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A Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodies

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dc.creatorBlomqvist, Karinspa
dc.creatorAlbrecht, Letusaspa
dc.creatordel Pilar Quintana, Mariaspa
dc.creatorAngeletti, Davidespa
dc.creatorJoannin, Nicolasspa
dc.creatorChêne, Arnaudspa
dc.creatorMoll, Kirstenspa
dc.creatorWahlgren, Matsspa
dc.date.accessioned2020-05-25T23:58:19Z
dc.date.available2020-05-25T23:58:19Z
dc.date.created2013spa
dc.description.abstractImmunity to severe malaria is the first level of immunity acquired to Plasmodium falciparum. Antibodies to the variant antigen PfEMP1 (P. falciparum erythrocyte membrane protein 1) present at the surface of the parasitized red blood cell (pRBC) confer protection by blocking microvascular sequestration. Here we have generated antibodies to peptide sequences of subdomain 2 of PfEMP1-DBL1? previously identified to be associated with severe or mild malaria. A set of sera generated to the amino acid sequence KLQTLTLHQVREYWWALNRKEVWKA, containing the motif ALNRKE, stained the live pRBC. 50% of parasites tested (7/14) were positive both in flow cytometry and immunofluorescence assays with live pRBCs including both laboratory strains and in vitro adapted clinical isolates. Antibodies that reacted selectively with the sequence REYWWALNRKEVWKA in a 15-mer peptide array of DBL1?-domains were also found to react with the pRBC surface. By utilizing a peptide array to map the binding properties of the elicited anti-DBL1? antibodies, the amino acids WxxNRx were found essential for antibody binding. Complementary experiments using 135 degenerate RDSM peptide sequences obtained from 93 Ugandan patient-isolates showed that antibody binding occurred when the amino acids WxLNRKE/D were present in the peptide. The data suggests that the ALNRKE sequence motif, associated with severe malaria, induces strain-transcending antibodies that react with the pRBC surface. © 2013 Blomqvist et al.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0052679
dc.identifier.issn19326203
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/22840
dc.language.isoengspa
dc.relation.citationIssueNo. 1
dc.relation.citationTitlePLoS ONE
dc.relation.citationVolumeVol. 8
dc.relation.ispartofPLoS ONE, ISSN:19326203, Vol.8, No.1 (2013)spa
dc.relation.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84872406445&doi=10.1371%2fjournal.pone.0052679&partnerID=40&md5=2d29dbe442d2130574d0921d247d2bd1spa
dc.rights.accesRightsinfo:eu-repo/semantics/openAccess
dc.rights.accesoAbierto (Texto Completo)spa
dc.source.instnameinstname:Universidad del Rosariospa
dc.source.reponamereponame:Repositorio Institucional EdocURspa
dc.subject.keywordErythrocyte membrane protein 1spa
dc.subject.keywordmoleculareng
dc.subject.keywordStrain transcending antibodyspa
dc.subject.keywordUnclassified drugspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAntigen antibody reactionspa
dc.subject.keywordAntigen bindingspa
dc.subject.keywordArticlespa
dc.subject.keywordChildspa
dc.subject.keywordDisease severityspa
dc.subject.keywordErythrocytespa
dc.subject.keywordFemalespa
dc.subject.keywordFlow cytometryspa
dc.subject.keywordHumanspa
dc.subject.keywordImmunofluorescence testspa
dc.subject.keywordIn vitro studyspa
dc.subject.keywordMalariaspa
dc.subject.keywordMalespa
dc.subject.keywordNonhumanspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordPreschool childspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein domainspa
dc.subject.keywordProtein motifspa
dc.subject.keywordAmino acid motifsspa
dc.subject.keywordAmino acid sequencespa
dc.subject.keywordAnimalsspa
dc.subject.keywordAntibodieseng
dc.subject.keywordAntibody specificityspa
dc.subject.keywordAntigenseng
dc.subject.keywordChildeng
dc.subject.keywordCross reactionsspa
dc.subject.keywordEpitopesspa
dc.subject.keywordErythrocytesspa
dc.subject.keywordFemalespa
dc.subject.keywordHumansspa
dc.subject.keywordImmunoglobulin gspa
dc.subject.keywordInfantspa
dc.subject.keywordMalespa
dc.subject.keywordModelseng
dc.subject.keywordMolecular sequence dataspa
dc.subject.keywordPeptidesspa
dc.subject.keywordPlasmodium falciparumspa
dc.subject.keywordProtein bindingspa
dc.subject.keywordProtein conformationspa
dc.subject.keywordProtein interaction domains and motifsspa
dc.subject.keywordProtozoan proteinsspa
dc.subject.keywordRabbitsspa
dc.subject.keywordRatsspa
dc.subject.keywordPlasmodium falciparumspa
dc.titleA Sequence in Subdomain 2 of DBL1? of Plasmodium falciparum Erythrocyte Membrane Protein 1 Induces Strain Transcending Antibodiesspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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