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Fitting modified HRP-I peptide analogue 3D structure into HLA-DR molecules induces protection against Plasmodium falciparum malaria
dc.creator | Bermudez, Adriana | |
dc.creator | Alba, Patricia | spa |
dc.creator | Espejo, Fabiola | spa |
dc.creator | Vargas, Luis Eduardo | spa |
dc.creator | Parra, Carlos | spa |
dc.creator | Rodríguez, Raúl | spa |
dc.creator | Reyes, Claudia | spa |
dc.creator | Patarroyo, Manuel Elkin | spa |
dc.date.accessioned | 2020-08-06T16:20:16Z | |
dc.date.available | 2020-08-06T16:20:16Z | |
dc.date.created | 2005-02 | spa |
dc.description.abstract | Conserved, high-activity, red blood cell binding malaria peptide 6786, from the HRP-I protein, having a random 3D structure as determined by 1H-NMR, was non-immunogenic and non-protection inducing when used as an immunogen in Aotus monkeys. Modifications made in its amino acid sequence were thus performed to render it immunogenic and protection inducing. Non-immunogenic, non-protection inducing modified peptide 13852 presented A2-H8 and K14-L18 helix fragments. Immunogenic, non-protection inducing modified peptide 23428 presented a short, displaced helix in a different region, whilst immunogenic, protection inducing peptide 24224 had 2 displaced helical regions towards the central region giving more flexibility to its N- and C-terminals. Immunogenic and protection inducing peptides bound with high affinity to HLA-DRB1 0301 whilst others did not bind to any HLA-DRB1 purified molecule. Structural modifications may thus lead to inducing immunogenicity and protection associated with their capacity to bind specifically to purified HLA-DRB1 molecules, suggesting a new way of developing multi-component, subunit-based malarial vaccines. | eng |
dc.format.mimetype | application/pdf | |
dc.identifier.doi | https://doi.org/10.1016/j.biocel.2004.07.012 | |
dc.identifier.issn | ISSN: 1357-2725 | |
dc.identifier.issn | EISSN: 0020-711X | |
dc.identifier.uri | https://repository.urosario.edu.co/handle/10336/25938 | |
dc.language.iso | eng | spa |
dc.publisher | Elsevier | spa |
dc.relation.citationEndPage | 349 | |
dc.relation.citationIssue | No. 2 | |
dc.relation.citationStartPage | 336 | |
dc.relation.citationTitle | International Journal of Biochemistry and Cell Biology, International Journal of Biochemistry | |
dc.relation.citationVolume | Vol. 37 | |
dc.relation.ispartof | International Journal of Biochemistry and Cell Biology, ISSN:1357-2725;EISSN:0020-711X, Vol.37 No.2 (2005);pp.336-349 | spa |
dc.relation.uri | https://www.sciencedirect.com/science/article/abs/pii/S1357272504002602 | spa |
dc.rights.accesRights | info:eu-repo/semantics/restrictedAccess | |
dc.rights.acceso | Restringido (Acceso a grupos específicos) | spa |
dc.source | International Journal of Biochemistry and Cell Biology, International Journal of Biochemistry | spa |
dc.source.instname | instname:Universidad del Rosario | |
dc.source.reponame | reponame:Repositorio Institucional EdocUR | |
dc.subject.keyword | Malaria | spa |
dc.subject.keyword | KAHRP-I | spa |
dc.subject.keyword | NMR | spa |
dc.subject.keyword | Structural calculations | spa |
dc.subject.keyword | HLA-DR | spa |
dc.title | Fitting modified HRP-I peptide analogue 3D structure into HLA-DR molecules induces protection against Plasmodium falciparum malaria | spa |
dc.title.TranslatedTitle | La adaptación de la estructura 3D análoga del péptido HRP-I modificado en las moléculas HLA-DR induce protección contra la malaria por Plasmodium falciparum | spa |
dc.type | article | eng |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | |
dc.type.spa | Artículo | spa |