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Fitting modified HRP-I peptide analogue 3D structure into HLA-DR molecules induces protection against Plasmodium falciparum malaria

dc.creatorBermudez, Adriana
dc.creatorAlba, Patriciaspa
dc.creatorEspejo, Fabiolaspa
dc.creatorVargas, Luis Eduardospa
dc.creatorParra, Carlosspa
dc.creatorRodríguez, Raúlspa
dc.creatorReyes, Claudiaspa
dc.creatorPatarroyo, Manuel Elkinspa
dc.date.accessioned2020-08-06T16:20:16Z
dc.date.available2020-08-06T16:20:16Z
dc.date.created2005-02spa
dc.description.abstractConserved, high-activity, red blood cell binding malaria peptide 6786, from the HRP-I protein, having a random 3D structure as determined by 1H-NMR, was non-immunogenic and non-protection inducing when used as an immunogen in Aotus monkeys. Modifications made in its amino acid sequence were thus performed to render it immunogenic and protection inducing. Non-immunogenic, non-protection inducing modified peptide 13852 presented A2-H8 and K14-L18 helix fragments. Immunogenic, non-protection inducing modified peptide 23428 presented a short, displaced helix in a different region, whilst immunogenic, protection inducing peptide 24224 had 2 displaced helical regions towards the central region giving more flexibility to its N- and C-terminals. Immunogenic and protection inducing peptides bound with high affinity to HLA-DRB1 0301 whilst others did not bind to any HLA-DRB1 purified molecule. Structural modifications may thus lead to inducing immunogenicity and protection associated with their capacity to bind specifically to purified HLA-DRB1 molecules, suggesting a new way of developing multi-component, subunit-based malarial vaccines.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.biocel.2004.07.012
dc.identifier.issnISSN: 1357-2725
dc.identifier.issnEISSN: 0020-711X
dc.identifier.urihttps://repository.urosario.edu.co/handle/10336/25938
dc.language.isoengspa
dc.publisherElsevierspa
dc.relation.citationEndPage349
dc.relation.citationIssueNo. 2
dc.relation.citationStartPage336
dc.relation.citationTitleInternational Journal of Biochemistry and Cell Biology, International Journal of Biochemistry
dc.relation.citationVolumeVol. 37
dc.relation.ispartofInternational Journal of Biochemistry and Cell Biology, ISSN:1357-2725;EISSN:0020-711X, Vol.37 No.2 (2005);pp.336-349spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S1357272504002602spa
dc.rights.accesRightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.accesoRestringido (Acceso a grupos específicos)spa
dc.sourceInternational Journal of Biochemistry and Cell Biology, International Journal of Biochemistryspa
dc.source.instnameinstname:Universidad del Rosario
dc.source.reponamereponame:Repositorio Institucional EdocUR
dc.subject.keywordMalariaspa
dc.subject.keywordKAHRP-Ispa
dc.subject.keywordNMRspa
dc.subject.keywordStructural calculationsspa
dc.subject.keywordHLA-DRspa
dc.titleFitting modified HRP-I peptide analogue 3D structure into HLA-DR molecules induces protection against Plasmodium falciparum malariaspa
dc.title.TranslatedTitleLa adaptación de la estructura 3D análoga del péptido HRP-I modificado en las moléculas HLA-DR induce protección contra la malaria por Plasmodium falciparumspa
dc.typearticleeng
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersion
dc.type.spaArtículospa
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