z
dc.date.created2018spa
dc.description.abstractCryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended. © 2018 Elsevier Ltdeng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.vaccine.2018.09.044
dc.identifier.issn0264410X
dc.identifier.issn13588745
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/23462
dc.language.isoengspa
dc.publisherElsevier Ltdspa
dc.relation.citationEndPage6710
dc.relation.citationIssueNo. 45
dc.relation.citationStartPage6703
dc.relation.citationTitleVaccine
dc.relation.citationVolumeVol. 36
dc.relation.ispartofVaccine, ISSN:0264410X, 13588745, Vol.36, No.45 (2018); pp. 6703-6710spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85053915548&doi=10.1016%2fj.vaccine.2018.09.044&partnerID=40&md5=99a8717593b22d1e9b7987722393073dspa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordCircumsporozoite like antigen vaccinespa
dc.subject.keywordFreund adjuvantspa
dc.subject.keywordNeutralizing antibodyspa
dc.subject.keywordPeptide vaccinespa
dc.subject.keywordProtozoal vaccinespa
dc.subject.keywordSodium chloridespa
dc.subject.keywordSporozoite surface antigen 15 vaccinespa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordParasite antigenspa
dc.subject.keywordPeptidespa
dc.subject.keywordProtozoal dnaspa
dc.subject.keywordProtozoal proteinspa
dc.subject.keywordProtozoal vaccinespa
dc.subject.keywordProtozoon antibodyspa
dc.subject.keywordAnimal experimentspa
dc.subject.keywordAntibody detectionspa
dc.subject.keywordAntibody productionspa
dc.subject.keywordAntibody responsespa
dc.subject.keywordArticlespa
dc.subject.keywordBioinformaticsspa
dc.subject.keywordCell culturespa
dc.subject.keywordControlled studyspa
dc.subject.keywordCryptosporidiosisspa
dc.subject.keywordCryptosporidium parvumspa
dc.subject.keywordDrug formulationspa
dc.subject.keywordDrug synthesisspa
dc.subject.keywordEnzyme linked immunosorbent assayspa
dc.subject.keywordFemalespa
dc.subject.keywordHumoral immunityspa
dc.subject.keywordImmunological proceduresspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordIn vivo studyspa
dc.subject.keywordMousespa
dc.subject.keywordNonhumanspa
dc.subject.keywordNucleotide sequencespa
dc.subject.keywordPeptide analysisspa
dc.subject.keywordPriority journalspa
dc.subject.keywordSeroneutralization testspa
dc.subject.keywordSwiss webster mousespa
dc.subject.keywordVaccine immunogenicityspa
dc.subject.keywordChemistryspa
dc.subject.keywordImmunologyspa
dc.subject.keywordSynthesisspa
dc.subject.keywordAntibodieseng
dc.subject.keywordAntigenseng
dc.subject.keywordCryptosporidium parvumspa
dc.subject.keywordDnaeng
dc.subject.keywordPeptidesspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordProtozoan vaccinesspa
dc.subject.keywordCp15spa
dc.subject.keywordCryptosporidiosisspa
dc.subject.keywordCryptosporidiumspa
dc.subject.keywordCslspa
dc.subject.keywordPeptidespa
dc.subject.keywordVaccinespa
dc.titleCryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capabilityspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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